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"Rain classroom + " is an online and offline teaching mode developed under the background of "Internet + Education". However, there are still certain shortcomings in online teaching. Based on this situation, we explore the application of a new mixed teaching mode based on "Rain classroom + " in the teaching of Pathophysiology. Centered on three links of pre-class, in-class and post-class, the flipped classroom has been realized through teaching interaction by combining Tencent Meeting and integrating the problem-based learning with the case-based learning (PBL/CBL) in the teaching. "Rain classroom + " mixed teaching mode realizes the "student-centered" teaching concept by optimizing teaching resources, reorganizing teaching content and perfecting teaching design.
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Objective To detect the effects and mechanisms of CD147 in thyroid cancer cell SW579. Methods RT-PCR and Western-blot were used to determine the levels of CD147 mRNA and protein in SW579 cells after treated with CD147 specific small interference RNA (siRNA). The proliferative ratio of SW579 cells after treated with CD147 siRNA was detected by CCK-8 assay. We detected the changes of cellular mobility after treated with CD147 siRNA by transwell assay. Mobility related proteins were analyzed by western-blot. Results The proliferative ratio and the mobility of SW579 cells were inhibited significantly by down-regulation of CD147. The levels of MMP-2 and MMP-9 were lower in treated cells than untreated ones. Expression of Wave 2 and a mobility related protein were decreased in accompany with down-regulation of CD147. Conclusion Down-regulation of CD147 inhibits mobility of SW579 by suppressing MMP-2, MMP-9 and WAVE 2.
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There existed animal ethical issues in the process of functional experiment including poor breeding environment before animal experiment, insufficient prepare before class, not standard experimental operation, less attention paid to the study of anesthesia, disposal on animals after the experiment. Based on the analysis of these issues and combined with the thought of respect for animals′rights and attention to animal welfare, the authors put forward suggestions from the aspects including the schools′active attention and support, curriculum, standardizing the experimental design and operation steps, improving the animal experiment teachers′ethical consciousness, cul-tivating the students′experimental animal ethical consciousness. In addition, this paper put forward the thinking of further strengthening education:the domestic medical colleges generally need to strengthen medical ethics educa-tion, and should adopt various forms to cultivate medical students′medical ethics quality of treasuring life.
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Objective To measure the expression of SFRP1 in bladder cancer and explore the corresponding mechanism,in order to study the roles of SFRP1 in the pathogenesis of bladder cancer.Methods SFRP1 mRNA was detected by RT-PCR.Methylation status of SFRP1 was detected by methylation specific PCR.SFRP1 protein was determined by Western blotting.Results SFRP1 was methylated in bladder cancer cell lines T24 and 5637,not in SCaBER.SFRP1 mRNA and protein were detected in SCaBER,but not in T24 and 5637.Six hours after treating T24 and 5637 with 5'-aza-deoxycytidine,both T24 and 5637 expressed SFRP1 mRNA and protein.In 45 bladder cancers,methylation of SFRP1 was detected in 28 (62.2％).In matched cancer adjacent tissues,6 (13.3％) were found with SFRP1 methylation.The methylation rate in bladder cancers was significant higher than that in matched cancer adjacent tissues (P ＜ 0.01).Conclusions SFRP1 is downregulated in some bladder cancers due to methylation.SFRP1 methylation and aberrant expression may be involved in the pathogenesis of bladder cancers.
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AIM:To determine the methylation and expression of Wnt antagonist secreted Frizzled-related protein-2(SFRP2) in gastric cancer and to explore the role of SFRP2 in gastric carcinogenesis.METHODS:Methylation status of SFRP2 was detected by methylation-specific PCR(MSP).Real-time PCR was used to determine the expression of SFRP2 in gastric cancers and matched cancer adjacent normal tissues.SFRP2 expression in gastric cancer cell lines was determined by reverse-transcriptional PCR(RT-PCR) and Western blotting.RESULTS:SFRP2 methylation was found in 26(65%) gastric cancers and in 3(7.5%) matched cancer adjacent normal tissues.The frequency of methylation for SFRP2 was significantly higher in gastric cancers than that in matched normal tissues(x2=28.614,P