RESUMO
Objective: To detect the expression of sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 (SPOCK 1) gene in bladder cancer cells, and further to investigate the effects of down-regulation of SPOCK 1 gene expression on the proliferation, migration and invasion of bladder cancer cells. Methods: The expression level of SPOCK 1 gene in human ureteral epithelial immortalized cell line SV-HUC-1 and bladder cancer cell lines 5637 and T24 was detected by real-time fluorescent quantitative PCR (RFQ-PCR) and Western blotting, respectively. The siRNA fragments targeting SPCOK 1 gene were constructed and transfected into bladder cancer 5637 cells by liposome (as the experimental group); at the same time, the 5637 cells were transfected without anything as the blank control group and transfected with scramble siRNA as the negative control group. Then the expressions of SPOCK1 mRNA and protein in each group were detected by RFQ-PCR and Western blotting, respectively. The changes of proliferation, migration and invasion abilities of bladder cancer 5637 cells after SPOCK1 expression down-regulation were detected by CCK-8 method, Wound healing assay and Transwell chamber assay, respectively. Results: As compared with the ureteral epithelial immortalized cells, the expression levels of SPOCK1 mRNA and protein were higher in bladder cancer cells, especially in 5637 cells (both P < 0.05). After transfection with SPOCK1 siRNA, the expression of SPOCK 1 gene was downregulated (P < 0.05), the proliferation, migration and invasion of bladder cancer 5637 cells were significantly inhibited (all P < 0.05). Conclusion: SPOCK 1 gene is overexpressed in bladder cancer cells. The down-regulation of SPOCK 1 gene expression can reduce the proliferation, migration and invasion of bladder cancer 5637 cells.
RESUMO
Objective To investigate and evaluate the clinical signiifcance of lfexible ureteroscopy in computed tomography urography negative patients with upper urinary tract hematuria. To improve the diagnostic rate of patients with upper urinary tract hematuria. Methods We retrospectively reviewed the cases of 10 computed tomography urography negative patients with upper urinary tract hematuria. The age ranges from 38 to 75 years old, and the average of them is 45.2 years old. The patients consist of 7 male patients and 3 female patients. Among them, 4 cases were on the left upper urinary tract cases, 6 cases were right upper urinary tract. All patients received lfexible ureteroscopy to conifrm the diagnosis and take the relevant treatment. Results In the total of 10 patients, 3 patients (30%) were diagnosed with tumors of renal pelvis, a renal arteriovenous ifstula was found in 1 case (10%), 3 patients (30%) were considered non-specific inflammation, other 3 patients (30%) had no obvious abnormalities. Conclusions Flexible ureteroscopy has significantly clinical diagnosis in computed tomography urography negative patients with upper urinary tract hematuria.