Studies on chemical constituents in bark of dictamnus dasycarpus / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents in bark of Dictamnus dasycarpus.</p><p><b>METHOD</b>Isolation and purification were carried out on silica gel column chromatography, prepared thin layer chromatography and sephadex LH - 20, et al. The structures were identified by spectral analysis.</p><p><b>RESULT</b>Twelve compounds were obtained from bark of D. dasycarpus and the structures were determined as dictamnine (I), fraxinellone (II), skimmianine (III), gamma-fagarine (IV ), beta-sitosterol (V), obacunone (VI), limonin disophenol (VII), fraxinellonone (VIII), wogonin (IX), rutevin (X), kihadinin B (XI), dasycarine (XII).</p><p><b>CONCLUSION</b>Compounds IX and XI were isolated from genus Dictamnus for the first time, and compound VIII was isolated from the species for the first time.</p>

Determination of lignan glycosides in Gaultheria leucocarpa var. yunnanensis by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To control the quality of Gaultheria leucocarpa var. yunnanensis the contents of lignans in these plants from different provinces were detected.</p><p><b>METHOD</b>HPLC was adopted using Hypersil-18 column (0.4 cm x 25 cm), methanol: acetonitrile: water (30:5:65) as mobile phase.</p><p><b>RESULT</b>The calibration curves of D1(gaultherinA), D2[(-)-5'-methoxyisolariciresinol-2 alpha-O-beta-D-xylopyranoside)], D4[(+)-lyoniresinol-2 alpha-O-beta-D-glucopyranoside)] were linear in the range of 0.10-0.40 microgram, 0.03-0.13 microgram, 0.02-0.09 microgram, respectively. The average recovery of D1 was 98.9% and RSD was 2.6%.</p><p><b>CONCLUSION</b>Comparing the samples from Jinxiou(158.2 mg.100 g)-1 and Kumming(154.8 mg.100 g)-1, that of Guiyang showed the highest content of LD(208.8 mg.100 g)-1. The content of LD in the tender above-ground part was 1.3 times higher than that in the wilt.</p>