Urogenital anomalies associated with anorectal malformations in children.

The urogenital tract is the most severe and common site of associated defects in anorectal malformations (ARM). Urogenital anomalies and their complications significantly increase the morbidity in these children after the ARM is corrected. The purpose of this study was to estimate the incidence of different types of urogenital anomalies with various types of anorectal malformations and to discuss the possible management and outcome. A retrospective study of patients with ARM and associated urogenital abnormalities admitted in the department of paediatric surgery of Sylhet MAG Osmani Medical College Hospital between 1998 and 2002 was undertaken. A total of 155 patients with Anorectal malformations were admitted of which 47 (30.32%) patients had associated anomalies involving other systems. Urogenital abnormalities were found in 25 (16.13%) patients. Patients of Anorectal malformations with urogenital abnormalities require careful assessment and well-timed intervention to minimize the morbidity.

Histological and histochemical study of endometrium in dysfunctional uterine haemorrhage.

Menstrual disorder is one of the most frequently encountered and perplexing conditions in adult women. Dysfunctional uterine haemorrhage (DUH) includes scanty, excessive or irregular bleeding, the primary cause of which is yet to become clear. A total of 100 cases of DUH were studied by histological and histochemical evaluation of endomertium with an object to find out the pathophysiology of DUH. Twenty cases of the similar age group without DUH acted as control. Irregular excessive bleeding was found to be the highest in multiparous women in their 4th decade of life. The material from endometrium was studied by haematoxylin and eosin statin, alcian blue, periodic acid Schiff's reaction and alkaline phosphatase activity. Histological examination of the study group revealed normal endometrium in 66% of cases and abnormal in 34% cases while histochemical behaviour was normal in 54% cases and abnormal in 46% cases. Hormonal imbalance is the chief factor in the pathogenesis of DUH and this abnormal hormonal status can better be revealed by a combined study of histological and histochemical evaluation of endometrium.

Maternal malnutrition, perinatal mortality and foetal pathology--a clinicopathological study.

The close relationship between the maternal malnutrition and consequent birth of low birth weight babies and the perinatal mortality is now an accepted fact and has been studied extensively in developed countries, though the problem is more acute in developing countries. The present study was proposed to find out relationship between the maternal nutritional status and the perinatal mortality. Autopsy was performed on 100 babies dying perinatally and pathological examination of different foetal organs in both well and malnourished mothers was also undertaken. Maternal nutritional status was evaluated by estimating haemoglobin level and total serum protein level. A mother with haemoglobin level below 11 g/dl and/or serum protein level below 5 g/dl was considered as malnourished. Pathological findings in the dead babies included low birth weight (500-1,999g), haemorrhage in multiple organs, atelectasis of the lungs, fatty changes in the liver, cystic changes of the kidneys and ill developed parenchyma of the pituitary.

Incidence of malignancy in Bankura (a retrospective study).

A retrospective study on pattern of malignant lesions in a rural district of West Bengal from 1981-1990 was conducted in the department of pathology, BS Medical College, Bankura, West Bengal. A total number of 17,130 cases were received out of which 1976 were malignant lesions (11.53%). The peak incidence of cancer in both sexes was found between the 4th, 5th and 6th decades, ie, 31-60 years of age. Sex-wise distribution showed more incidence in female (1246-62.17%) than in male (730-37.83%). Cancer of the cervix (35.62%) showed the highest incidence in this series. Cancer of the larynx (11.94%) was the commonest cancer in males. Malignancy of the lymph node was observed in 11.43% cases. Malignancy of the lungs and vagina (< 0.5%) showed very low incidence.

Frusemide-induced diabetes mellitus in insulin pretreated rabbits as an experimental model.

Single dose of frusemide 0.1 mg/kg orally was administered in 10 rabbits pretreated with soluble insulin 4 IU intravenously. Induction of diabetes after 24 h was confirmed by an increase in blood sugar and glucosylated haemoglobin and a decrease in serum insulin levels. Ten diabetic rabbits which received only frusemide without insulin pretreatment were used as controls, whereas equal number of insulin-pretreated rabbits receiving hydrochlorothiazide 250 mg/kg orally served as the positive control group. All biochemical parameters remained unaltered in the frusemide-treated test group as well as the positive control group after two weeks. The pancreatotoxic effect of frusemide may induce a stable hyperglycaemic state in rabbits which could lead to a useful diabetic model.

Hepatic lesions caused by excretory and secretory products of Ascaris lumbricoides in golden hamster.

OBJECTIVE: To ascertain the role of excretory and secretory (ES) products of Ascaris lumbricoides in liver damage. METHODS: The ES products of A lumbricoides were collected in vitro and their SDS-PAGE analysis was done. Feeding and subcutaneous injection of ES products were done in hamsters. Estimation of serum proteins, alkaline phosphatase and alanine aminotransferase and histology of liver were carried out. Control animal experiments were done concurrently. RESULTS: The ES products of A lumbricoides contained several proteins ranging in molecular weight from 14 to 205 Kd. Prolonged feeding of ES products caused elevation of ALT and amyloid deposition in the liver, whereas short term feeding or subcutaneous challenge caused focal cell necrosis and granuloma formation in the liver. CONCLUSION: ES products of A lumbricoides can produce liver damage.