RESUMO
Background : Demonstration of sex chromatin forms an important aspect of human genetics. It also establishes the interrelationship between sex chromatin and an inactive X-chromosome. The term “sex chromatin” in blood refers to the “Drumsticks of polymorphonuclear leukocytes” or “Davidson’s bodies”. Objective: This correlative study evaluates the presence of these drumsticks quantitatively and also highlights the concept of blood chimaerism in humans. Method: Leishman-stained peripheral blood smears from 60 individuals (30 males and 30 females) were obtained and studied under bright-field microscope (40X) for presence of Drumstick appendages. Results and conclusion: On comparing mean numbers of Davidson’s bodies in females and males, an extremely significant correlation (P<0.0001) was seen. Hence, it could be surmised that the presence of appendages in neutrophils (Drumstick bodies) can be useful in gender differentiation.
RESUMO
Aim: Mast cells have been hypothesized to play a significant role in pathogenesis of odontogenic cysts. The aim of this study was to evaluate mast cell distribution in cystic lining and the capsule to formulate a mechanism of cystic expansion. Methods: Ten formalin-fixed paraffin embedded tissue blocks each of OKC, dentigerous and radicular cysts were selected. Toluidine blue staining (1% in 1% NaCl solution) was done in 5µm thick sections and counting performed in 10 areas using an ocular grid. Areas counted were divided into 4 zones: intraepithelial, subepithelial, intermediate and deep zones (Group I, II, III and IV respectively). Statistical analysis: Mean ±S.D. was calculated in each group followed by paired T test. Results: Mast cells had greatest concentration in subepithelial zone. T test showed no significant differences between group I and II zones in OKC but a highly significant difference between groups I and II in dentigerous cyst. Radicular cysts showed a significant difference between groups II and III. Conclusion: Mast cell degranulation releases numerous hydrolytic enzymes that facilitate breakdown of capsular matrix increasing the hydrostatic pressure due to raised osmolality. Influx of tissue fluids results in their enlargement coupled with resorption at the bone-cyst interface.