RESUMO
[Objective] The biocompatibility between SGBG/PHBV and human PDLC were investigated to provide a basis for the choice of the scaff material of periodontal tissue engineering.[Methods] Human PDLC were cultured using tissue-explant technique.Seeding on 96 wells plate,9 wells for one group,Four different concentrations (100％,75％,50％,25％,0％) of maceration extract of SGBG/PHBV were added into the culture plantsafter 48-h cell seeding,the grades of the cytotoxicity of SGBG/PHBV was evaluated by MTT assay.Human PDLC cultured in vitro were collected and seeded on the three-dimensional scaffolds of SGBG/PH-BV,the cellular morphology and cell growth on the scaffolds were observed and photographed by scanning electronic microscope.HumanPDLC seeded on the three-dimensional scaffolds of SGBG/PHBV in the experimental group,and human PDLC seeded by DMEM in the control group,after 12-,24-,and 48-h cell seeding,got 27 simples for each group,and the affection of the SGBG/PHBV on cell secretory function was observed by spectrophotometry which assayed the biochemical indexes ALP in supinate.[Results] The grades of the cytotoxicity of SGBG/PHBV were 0 and 1.It was displayed that human PDLC adhered and proliferated well on the scaffold of SGBG/PHBV under the scanning electronic microscope.The significant difference of ALP in supinate between the experimental group and the control group (P ＜ 0.05).[Conclusions] SGBG/PHBV had no cytotoxicity to human PDLC.SGBG/PHBV is potential to further study as the scaffolds of periodontal tissue engineering since it displayed the satisfactory biocompatibility with human PDLC.
RESUMO
BACKGROUND:NovaMin is a kind of bioactive glass, and its active ingredients react with saliva to form hydroxyapatite crystals, which deposit on the surface of the teeth and can close the exposed dentinal tubules. It can be used for the treatment of dentin hypersensitivity. OBJECTIVE:To study the effect of toothpaste containing NovaMin on tooth hypersensitivity patients after subgingival scaling and root planing surgery. METHODS:According to inclusion and exclusion criteria, 96 patients were picked out from 543 patients to participate in the experiment. In compliance with the principle of randomized, double-blind, comparison, 98 subjects were randomly divided into two groups:experiment group in which the toothpaste containing NovaMin was used, and control group in which the toothpaste without NovaMin was used. Visual analog scale scoring was used to evaluate the degree of sensitivity to air stimulus respectively before and 4 weeks after al ergy experiment. RESULTS AND CONCLUSION:Total y 88 patients completed the experiment. After treatment with toothpaste for 4 weeks, visual analog scale scores in the experiment group decreased from 4.25±1.87 at baseline to 2.53±1.22 by air stimulus, and visual analog scale scores in the control group decreased from 4.03±1.92 at baseline to 3.63±1.45. The visual analog scale scores in the experiment group and control group both reduced, and the difference was with statistics significance (P<0.05). Compared with the control group, visual analog scale scores in the experiment group decreased more significantly. It showed that the toothpaste containing NovaMin has significant effect on dentin hypersensitivity after subgingival scaling and root planing surgery.