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@#AIM: To evaluate the application effect of limbal stem cell transplantation fixed by corneal bandage lens with no suture method in primary pterygium excision surgery.<p>METHODS:Selected 25 patients of 50 eyes with bilateral primary pterygium who were admitted into our hospital from January 2019 to December 2019 for prospective clinical study. Group A(25 eyes)were randomly chosen with one eye of a patient fixed with corneal bandage lens with no suture; and Group B(25 eyes)were chosen with the other eye of a patient using traditional suture method. The incision healing, patient comfort, surgical complications, and postoperative recurrence were observed in both groups after postoperative follow-up for 6mo.<p>RESULTS: The average operating time for Group A(13.5±2.1min)was significantly less than that of Group B(26.6±7.2min). The results of postoperative follow-up in 1d, 1, 2wk, 1, 3 to 6mo showed that the discomfort such as pain, photophobia, lacrimation, foreign body sensation and itching were lower in Group A than in Group B. The discomfort disappeared in both groups after 6mo. The stability of postoperative corneal rim stem cell transplants was favorable in Group A, and the complications one and three months after surgery were less than those in Group B.<p>CONCLUSION: The method of banded corneal rim stem cell conjunctival transplantation combined with corneal bandage lens is an effective procedure for the treatment of primary pterygium. It is simple, convenient, safe and effective, the postoperative comfort is good, the recurrence rate is low, compared with the traditional suture method, it can shorten the operation time and effectively reduce the patient's pains.
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Objective To investigate the potential of neural stem cells (NSCs) injected into the cistema magna to migrate into the injured brain tissue to survive and differentiate into neural cells in rats with traumatic brain injury (TBI). Methods Embryonic NSCs cultured in vitro were labeled with BrdU and identified using immunofluorescence assay for nestin and BrdU expression. The labeled NSCs were stereotactically injected into the subarachnoid spaces of rats 24 h after experimental traumatic brain injury. The motor neurological function of the rats was assessed 24 h before and 24 h and 1 and 2 weeks after the injury, and immunohistochemistry was used to detect the expressions of BrdU, MAP2 and GFAP in the brain tissues. Results Positive expressions of nestin and BrdU were detected on the neurospheres by immunofluorescence assay. NSC transplantation resulted in significantly improved motor neurological function of the rats with traumatic brain injury (P<0.05). Immtmohistochemistry demonstrated the presence of BrdU-positive NSCs, MAP2-positive neurons and GFAP-positive glial cells in the traumatic brain tissue of rats 1 and 2 weeks after NSC transplantation. Conclusion Neural stem cells injected via the subarachnoid space can migrate into the injured rat brain tissue and differentiate into neural cells to promote the recovery of motor neurological function of rats with traumatic brain injury.