Mechanism of ablation with nanosecond pulsed electric field / 浙江大学学报·医学版

Nanosecond pulsed electric field ablation has been widely applied in clinical cancer treatment, while its molecular mechanism is still unclear. Researchers have revealed that nanosecond pulsed electric field generates nanopores in plasma membrane, leading to a rapid influx of Ca²⁺; it has specific effect on intracellular organelle membranes, resulting in endoplasmic reticulum injuries and mitochondrial membrane potential changes. In addition, it may also change cellular morphology through damage of cytoskeleton. This article reviews the recent research advances on the molecular mechanism of cell membrane and organelle changes induced by nanosecond pulsed electric field ablation.

Research advances of microRNA in corneaI neovascuIarization / 国际眼科杂志(Guoji Yanke Zazhi)

·Corneal neovascularization ( CNV) is one of the most important causes that affecting corneal transparency, and it is a high risk factor of allogeneic corneal graft rejection. lt has become a research focus for the regulation of CNV. microRNAs are a class of endogenous non-protein-coding micromolecule RNAs which play a critical role in regulating a series of life process.Researches in recent years show a close correlation between microRNA and CNV.ln this article we reviewed the recent advances in these researches.

The genotyping of clarithromycin-resistant Hp isolates from patients in the west of Guangxi / 中华检验医学杂志

Objective To study the genotyping of clarithromycin-resistant Helicobacter pylori(Hp) isolates.Methods From March 2007 to October 2007,247 gastric mucosa specimens were collected by the endoscopy from the patients with peptic ulcer or gastritis at the affiliated hospital of Youjiang Medical College for Nationalities.A total of 126 Hp strains were isolated.Resistance to clarithromycin in Hp was determined by E-test.All of the resistant isolates were genotyped with REP-PCR and further clustered with NTsys_2 software.The clinical data was collected for these patients with clarithromycin-resistant Hp.Results The 26 clarithromycin-resistant isolates from the west of Guangxi were divided into six genotypea including Group Ⅰ,Group Ⅱ,Group Ⅲ,Group Ⅲ,Group Ⅳ,Group Ⅴ and Group Ⅵ according to the homology of 78%.Every group have 2,11,1,8,3,1 strains Hp,respectively.Strains of group Ⅱ were isolated from patients with peptic ulcer and most of them from Chuang patients.All strains of group Ⅳ were isolated from patients with gastritis.Conclusions The clarithromycin-resistant isolates were divided into six groups by REP-PCR Disease type.nationality of patients and family history of stomach diseases were associated with the genotypes.

Analysis of the resistance of Helicobacter pylori strains to clarithromycin in west-Guangxi region / 中华微生物学和免疫学杂志

objective To analyze the prevalence of Helicobacter pylori(Hp)strains resistant to clarithromycin and the relation of 23S rRNA genetic mutation with clarithromycin resistance.Methotis Hp strains were isolated from gastric mucosa biopsies of patients with peptic ulcer or gastritis.Resistanee of the isolates to elarithromycin wag determined using diffusion test.Mutations in elarithromyein resistant strains were identified by PCR-RFLP and gene sequencing.Results The prevalence of Hp strains resistant clarithromyein was 22.2%.10 of clarithromycin resistant strains had A2143G.A2144G point mutation in 23S rRNA gene,and none of the 10 susceptible strains had the A2143G or A2144G mutation,and the result was affirmed by measure sequencing.Conclusion The prevalence of Hp is higher than Beijing and Shanghai,mutations in 23S rRNA are associated with elarithromyein resistance in Hp.