RESUMO
OBJCTIVE:To determine the content of Gentiopicrin in Qianggan capsules by HPLC.METHODS:The sample was separated on Diamonsil TM C18 (200 mm?4.6 mm,5 ?m) with mobile phase consisted of methanol-water (23∶77) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 254 nm.RESULTS: The linear range of Gentiopicrin was 0.018 59～0.232 40 ?g(r=0.999 6) with an average recovery of 96.59%(RSD=1.35%,n=6).CONCLUSION: The meth-od developed is accurate,stable and reproducible,and it provides basis for the improvement of the quality standard of Qianggan capsules.
RESUMO
AIM To develop a method to determine rhien,emodin and chrysophanol contents in Dahuang Zhechong Pills (Radix et Rhizoma Rhei Preparata,Eupolyphaga seu Steleophaga,Hirudo,etc.). METHODS HPLC was used to determine rhien,emodin and chrysophanol contents in Dahuang Zhechong Pills.The separation was performed on SYMMETRY ○ column with methol-phosphate acid(0.1 % ) (6∶ 1) as a mobile phase and wavelength of UV detector was at 254 nm. RESULTS The resolution and the linearity of the method were good with the avevage recovery of rhien:101.92, RSD was 2.01 % ;emodin:97.74 % , RSD was 0.78 % ;chrysophanol, 98.66 % , RSD was 2.41 % ;respectively. CONCLUSION The method is simple and rapid and with satisfactoty results.It can be suitable for quality control of Dahuang Zhechong Pills.