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objective To investigate the protective effect and possible mechanisms of extract of Gingko biloba(EGB)on early diabetic nephropathy(DN).Methods Fifty-one patients with early DN were randomly divided into control group(n=25)treated by irbesartan alone and treated group(n=26)treated by EGB combined with irbesartan.Serum high sensitive C-reactive protein(hs-CRP)and monocyte chemoattractant protein-1(MCP-1)were determined with ELISA before and after treatment,and urinary albumin excretion rate(UAER),fasting blood glucose (FBG),serum creatinine(SCr),blood urea nitrogen(BUN)and lipid profiles were examined as well.Results Compared with pretreatment,at the end of 6 months'treatment,the values of hs-CRP decreased from(194.37±34.02)and(191.51±27.15)mg/L to(164.13±32.86)and(134.65±20.47)mg/L in control group and treatment group,respectively.MCP-1 was downregulated from(304.23±38.56)and(299.66±44.07)ng/L to (235.43±28.66)and(165.53±21.96)ng/L in control group and treated group(all P＜0.05).After treatment,the decrement of hs-CRP and MCP-1 level in the treated group was more significant than that in control group(P＜0.05).Conclusion EGB could retard the development of early DN,through down-regulating the expresssion of seram MCP-1,decreasing serum hs-CRP concentration and inhibiting inflammatory reaction.
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Objective:To investigate the effect of extract of gingko biloba (EGB)on inflammatory cytokines in patients with early diabeti cnephropathy(DN). Methods:Thirty-nine patients with early DN were randomly assigned into the control group (n=19)treated by irbesartan alone and the treated group (n=20)treated by EGB combined with irbesartan for 6 months. Serum interleukin-6(IL-6)and tumor necrosis factor-?(TNF-?)were determined with ELISA before and after treatment,and urinary albumin excretion rate,fasting blood glucose,serum creatinine,blood urea nitrogen and lipid profiles,were examined as well. Results:The levels of IL-6 and TNF-? were significantly lower in both groups after treatment than those before treatment (P
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OBJECTIVE:To investigate the expression of matrix metallopro teinase-2(MMP-2),tissue inhibitor of metalloproteinase-2(TIMP-2) and collagen Ⅳ(Ⅳ-C) in kidney of diabetic rats and the intervention effect of irbesartan.METHODS:30 male SD rats were randomly divided into three groups:normal control group(NC group),diabetes mellitus group(DM group) and diabetes mellitus+50 mg?kg-1 irbesartan group(DI group).DM and DI group were injected with streptozocin intraperitoneally to reduce diabetic model.After 8 weeks the expression of MMP-2,TIMP-2 and Ⅳ-C in kidney tissure were determined by immunohistochemistry method(SP) and RT-PCR.RESULTS:Compared with NC group,DM group showed the expression of protein and mRNA of MMP-2 significantly reduced while that of TIMP-2 and Ⅳ-C increased(P