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Journal of Zhejiang University. Science. B ; (12): 960-972, 2018.
Artigo em Inglês | WPRIM | ID: wpr-1010436

RESUMO

Royal jelly (RJ) from honeybee has been widely used as a health promotion supplement. The major royal jelly proteins (MRJPs) have been identified as the functional component of RJ. However, the question of whether MRJPs have anti-senescence activity for human cells remains. Human embryonic lung fibroblast (HFL-I) cells were cultured in media containing no MRJPs (A), MRJPs at 0.1 mg/ml (B), 0.2 mg/ml (C), or 0.3 mg/ml (D), or bovine serum albumin (BSA) at 0.2 mg/ml (E). The mean population doubling levels of cells in media B, C, D, and E were increased by 12.4%, 31.2%, 24.0%, and 10.4%, respectively, compared with that in medium A. The cells in medium C also exhibited the highest relative proliferation activity, the lowest senescence, and the longest telomeres. Moreover, MRJPs up-regulated the expression of superoxide dismutase-1 (SOD1) and down-regulated the expression of mammalian target of rapamycin (MTOR), catenin beta like-1 (CTNNB1), and tumor protein p53 (TP53). Raman spectra analysis showed that there were two unique bands related to DNA synthesis materials, amide carbonyl group vibrations and aromatic hydrogens. These results suggest that MRJPs possess anti-senescence activity for the HFL-I cell line, and provide new knowledge illustrating the molecular mechanism of MRJPs as anti-senescence factors.


Assuntos
Animais , Bovinos , Humanos , Abelhas , Linhagem Celular , Proliferação de Células , Senescência Celular/efeitos dos fármacos , Meios de Cultura , Relação Dose-Resposta a Droga , Ácidos Graxos/química , Fibroblastos/efeitos dos fármacos , Proteínas de Insetos/química , Pulmão/efeitos dos fármacos , Albumina Sérica/metabolismo , Análise Espectral Raman , Superóxido Dismutase-1/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Proteína Supressora de Tumor p53/metabolismo , beta Catenina/metabolismo
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