RESUMO
Objective:To investigate gene basis of primary open angle glaucoma (POAG) by comparing gene expression profile of trabecular meshwork between POAG patients and normal controls by using RNA-sequencing.Methods:Trabecular meshwork specimen were obtained from trabeculectomy (POAG group, n=3) or donated eyes (control group, n=2). RNA was extracted and sequenced in both groups, gene expression profiles were analyzed and compared between them, and different expression genes associated with POAG were revealed by using Database for Annotation, Visualization and Integrated Discovery (DAVID) and Protein Analysis Through Evolutionary Relationships (PANTHER) gene list analysis.Written informed consent was obtained from each patient or the family members prior to entering the study cohort.The study protocol was approved by the Ethics Committee of Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong [No.EC20140311(3)-P01]. Results:(1)Total of 28 821 genes were obtained from RNA-sequencing, 22 genes were statistically significant between the two groups, of which one gene was up-regulated and 21 genes were down-regulated; (2)Genes that expressed differently had concentrated functions, biological process involved keratinization, epidermis development and intermediate filament cytoskeleton organization, cellular component related to keratin filament, intermediate filament, extracellular exosome and haptoglobin-hemoglobin complex, molecular function related to structural molecule activity and structural constituent of cytoskeleton; (3)Significantly enriched PANTHER pathways were plasminogen activating cascade, p38 MAPK pathway, oxidative stress response and p53 pathway.Conclusions:Trabecular meshwork and extracellular matrix remodeling due to abnormal keratin expression, structural change of intermediate filament cytoskeleton and misregulation of plasminogen activating cascade, p38 MAPK pathway were possible etiology of POAG.Differential expressed genes that related to POAG mainly involve cytoskeleton associated genes and extracellular matrix remodeling genes.Thus, regulation of these genes may have an effect on glaucomatous treatment.
RESUMO
Objective:To investigate the association of ocular dominance with the severity of chronic primary angle-closure glaucoma (PACG).Methods:Ocular dominance was assessed via the " hole in card" method.The anatomical symmetry (including anterior chamber depth, lens thickness and axial length) in both eyes was analyzed via A scan ultrasound.The severely glaucomatous eye was determined by the mean defect of visual field.The association of ocular dominance with the severity of chronic PACG was then analyzed.This study followed the Declaration of Helsinki, and the study protocol was approved by the Ethics Committee of Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong.Written informed consent was obtained from all subjects prior to their entering the study cohort.Results:Visual acuity (LogMAR) was 0.39±0.24 in the dominant eye group, and 0.43±0.29 in the non-dominant eye group.Anterior chamber depth was (2.53±0.26)mm in the dominant eye group, and (2.54±0.29)mm in the non-dominant eye group.Lens thickness was (4.96±0.31)mm in the dominant eye group, and (4.92±0.33)mm in the non-dominant eye group.Axial length was (22.58±0.61)mm in the dominant eye group, and (22.73±1.11)mm in the non-dominant eye group.No significant difference was found in visual acuity, anterior chamber depth, lens thickness or axial length between the dominant and non-dominant eye groups ( t=-1.643, -0.797, 1.867, -1.345; all at P>0.05). The vertical cup-disc ratio of the dominant eye group was lower than that of the non-dominant eye group (0.55 [0.40, 0.80] vs. 0.80 [0.63, 0.90]). The mean defect in the visual field of the dominant eye group was lower than that in the non-dominant eye group (-6.54 [-16.70, -3.85]dB vs.-18.77 [-28.19, -8.55]dB), and the intraocular pressure in the dominant eye group was lower than that in the non-dominant eye group (21.00 [17.00, 27.75]mmHg vs. 24.50 [19.00, 36.25]mmHg) (1 mmHg=0.133 kPa). Significant differences were found in mean defect, vertical cup-disc ratio and intraocular pressure between the two groups ( Z=-3.781, -3.528, -2.126; all at P<0.05). The ratio of the severely glaucomatous eye being the non-dominant eye was 84.09%, which was much higher than that of the severely glaucomatous eye being the dominant eye (15.91%). The non-dominant eye was related to the severity of chronic PACG ( χ2=40.909, P<0.001, Pearson contingency coefficient r=0.563). Conclusions:The non-dominant eye is associated with the severity of chronic PACG.
RESUMO
Objective To evaluate the effectiveness and safety of modified largeincision manual cataract extraction combined with intraocular lens (IOL) implantation for high myopia with cataract.Methods Twenty-eight patients (32 eyes) of high myopia with cataract underwent modified large-incision manual cataract extraction combined with IOL implantation.The nuclear hardness of 20 eyes were in degree Ⅲ,11 eyes were in degree ⅣV,1 eye was in degree V.The follow-up time was form 2 months to 12 months,the visual acuity,refraction and complications were observed and analyzed,Resuits In 32 eyes,the postoperative best corrected visual acuity (BCVA) was better than pre-operation in 28 eyes,4 eyes had no any improvement,in which the server high myopic fundus disease was found.The preoperative and postoperative BCVA were (1.43 ± 0.73) IogMAR and (0.84 ± 0.71) logMAR,and the sphere equivalent were (-15.33 ± 5.03) D and (-1.86 ± 1.41) D,the differences were statistically significant (all P ＜ 0.05).The intraoperative partial suspensory ligament occurred in 1 eye,iris hemorrhage appeared in 1 eye when extracting the lens nucleus;The postoperative mild corneal edema was seen in 11 eyes,hyphema in 1 eye,temporary high IOP in 1 eye,uveitis in 3 eyes,which disappeared within 1 week after treatment.IOL were implanted into capsular bag except 1 eye for partly broken ciliary zonule.There was no IOL dislocation within the follow-up.Conclusion Modified large-incision manual cataract extraction combined with IOL implantation is safe and effective for high myopia with cataract.
RESUMO
Background The establishment of chronic ocular hypertension is a basis for the research of glaucoma.Previous laser photocoagulation method to establish ocular hypertension model showed obvious fluctuation of intraocular pressure (IOP) and complications and need repeatedly photocoagulation.Improvement of modeling method is of important significance for glaucoma.Objective This study was to establish chronic ocular hypertension rat models by transgoniscope laser photocoagulation to trabecular meshwork and to compare this method with previous translimbal laser photocoagulation.Methods Thirty-six 8 to 12-week-old clean grade Fischer344 rats were collected and divided into normal control group,translimbal laser photocoagulation group and transgoniscope laser photocoagulation group,12 rats for each group.Five hundred and thirty-two nm YAG laser was used to photocoagulate trabecular meshwork translimbally in the right eyes of rats in the translimbal laser photocoagulation group,with the laser power 440-500 mW and spots 40-60,and the photocoagulation was perfored transgoniscopely in the right eyes of rats in the transgoniscope laser photocoagulation group,with the laser power 800-850 mW and spots 100-120.IOP was measured by using Tonolab tonometer in all the rats after modeling.The rats were sacrificed 3 weeks after modeling and retinas were isolated,the Tuj-1 positive retinal ganglion cells (RGCs) were counted by immunofluorescence technology.The use and care of the animals followed the Statement of ARVO.Results The successful rate of establishement of models was 75％ in the translimbal laser photocoagulation group and 100％ in the transgoniscope laser photocoagulation group.The mean IOP was (11.0±1.3),(23.4±12.6) and (25.3± 4.9) mmHg,and the peak IOP was (12.3 ± 1.0),(50.5 ± 7.3) and (44.3 ± 12.3) mmHg in the normal control group,transgoniscope laser photocoagulation group and translimbal laser photocoagulation group,respectively,with significant differences among the groups (F=25.496,80.762,both at P＜0.001),and the mean IOP was significantly higher in the transgoniscope laser photocoagulation group and translimbal laser photocoagulation group than that in the normal control group (all at P＜0.001),and no significant differences in the mean and peak IOP between transgoniscope laser photocoagulation group and translimbal laser photocoagulation group (P=1.000,P=0.195).The numbers of Tuj-1 positive RGCs in the retinas were (2 048.2± 148.5),(645.2 ± 177.1) and (1 223.7 ± 148.6)/mm2 in the normal control group,transgoniscope laser photocoagulation group and translimbal laser photocoagulation group,showing a significant difference among the groups (F=98.767,P＜0.001).The number of Tuj-1 positive RGCs was considerably reduced in the transgoniscope laser photocoagulation group and translimbal laser photocoagulation group compared with the normal control group and the number of Tuj-1 postive RGCs was low in the translimbal laser photocoagulation group compared with the transgoniscope laser photocoagulation group (all at P＜0.001).Conclusions Transgoniscope laser photocoagulation targeting trabecular meshwork can induce chronic ocular hypertension and RGCs losing.However,its pattern is different from translimbal laser photocoagulation.Transgoniscope laser photocoagulation has a higher successful rate of chronic ocular hypertention than that of translimbal laser photocoagulation.
RESUMO
Background Reliable intraocular pressure (IOP) measurement is essential in glaucomatous study.TonoLab is a newly designed tonometer for small animals,however,its repeatability and reproducibility remains unknown.Objective This study aimed to evaluate the repeatability and reproducibility of TonoLab rebound tonometer in DBA/2J mice.Methods Intraocular pressure was measured in both eyes of 63 DBA/2J mice aged 7 months under systemic anesthesia with TonoLab rebound tonometer.IOP of each eye was measured for 3 times by two observers independently and consecutively using TonoLab rebound tonometer,and the data were obtained in single blind method.Within standard deviation (Sw),interclass correlation coefficient (ICC),within coefficient of variation (CVw) and corresponding 95％ confidence interval (CI) were calculated to evaluate the repeatability of measuring result from single observer,and Bland-Altman method was used to assess the agreement between two observers.The abnormal data which affected repeatability was analyzed.Results Among the 126 eyes,105 eyes showed the normal IOP with mean value (12.98 ± 1.05) mmHg from observer 1 and (12.41 ±0.92) mmHg from observer 2.Ocular hypertension was found in 21 eyes with mean value (27.76 ± 1.95) mmHg and (27.49 ± 1.28) mmHg for observer 1 and observer 2 respectively.Sw,ICC and CVw for observer 1/observer 2 were 1.34/1.11 mmHg,0.90/0.92,8.24％/7.42％ in normal eyes and 2.21/1.49 mmHg,0.89/0.95,6.97％/4.82％ in hypertensive eyes.The average bias between two measuring values from different observers was 0.60 mmHg in the normal eyes (95％ CI:-5.20-6.30 mmHg) and 0.30 mmHg in the hypertensive eyes (95％ CI:-7.30-7.80 mmHg).Conclusions TonoLab rebound tonometer can offer a good intraobserver repeatability in both normal and hypertensive eyes in mice,but the agreement between two observers is outside an acceptable level.So we consider IOP in mice obtained by TonoLab rebound tonometer is reliable and accurate from a single observer.