RESUMO
ABSTRACT OBJECTIVE：To study pharmacokinetic characteristics of single dose and multiple dose administration of Gefitinib emulsion in rats. METHODS：The rats were divided into single administration group and multiple administration group. Single administration group was subdivided into Gefitinib raw medicine group（50 mg/kg，i.g.）and Gefitinib emulsion group（50 mg/kg，i.g.），with 6 rats in each group，gavage once. Multiple administration group were subdivided into Gefitinib raw medicine group （50 mg/kg）and Gefitinib emulsion group（50 mg/kg），with 8 rats in each group；they were given relevant medicine intragastricaly for consecutive 7d，once a day. 0.3 mL blood of rats in Gefitinib raw medicine group was taken before medication and 1，2，2.5， 3，3.5，3.75，4，4.25，4.5，6，8，12 and 24 h after medication；0.3 mL blood of rats in Gefitinib emulsion group was taken before medication and 2，4，6，8，9，10，11，12，13，14，16，24，36 and 48 h after administration（Multiple administration group is after 7 d of administration）. HPLC method was used to determine the plasma concentration of gefitinib in rat，and plasma concentration-time curves were drawn. Pharmacokinetic parameters were fitted by using DAS 2.0 software. RESULTS：After single administration，compared with the tmax（[ 2.67±0.75）h]，MRT0-24 h （[ 8.68±0.91）h]，MRT0- ∞ （[ 14.20±3.45）h] of Gefitinib raw medicine group，tmax （[ 8.33±4.41）h]，MRT0-48 h （[ 15.00±1.60）h]，MRT0-∞ （[ 17.60±2.66）h] of Gefitinib emulsion group were increased significantly（P＜0.05）. After multiple administration，compared with the tmax （[ 6.79±3.75）h]，AUC0-48 h （[ 41.10±8.92） mg·h/L]，Vz/F [（16.30±5.45）L/kg]，CLz/F [（0.94±0.19） L/（h·kg）]，MRT0-48 h （[ 10.10 ± 0.36） h] of Gefitinib raw medicine group，Vz/F [（44.20±30.3）L/kg]，CLz/F[（1.89± 1.56） L/（h·kg）]，MRT0-48 h （[ 16.20 ± 2.52） h] of Gefitinib emulsion group were increased significantly （P＜0.05） AUC0-48 h （[ 38.70±26.20）mg·h/L] was decreased significantly （P＜0.05），and tmax （[ 10.40±3.25）h] was increased，without statistical significance. CONCLUSIONS： Compared with Gefitinib raw medicine，single and multiple administration of Gefitinib emulsion can effectively prolong the peak time，the results of this study can provide reference for new delivery system study of Gefitinib.
RESUMO
We investigated the epidemiological characteristics and temporal-spatial clustering of pulmonary tuberculosis (PTB),and analyzed its social influence factors in immigration city.Descriptive epidemiological analysis was conducted on PTB cases data extracted from the National Disease Reporting System of Chinese Center for Disease Control and Prevention between 2013 and 2015.Kulldorff scan statistics was applied to community based and town-based incidence data by SaTScan 9.1.1.The results were visualized by ArcMap10.2.A total of 12 109 PTB cases were reported in Ningbo with a decreasing trend in incidence rate and an increasing trend in proportion of floating population from 2013 to 2015.The male to female ratio was 2.07 ∶ 1 (8 162/3 947).All age groups were affected by PTB,but the incidence in 15-34 years age group and 65+ years age group were higher.Patients aged between 15 and 54 years accounted for 73.95 ％ of all cases.Most cases were farmers,housekeepers or unemployed,migrants and workers.There were six temporal-spatial clusters,of which the most likely clusters were in downtown and its surrounding areas,and the second likely clusters were in industrial parks.Compared with non cluster areas,proportion of floating population (t =2.88,P=0.01) especially immigrants from other provinces (t=7.46,P =0.00),and population density (t=3.37,P=0.00) in cluster areas were higher,while per capita green area was lower (t =-2.39,P 0.03).The downtown and its surrounding areas,industrial parks could be the future PTB combating regions.High population density and immigrants are associated with PTB clustering.
RESUMO
We investigated the epidemiological characteristics and temporal-spatial clustering of pulmonary tuberculosis (PTB),and analyzed its social influence factors in immigration city.Descriptive epidemiological analysis was conducted on PTB cases data extracted from the National Disease Reporting System of Chinese Center for Disease Control and Prevention between 2013 and 2015.Kulldorff scan statistics was applied to community based and town-based incidence data by SaTScan 9.1.1.The results were visualized by ArcMap10.2.A total of 12 109 PTB cases were reported in Ningbo with a decreasing trend in incidence rate and an increasing trend in proportion of floating population from 2013 to 2015.The male to female ratio was 2.07 ∶ 1 (8 162/3 947).All age groups were affected by PTB,but the incidence in 15-34 years age group and 65+ years age group were higher.Patients aged between 15 and 54 years accounted for 73.95 ％ of all cases.Most cases were farmers,housekeepers or unemployed,migrants and workers.There were six temporal-spatial clusters,of which the most likely clusters were in downtown and its surrounding areas,and the second likely clusters were in industrial parks.Compared with non cluster areas,proportion of floating population (t =2.88,P=0.01) especially immigrants from other provinces (t=7.46,P =0.00),and population density (t=3.37,P=0.00) in cluster areas were higher,while per capita green area was lower (t =-2.39,P 0.03).The downtown and its surrounding areas,industrial parks could be the future PTB combating regions.High population density and immigrants are associated with PTB clustering.