RESUMO
Objective: To optimize the processing technology of Gardenia jasminoides carbon and validate the process with pharmacodynamics research. Methods: The pyrolysis characteristics of different active components in G. jasminoides were analyzed by thermal analysis technology, and the optimum processing temperature range of G. jasminoides carbon was obtained; Taking the tannin content in G. jasminoides carbon as the index, two factors of stir-frying temperature and stir-frying time was chosen to optimize the processing technology of G. jasminoides carbon by response surface methodology, and the processing technology of G. jasminoides carbon was verified by pharmacodynamics experiment (hemostasis test). Results: The optimum processing temperature range of G. jasminoides carbon was 290.3-387 ℃, response surface methodology was used to optimize the processing technology of G. jasminoides carbon: The processing temperature was 330 ℃, the processing time was 5.91 min, and the tannin content was 3.120 mg/g; Compared with the blank group, the new method group and the traditional group could significantly shorten the clotting time of mice (P < 0.01), raw product group can significantly shorten the clotting time of mice (P < 0.05), the new method group can significantly shorten APTT in mice (P < 0.05), there was no significant difference in PT and TT in mice. Conclusion: The processing technology of G. jasminoides carbon was optimized by thermal analysis technology and response surface methodology, and the results were verified by pharmacodynamics experiments. The method can provide a reference for improving the processing technology of Chinese materia medica and quantifying the fire parameters in the process of processing.