Effect of ubiquitin-specific protease on radiosensitivity of tumor / 国际肿瘤学杂志

Ubiquitin-specific protease is a member of the deubiquitinating enzyme family,which can reverse the ubiquitination of proteins and stabilize proteins. It affects radiosensitivity of tumor by regulating DNA damage,cell cycle,nuclear factor-κB signaling pathway,Nrf2-Keap1 signaling pathway and oncogene c-myc. It is expected to become a new target for tumor radiotherapy sensitization.

The dominant immunoglobulin G1 expression in the salivary glands of Sj?gren's syndrome correlates with pathological injury / 中华风湿病学杂志

Objective The purpose of this study was to clarify the distribution of immunoglobulin (Ig) G subclasses in the salivary glands of Sj?gren's syndrome (SS). Methods The salivary glands from 70 patients were stained with IgG and IgG subclasses monoclonal antibodies. Clinical data was collected and analyzed between different groups. For group comparisons,measurement data were compared using Student's t test and Mann-Whitney U test, while count data were measured using chi-square test. Results All four IgG subclasses could be detected. IgG could be detected in 36 patients (51％), IgG1 in 32 patients (46％), IgG2 in 3 patients (4％), IgG3 in 5 patients (7％), and IgG4 in 6 patients (9％). IgG1 positive group appeared to have longer duration [(74±75) months (25±36) months, t=3.57, P<0.05), lower white blood cell count (WBC) [(5.5±2.5)×109/L vs (7.3±3.6)×109/L, t=2.38, P<0.05), higher positive rate for antinuclear antibody (ANA), anti-SSA, anti-SSB (97％ and 74％, 93％ and 65％, 47％ and 15％, χ2 was 6.08, 7.46, 8.22 respectively, P<0.05) and more Chisholm 3/4 pathological grade cases (97％与 67％, χ2=9.22), They were all stastically significant (P<0.05). Conclusion IgG1 is the main IgG subclass in the salivary glands of SS. IgG1 may take part in the immunological injury of salivary glands destruction in SS;The dominant IgG1 expression in the salivary glands of SS correlates with pathological injury.

Effects of Rehabilitation Nursing Intervention on Quality of Life of Burn Patients:A Meta Analysis / 中国康复理论与实践

Objective To investigate the effects of rehabilitation nursing on quality of life in patients after burn injuries. Methods The re-ports about rehabilitation nursing for burn patients from January, 2004 to June, 2015 were searched and extracted from the databases of Chi-na Biology Medicine, China National Knowledge Infrastructure, Wangfang Data and Chongqing VIP Database, and analyzed with Review Manager 5.3. Results 11 non-randomized control studies (MINROS score:13~19) were eligible for meta-analysis. There were significant differences in the general health (MD=7.78, 95%CI:6.79~8.77, P<0.001), social function (MD=10.26, 95%CI:5.91~14.60, P<0.001), men-tal function (MD=9.71, 95%CI:6.87~12.55, P<0.001) and physical function (MD=6.88, 95%CI:3.95~9.81, P<0.001) between experimental group and control group. Conclusion Rehabilitation nursing can improve the quality of life of burn patients.

Study on the relationship between semen leukocyte and liquefaction and sperm motility / 国际检验医学杂志

Objective To explore the relationship between semen leukocyte and liquefaction and sperm motility through the anal‐ysis of routine semen examination results .Methods Retrospectively analyze on 8 666 cases of routine semen examination .Accord‐ing to the leukocyte count the semen samples which had sperms were divided into three groups ,Group A :white blood cells≤1 × 106/mL ,Group B:(1-4)× 106/mL ,Group C :>4 × 106/mL .Then compare the liquefaction time and sperm motility of these three groups .Results Among the 8 666 cases of semen analysis ,there were 164 cases of azoospermia(accounting for 1 .9% ) and 8 502 fine cases(accounting for 98 .1% ) .There were 7 419 ,1 014 and 69 cases in Group A ,B ,C respectively .In the three groups ,there were 5 323 ,740 and 50 cases of normal sperm motility respectively ;there were 2 096 ,274 and 19 cases of the abnormal motility ca‐ses respectively .In the three groups ,the normal cases of semen liquefaction time were 4 593 ,608 and 43 respectively ;the abnormal were 2 826 ,406 and 26 cases respectively .Statistical analysis showed no significant correlation between semen leukocyte and lique‐faction(P=0 .712) ,and between semen leukocyte and sperm motility(P=0 .486) .There were 1 217 cases of normal sperm motility and 4 027 cases of abnormal sperm motility in normal liquefaction group;there were 1 172 cases of normal sperm motility and 2 086 cases of sperm motility in abnormal liquefied group .There was statistically significant difference between the two groups(P=0 .000<0 .05) .Conclusion There were no correlation between semen leukocyte count and liquefaction or sperm motility ,but sperm mo‐tility and liquefaction are correlated .

Expression of IgG4 in thyroid tissues of Hashimoto's thyroiditis and its clinical and histological characteristics / 中华内分泌代谢杂志

Immunohistochemistry was carried out for assessment of the expression of IgG4 and IgG in 29 patients with Hashimoto's thyroiditis (HT) and 26 other patients with subacute thyroiditis,thyroid adenoma,and thyroid carcinoma.HT group were divided into IgG4 HT (10/29) and non-IgG4 HT (19/29) groups.Statistical analyses of clinical and histological characteristics were also conducted in both groups.2 cases of subacute thyroiditis were IgG4 positive (2/26).The IgG4 positive plasma cells,IgG positive plasma cells,and ratio of IgG4 +/IgG + plasma cells in IgG4 HT cases were significantly higher than non-IgG4 HT ones(P＜0.05).The serum Fl4 in IgG4 HT cases were lower than non-IgG4 HT ones(P＜0.05).After operation,IgG4 HT had a greater proportion for accepting thyroxin treatment(P＜0.05).IgG4 HT is a new-found disease which has particular histological and immunological features and would have good response for cortical hormone therapy.Serum and tissue examination of IgG4 seems to be helpful to the diagnosis of IgG4 HT.

The expression of IgG4 in salivary glands and the clinical analysis of 58 cases of Sj(o)gren's syndrome / 中华风湿病学杂志

Objective Mikulicz's disease (MD) was considered to be a subtype of Sj(o)gren's syndrome (SS) due to the clinical and histological similarities between them.Evidence had shown that there were differences between MD and typical SS.The purpose of this study was to investigate the correlation between MD and SS,by means of analyzing the expression of IgG4 in salivary glands and the clinical characteristics of patients who were previously considered as SS.Methods The paraffin sections of salivary glands from SS patients were stained with monoclonal antibodies to IgG4 and CD38.Patients were divided into two groups based on the pathological results.Analysis of the symptoms,the signs and the laboratory results were carried out in these patients.The difference in laboratory parameters and histopathological gradings in the two groups was analyzed.Normal and abnormal distributed data comparison was conducted using random independent samples t-test and rank sum test respectively.Two-sample rates were compared with Chi-square test.Results Based on immunohistochemistry of IgG4 distribution,the 58 patients with SS were divided into two groups:IgG4 related (9 cases) and non-IgG4 related (49 cases).Histopat-hologically,IgG4 related cases showed IgG4+ plasma cells/IgG+ plasma cells infiltration and there were more IgG4 related monoclonal antibody expressed when compared to IgG4 unrelated cases.In addition,there were also significant differences in clinical features between the two groups.IgG4 related disease was associated with male gender,higher level of plateletconnt,lymphocytes [(2.4±0.8)×109/L vs (1.4±0.7) ×109/L] count and CRP [(52±60) mg/L vs (15±17) mg/L] levels and lower titer of IgM [(1.2±0.7) g/L vs (1.8±0.8) g/L],antinuclear antibody (56％ vs 87％) and anti-SSB antibodies (13％ vs 54％) (P＜0.05),when compared with IgG4-unrelated cases.There was no significant difference in other indicators (P＞0.05).Conclusion The present study has demonstrated that some of the MD patients misdiagnosed as SS.Some of the laboratory tests such as the level of platelet and lymphocyte count,serum level of CRP,IgM,antinuclear antibody,anti-SSB antibodies,the serum levels of IgG4 and the histopathological presentations in the salivary gland are different between these two disorders.Because of good response to steroid in MD,so laboratory tests and pathological examinations for IgG4 can help to avoid misdiagnosis.

Factors Related with Activity of Daily Living and Rehabilitation Nursing for Fractures for Patients Injured in Earthquake / 中国康复理论与实践

@# ObjectiveTo explore the factors associated with activity of daily living (ADL) in patients injured from earthquake and to observe the rehabilitation nursing of fractures for them. Methods367 patients were reported and analyzed with Logistic regression. Results and ConclusionThe age (OR=1.016), renal failure (OR=4.648), lower limb fractures (OR=2.404), spine fracture (OR=3.558), right hand fractures (OR=1.190), right foot fractures (OR=4.389) and the complex fractures (OR=2.600) were associated with the poor ADL (<60). The rehabilitation nursing for fracture were reported and discussed.

Diagnostic accuracy of cell-block or tissue-fragment histology and cytology by fine needle lung aspiration / 中华病理学杂志

<p><b>OBJECTIVE</b>Study on the diagnostic accuracy and value of cell block and tissue fragment preparations collected from lung fine needle aspiration (FNA).</p><p><b>METHODS</b>A total of 187 FNA (22G) samples from the lungs with matched histological diagnosis were studied. Among them, the diagnosis made by depending on 124 cell block and fragment preparations were analyzed in comparing retrospectively with the diagnosis of 187 cases by smear preparations.</p><p><b>RESULTS</b>(1) Of the 124 cell blocks cases, 89 cases were true positives, 22 cases were true negatives, 13 cases were false negatives and no false positives. Of the 187 smears cases, the figure were 136, 30, 19 and 2 cases respectively. The diagnostic accuracy of cell blocks was 87.3% in sensitivity, 100% in specificity, 89.5% in overall accuracy. The figures for smears were 87.7%, 93.8% and 88.8% respectively. (2) For malignant tumours, the histological typing accuracy of cell blocks was 93.3% (83/89), and to be 67.9% (91/134) by diagnosis depending on the smears (P < 0.01). For the benign lesions, the figures were 86.4% (19/22) and 60% (18/30) respectively (P < 0.05). (3) It was possible to obtain many minisections for further studies from cell blocks. Immunoperoxidase staining on minisections was reliable and agreed with those on the surgical specimens.</p><p><b>CONCLUSIONS</b>The diagnostic accuracy of cell block is high, particularly in histological typing which approaches to that of the diagnosis made depending on the postoperative specimens. A combined use of smears and cell block is recommended which may raise further the diagnostic accuracy.</p>