The predictive value of D-dimer combined with activated partial thromboplastin time for slow / no reflow during PCI in patients with acute coronary syndrome / 中国医师杂志

Objective:To analyze the predictive value of D-dimer combined with activated partial thromboplastin time (APTT) for slow / no reflow in patients with acute coronary syndrome (ACS) during percutaneous coronary intervention(PCI).Methods:From June 2017 to June 2019, 316 cases of patients with ACS who were to undergo PCI in Guigang People′s Hospital were selected as the study objects. The patients were divided into slow / no reflow group (SNR) and normal blood flow group (CON) according to the blood flow grading of thrombolysis in myocardial infarction (TIMI) during the operation. The differences of D-dimer and APTT between the two groups before operation were compared, and the predictive value of D-dimer combined with APTT for SNR was analyzed.Results:According to TIMI blood flow grading, all the patients were divided into SNR group ( n=71, 22.47%) and CON group ( n=245, 77.53%). The level of D-dimer in SNR group was significantly higher than that in CON group, while APTT was significantly lower than that in CON group ( P<0.05). Logistic regression analysis showed that D-dimer elevation ( OR=1.011, 95% CI: 1.008-1.015, P<0.001) was an independent risk factor of slow / no reflow in PCI for ACS patients, while APTT elevation ( OR=0.868, 95% CI: 0.818-0.921, P<0.001) was a protective factor. The D-dimer and APTT predicted that the area under receiver operating characteristic (ROC) curve of slow / no reflow in PCI were 0.814 and 0.738 respectively. The area under ROC curve of combined detection of D-dimer and APTT increased to 0.869, and the sensitivity and specificity were 87.3% and 75.1% respectively ( P<0.001). Conclusions:The D-dimer and APTT have a high predictive value of slow / no reflow during PCI in ACS patients. The combined detection of the two is helpful to identify the occurrence of slow / no reflow in PCI.

Association of inflammatory indices with the severity of urinary sepsis: analysis of 70 cases / 南方医科大学学报

OBJECTIVE@#To analyze the association of the clinical inflammatory indices with the severity of urinary sepsis.@*METHODS@#We reviewed the clinical data of 70 patients with urinary sepsis treated in our hospital between January, 2013 and April, 2018. All the patients were diagnosed in line with the Guidelines for Diagnosis and Treatment of Urological Diseases in China (2014 edition), including 22 patients with sepsis, 12 with hypotension and severe sepsis, 17 with septic shock, and 19 with critical septic shock. White blood cell count (WBC), neutrophil percentage (N%), platelets (PLT), fibrinogen (FIB), Ddimer, interleukin-6 (IL-6), procalcitonin (PCT) and C-reactive protein (CRP) were examined in all the cases and compared among the 4 groups. The correlations of these inflammatory markers with the severity of sepsis were analyzed using logistic regression analysis.@*RESULTS@#The 4 groups of patients showed significant differences in N%, PLT, D-dimer, and PCT ( < 0.05) but not in CRP (>0.05). Kruskal-Wallis Pairwise comparisons showed that the N% and PCT in patients with sepsis differed significantly from those in the other 3 groups; platelets in patients with sepsis differed significantly from those in patients with septic shock and critical septic shock; D-dimer differed significantly between patients with sepsis and those with septic shock. Among the 4 groups, the median levels of PLT decreased and PCT and N% increased with the worsening of sepsis. Logistic regression analysis indicated that PCT (=0.186, =0.000), N% (=0.047, =0.035) and PLT (=-0.012, =0.003) were significantly correlated with the severity of sepsis in these patients.@*CONCLUSIONS@#PCT, PLT and N% are all significantly correlated with the severity of sepsis, and their combined detection can be informative for assessing the severity of sepsis to facilitate clinical decisions on treatment.

In vitro culture medium for sparse spermatozoa improves human sperm motility / 中华男科学杂志

Objective@#To investigate whether in vitro culture medium (IVCM) for sparse spermatozoa can improve human sperm motility for the purpose of helping clinicians, laboratorians and patients choose a better strategy of assisted reproduction.@*METHODS@#Semen samples were obtained from 178 males for routine semen examination from March to August 2016, including 151 cases of asthenozoospermia and 27 cases of normal sperm motility. A total of 200 μl was collected from each sample and divided into two equal portions and equal volumes of IVCM (experimental group) and F10 (1×) (control group) were added to the two portions, respectively, followed by 30-minute incubation at 37℃ in an incubator with 5% CO2. Sperm concentration, motility and viability and the percentages of progressively motile, non-progressively motile and immotile sperm were recorded before and after incubation.@*RESULTS@#After activated with IVCM, neither the samples with asthenozoospermia nor those with normal sperm motility showed any statistically significant difference in sperm viability from the baseline or the control group (P>0.05). The rates of progressively and non-progressively motile sperm from the asthenozoospermia males were increased by 14.02% and 4.86% respectively, while that of immotile sperm decreased by 19.01% in the experimental group (P >0.01), and similar results were observed in the semen samples from the men with normal sperm motility. The percentage of reduced immotile viable sperm was positively correlated with that of immotile viable sperm in both the asthenozoospermia patients (r = 0.260, P <0.01) and the men with normal sperm motility (r = 0.679, P <0.01).@*CONCLUSIONS@#IVCM can increase sperm motility without affecting sperm viability in men with either asthenozoospermia or normal sperm motility. The larger the proportion of immotile viable sperm, the higher the percentages of progressively and non-progressively motile sperm in the semen after IVCM activation, and this correlation is more significant in men with normal sperm motility than in asthenozoospermia patients.

Incidence and risk factors of urinary tract infection after ureteroscopy / 中国感染控制杂志

Objective To evaluate the incidence and risk factors of urinary tract infection(UTI)following uret-eroscopy.Methods Patients undergoing ureteroscopy examination or ureteroscopic lithotripsy in a hospital between 2002 and 2011 were analyzed retrospectively,clinical data of patients were collected and analyzed,including age, sex,history,complication ,urine routine test,urine culture,blood routine test,urethral catheterization,ureteral stent placing and antimicrobial use.Results Incidence of UTI following ureteroscopy was 3.77% (20/531),UTI fol-lowing ureteroscopy examination was higher than ureteroscopic lithotripsy (5.84% [9/154]vs 2.92% [11/377]). Pyelonephritis was the main infection type(n= 15 ),the main pathogen was Escherichia coli (n= 6 ),there was no statistical difference in UTI among patients receiving different types of antimicrobial prophylaxis (P= 0.185 ). Patients with bacteriuria,hydronephrosis,urethral catheterization,without ureteral stent placing,and without re-ceiving antimicrobial prophylaxis had higher incidence of URI(all P<0.05).Conclusion Bacteriuria,hydronephro-sis,urethral catheterization,without postoperative ureteral stent placing,ureteroscopy examination,and without receiving antimicrobial prophylaxis are risk factors of UTI following ureteroscopy.

Analysis on 21 cases of chylous fistula after neck lymph nodes dissection / 重庆医学

Objective To discuss the prevention and management method for chylous fistula after neck lymph node dissec‐tion .Methods Totally 1 793 cases of neck lymph node dissection in this department from January 2005 to September 2014 were retrospectively analyzed .The clinical data in the cases of chylous fistula occurred after operation were summarized .Results Twenty one cases of chylous fistula occurred ,accounting for 1 .17% ,in which 13 cases were cured by the local compressed bandaging and continuous negative pressure drainage;5 cases adopted the conventional method for 2-3 d ,but under the ineffective condition ,then they were treated by combining with somatostatin pumping (somatostatin 6 mg+0 .9% normal saline 48 mL ,2 mL/h ,lasting for 24 h ,for successive 2-3 d) and finally cured;3 cases were cured after reoperation .Conclusion Prevention is the best treatment for chylous fistulas ,local compression bandage plus continuous negative pressure drainage is the main method for treatment of chylous fistulas after neck dissection .The combined therapy with somatostatin can increase the close rate of chylous fistulas;for the patients with long persistent time ,large drainage volume and invalid conservative therapy should adopt the remedial measure of operation .

The expression of thrombospondin 1 and its gene hypermethylation in laryngeal squamous cell cancer / 肿瘤

Objective: To explore the mRNA and protein expressions of thrombospondin 1 (THBS1) gene and the DNA methylation of its promoter in laryngeal squamous cell cancer (LSCC), and to analyze their relationship to clinicopathological features of LSCC. Methods: Sixty-six pairs of fresh LSCC tissues and the matching adjacent normal tissues were taken from 66 cases of LSCC. The mRNA and protein expressions of THBS1 gene were detected by revere transcription PCR and Western blotting assay, respectively. Then their relationship to clinicopathological features of LSCC was analyzed by one-way ANOVA and t test. The methylation of THBS1 gene promoter was detected by the DNA methylation specific PCR, and its relationship to clinicopathological features of LSCC was analyzed by ?2 test. Results: The mRNA and protein expressions of THBS1 gene in LSCC tissues were significantly decreased as compared with those in the adjacent normal tissues (P < 0.05), and these expressions were also associated with lymph node involvement and TNM staging (P < 0.05). The positive rate of the hypermethylation of THBS1 gene in the LSCC tissues (48.50%±6.15%) was significantly higher than that in the matching adjacent normal tissues (6.06%±2.92%) (P < 0.05). The mRNA and protein expressions of THBS1 gene were associated with the hypermethylation of its gene promoter (P < 0.05). THBS1 gene hypermethylation status in LSCC tissues was associated with lymph node involvement and TNM staging (P < 0.05). Conclusion: The decreased expressions of THBS1 mRNA and protein and the high methylation of its gene promoter may play crucial roles in carcinogenesis and development of LSCC. Copyright © 2014 by TUMOR.

The clinical application research on the island pedicled pectoralis major muscle flap in repairing of the tissue defects after the operation of head and neck malignant tumors / 重庆医学

Objective To investigate the primary repair methods and efficacy of island pedicled pectoralis major muscle flap in soft tissue defect after the operation of head and neck malignant tumors .Methods From April 2011 to September 2012 ,27 patients from this hospital who underwent primary repair using island pedicled pectoralis major muscle flap after a head and neck extensive soft tissue defect were investigated ,including 11 cases of tongue cancer defect ,2 cases of parotid gland defect ,3 cases of gum cancer defect ,2 cases of oropharyngeal cancer defect ,9 cases of floor of the mouth cancer defect .Results 27 patients were safety in periop-erative ,27 cases of island pectoralis major muscle flap survived ,patients were satisfied with both the appearance and function .There was postoperative neck hematoma in 1 patient ,it was eliminated through timely remove the hematoma and hemostasis completely ;There was 1 case of removing the epidermal necrolysis skin as flap necrosis ,while it was acceptable efficacy as the muscle flap sur-vival and surface granulation repaired .Conclusion Island pectoralis major muscle flap obtains unique advantages in repairing a large area of soft tissue defect in head and neck cancer patients after operation ,such as abundant of tissue ,a constant arterial anatomy of thoracoacromial ,easy to operate ,rich blood supply ,strong resistance to infection ,high survival rate and so on .A fine surgery opera-tion is the key of island pectoralis major muscle flap survival .

Expression and clinicopathological significance of NF? B p65 and PTEN in laryngeal squamous cell carcinoma / 重庆医科大学学报

Objective:To investigate the expression of NF?B p65 and PTEN in laryngeal squamous cell carcinoma(LSCC) and their clinical significance.Methods:Immunohistochemical staining was used to examine the expression of NF?B p65 and PTEN in 52 cases of LSCC and 30 cases of adjacent normal tissues.Results:The expression positive rates of NF?B p65 in LSCC were obviously higher than those in adjacent normal tissues(P0.05).The Spearman analysis indicated that the expression level of NF?B p65 was negatively correlated with that of PTEN significantly(rs=-0.580,P

Clinical observation of treating allergic rhinitis with focused ultrasound under nasal endoscope / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To observe the short-term therapeutic effect of focused ultrasound in treating allergic rhinitis under nasal endoscope.@*METHOD@#One hundred and seventeen patients with allergic rhinitis were treated by the CZB ultrasound therapeutic system which was developed and produced by Chongqing Haifu (HIFU) Technology Co. Ltd., Chongqing China. It was used to scan inferior nasal concha and the anterosuperior part of nasal septum under nasal endoscopy. The therapeutic effect was evaluated by clinical symptoms controlled counting-scores, according to the Chinese Lan zhou principle of treatment and recommendation program for allergic rhinitis.@*RESULT@#The excellent effective rate was 44/117 cases (37.6%), effective rate was 49/117 cases (41.9%), ineffective rate was 24/117 cases (20.5%), and the total effective rate was 79.5 after 12 months follow up.@*CONCLUSION@#The focused ultrasound is convenient, safe, repetitive and effective in treating allergic rhinitis under nasal endoscope. The long-term therapeutic effect remains to be further studied.