Expression of HIF-2α and VEGF in colorectal cancer and its relationship with prognosis / 重庆医学

Objective To study the expression of HIF-2α and VEGF in colorectal cancer and to investigate the relationship between them and clinicopathologic parameter.Methods Immunohistochemistry staining was conducted to detect the expression of HIF-2α and VEGF protein in 67 samples of colorectal tumor tissues and 67 samples of normal adjacent tissue.Results The expression of HIF-2α and VEGF in colorectal cancer tissues was significantly higher than that in adjacent tissues.The expression of HIF-2α and VEGF increased with the clinical stage and lymph node metastasis.The expression of HIF-2α increased with the tumor volume.The expression of HIF-2α and VEGF was not related to the age,sex,tumor location and tumor differentiation of the patients.HIF-2α was positively correlated with VEGF expression.Kaplan-Meier survival analysis showed that HIF-2α expression was associated with survival,that is,the higher expression of HIF-2α the worse of prognosis was obtained.Conclusion HIF-2α is involved in the process of growth,invasion and metastasis of colorectal cancer.This process may be related to the regulation of VEGF expression.

Relationship between inflammatory factors and carotid atherosclerosis in OSAHS patients with type 2 diabetes mellitus / 重庆医学

Objective To investigate the relationship between serum inflammatory cytokines and atherosclerosis in type 2 diabetic patients with obstructive sleep apnea hypopnea syndrome (OSAHS).Methods One hundred and three patients with type 2 diabetes were divided into OSAHS group (OSAHS group) and type 2 diabetes mellitus group (non-OSAHS group) according to the results of polysomnography(PSG),and 35 healthy subjects were selected as control group.Serum levels of total cholesterol,triglyceride,fasting plasma glucose(FPG),glycosylated hemoglobin(HbA1c),tumor necrosis factor-α(TNF-α),high-sensitivity C-reactive protein (hs-CRP) and carotid intima-media thickness (IMT) were measured,and their relationship with OSAHS were analyzed.Results The waist circumference,BMI,FPG,HbA1c,IMT,TNF-α and CRP in OSAHS group were significantly higher than those in non-OSAHS group (P＜0.05).Logistic regression analysis showed that,IMT was independently associated with TNF-α and hs-CRP.Conclusion Patients of T2DM with OSAHS have poor blood glucose control and higher incidence of atherosclerosis.High levels of TNF-α and hs-CRP may be involved in the formation of atherosclerosis and plaque occurrence and development.

Detection of peripheral NGAL and MMP9 levels and its clinical significance in primary hepatic carcinoma / 重庆医学

Objective To explore the changes of matrix metalloproteinase 9(MMP9) and neutrophil gelatinase associated li-pocalin(NGAL) in primary hepatic carcinoma and its clinical significance .Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum level of MMP9 and NGAL in 47 patients with primary hepatic carcinoma and 19 healthy controls .The association between serum MMP9 and NGAL levels with the clinicopathological features of hepatic carcinoma were evaluated .ROC curves were used to analyze the influence of MMP9 and NGAL on the diagnostic value .Kaplan-Meier method was used to evaluate the relationship between the expressions of NGAL and MM P9 and the prognosis of patients with HCC .Results Serum MMP9 and NGAL levels in hepatic carcinoma patients are significantly higher compared with normal controls (P ≤ 0 .05) .The serum level of MMP9 and NGAL in primary hepatic carcinoma was positively correlated with TNM stage (P≤ 0 .05) .The sensitivity and specifici-ty of MMP9 and NGAL for primary hepatic carcinoma were 70 .2% and 83 .0% .The sensitivity of the joint detection were 94 .9% . Kaplan Meier survival curves showed that cumulative survival rates of patients with positive expression of NGAL were significantly lower than those of patients with negative expression .Conclusion MM P9 and NGAL might play an important role in gastric cancer generation and development .Monitoring of serum MMP9 and NGAL levels might be helpful for diagnosis and evaluation of progno-sis for primary hepatic carcinoma .