In situ synthesis of a spherical covalent organic framework as a stationary phase for capillary electrochromatography / 药物分析学报

Covalent organic frameworks(COFs)are a novel type of crystalline porous organic polymer materials recently developed.It has several advantages in chromatographic separation field,such as high thermal stability,porosity,structural regularity,and large specific surface area.Here,a novel spherical COF 1,3,5-tris(4-aminophenyl)benzene(TAPB)and 2,5-bis(2-propyn-1-yloxy)-1,4-benzenedicarboxaldehyde(BPTA)was developed as an electrochromatographic stationary phase for capillary electro-chromatography separation.The COFTTAPB-BPTA modified capillary column was fabricated via a facile in situ growth method at room temperature.The characterization results of scanning electron microscopy(SEM),Fourier transform infrared(FT-IR)spectroscopy,and X-ray diffraction(XRD)confirmed that COF TAPB-BPTA were successfully modified onto the capillary inner surface.The electrochromatography separation performance of the COF TAPB-BPTA modified capillary was investigated.The prepared column demonstrated outstanding separation performance toward alkylbenzenes,phenols,and chlorobenzenes compounds.Furthermore,the baseline separations of non-steroidal anti-inflammatory drugs(NSAIDs)and parabens with good efficiency and high resolution were achieved.Also,the prepared column possessed satisfactory precision of the intra-day runs(n=5),inter-day runs(n=3),and parallel columns(n=3),and the relative standard deviations(RSDs)of the retention times of tested alkylbenzenes were all less than 2.58％.Thus,this new COF-based stationary phase shows tremendous application potential in chromatographic separation field.

Physicochemical degradation of phycocyanin and means to improve its stability:A short review / 药物分析学报

The cyanobacterium Arthrospira platensis,spirulina,is a source of pigments such as phycobiliprotein and phycocyanin.Phycocyanin is used in the food,cosmetics,and pharmaceutical industries because of its antioxidant,anti-inflammatory,and anticancer properties.The different steps involved in extraction and purification of this protein can alter the final properties.In this review,the stability of phycocyanin(pH,temperature,and light)is discussed,considering the physicochemical parameters of kinetic modeling.The optimal working pH range for phycocyanin is between 5.5 and 6.0 and it remains stable up to 45℃;however,exposure to relatively high temperatures or acidic pH decreases its half-life and increases the degradation kinetic constant.Phycobiliproteins are sensitive to light;preservatives such as mono-and di-saccharides,citric acid,or sodium chloride appear to be effective stabilizing agents.Encapsulation within nano-or micro-structured materials such as nanofibers,microparticles,or nanoparticles,can also pre-serve or enhance its stability.

Determination of bioactive components in the fruits of Cercis chinensis Bunge by HPLC-MS/MS and quality evaluation by principal components and hierarchical cluster analyses / 药物分析学报

The fruits of leguminous plants Cercis Chinensis Bunge are still overlooked although they have been reported to be antioxidative because of the limited information on the phytochemicals of C.chinensis fruits.A simple,rapid and sensitive HPLC-MS/MS method was developed for the identification and quantitation of the major bioactive components in C.chinensis fruits.Eighteen polyphenols were iden-tified,which are first reported in C.chinensis fruits.Moreover,ten components were simultaneously quantified.The validated quantitative method was proved to be sensitive,reproducible and accurate.Then,it was applied to analyze batches of C.chinensis fruits from different phytomorph and areas.The principal components analysis (PCA) realized visualization and reduction of data set dimension while the hierarchical cluster analysis (HCA) indicated that the content of phenolic acids or all ten components might be used to differentiate C.chinensis fruits of different phytomorph.

Identification and quantification of the bioactive components in Osmanthus fragrans roots by HPLC-MS/MS / 药物分析学报

The roots of O.fragrans are also a valuable resource in addition to its flowers and fruits.In this study,the HPLC-MS/MS method used for analyzing the chemical constituents in O.fragrans roots extract was developed,which showed high sensitivity for both qualitative and quantitative analyses.Thirty-two compounds were first discovered in O.fragrans roots,one compound of which was reported for the first time.The simultaneous determination method for acteoside,isoacteoside,oleuropein and phillyrin was validated to be sensitive and accurate.Then it was applied to determine the content of bioactive components in O.fragrans roots from different cultivars.The content of oleuropein and phillyrin in the twelve batches was relatively stable,while the content of acteoside and isoacteoside varied greatly.Moreover,the therapeutic material basis and mechanism of O.fragrans roots exerting its traditional pharmacodynamics were analyzed by network pharmacology.The results showed that O.fragrans roots might be effective for the treatment of inflammation,cardiovascular diseases,cancer,and rheumatoid arthritis,which is consistent with the traditional pharmacodynamics of O.fragrans roots.This work can provide an analytical method for the comprehensive development of O.fragrans roots.