RESUMO
Objective To compare different disinfection methods for skin and bottle caps on the detection rate of coagulase negative staphylococcus(CNS)from blood culture,and provide basis for improving collection process and accuracy of blood culture.Methods Blood specimens from the neonatal department of a hospital between June 2012 and August 2014 were collected,blood specimens cultured between June 2012 and June 2013 were as control group (routine disinfection during blood collection process),and blood specimens cultured between August 2013 and Au-gust 2014 were as intervention group (improved disinfection method for skin and bottle caps),detection rates of CNS between two groups were compared.Results In control group,41 isolates of pathogens were isolated from 543 blood culture specimens,positive rate was 7.55%;in intervention group,21 isolates of pathogens were isolated from 853 blood culture specimens,positive rate was 2.46%.The detection rate of CNS in control group was 4.97%(n=27),12 (2.21 %)of which were methicillin-resistant coagulase-negative staphylococcus (MRCNS);the detec-tion rate of CNS in intervention group was 0.82%(n=7),6 (0.70%)of which were MRCNS.Difference in detec-tion rates of CNS and MRCNS between two groups were both statistically significant(χ2 =24.07,5.92,both P <0.05).Conclusion Improving disinfection methods for skin and caps of blood culture bottles during blood collection can decrease the detection rate of CNS.