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Objective To explore the roles of CD34,VEGF and endothelin-1 in hepatic sinusoid capillarization and the intrahepatic metastasis of hepatocellular carcinoma.Methods Data of 10 cases of hepatocellular carcinoma without intrahepatic metastasis were compared with those 10 cases of hepatocellular carcinoma with intrahepatic metastasis.Liver cancer tissues and paracancerous tissues were collected,hepatic sinusoidal capillarization was detected by immunohistochemistry,mRNA expression and protein expression of CD34,VEGF and endothelin-1 genes were detected by QPCR,Western blot,and the relationship between gene expression and metastasis of HCC was observed.Results Hepatic sinusoid capillarization was observed in carcinoma tissues,not in the adjacent tissues,whereas mRNAs and proteins of CD34,VEGF and endothelin-1 were higher in liver cancer tissues than that in the adjacent tissuess.In addition,both mRNA and protein expression levels of these genes were significantly higher in the intrahepatic metastatic liver cancers than those without metastasis (P ＜ 0.05).Conclusion CD34,VEGF and endothelin-1 may play important roles in intrahepatic metastasis of liver cancer.
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Objective@#To explore the roles of CD34, VEGF and endothelin-1 in hepatic sinusoid capillarization and the intrahepatic metastasis of hepatocellular carcinoma.@*Methods@#Data of 10 cases of hepatocellular carcinoma without intrahepatic metastasis were compared with those 10 cases of hepatocellular carcinoma with intrahepatic metastasis . Liver cancer tissues and paracancerous tissues were collected, hepatic sinusoidal capillarization was detected by immunohistochemistry, mRNA expression and protein expression of CD34, VEGF and endothelin-1 genes were detected by QPCR, Western blot, and the relationship between gene expression and metastasis of HCC was observed.@*Results@#Hepatic sinusoid capillarization was observed in carcinoma tissues, not in the adjacent tissues, whereas mRNAs and proteins of CD34, VEGF and endothelin-1 were higher in liver cancer tissues than that in the adjacent tissuess. In addition, both mRNA and protein expression levels of these genes were significantly higher in the intrahepatic metastatic liver cancers than those without metastasis (P<0.05).@*Conclusion@#CD34, VEGF and endothelin - 1 may play important roles in intrahepatic metastasis of liver cancer.
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Objective:To detect the activity of IgY against complex bacteria in pharynx and throat.Methods:Purified antigens against bacteria in pharynx and throat was used to immunize egglaid hens.The eggs from immunized hens were collected and abstract IgY from the yolks.The antibody activity of IgY was detected by SDS-PAGE electrophoresis and ELISA.Results:SDS-PAGE electrophoresis represented at least twelve ladders,and the titer of ELISA was 1∶512.Conclusion:IgY antibody was obtained in egg yolk after immunized hens with complex bacteria.The activity of IgY was detected.IgY showed stable to heat.