RESUMO
Once pulp necrosis or apical periodontitis occurs on immature teeth, the weak root and open root apex are challenging to clinicians. Berberine (BBR) is a potential medicine for bone disorders, therefore, we proposed to apply BBR in root canals to enhance root repair in immature teeth. An in vivo model of immature teeth with apical periodontitis was established in rats, and root canals were filled with BBR, calcium hydroxide or sterilized saline for 3 weeks. The shape of the roots was analyzed by micro-computed tomography and histological staining. In vitro, BBR was introduced into stem cells from apical papilla (SCAPs). Osteogenic differentiation of stem cells from apical papilla was investigated by alkaline phosphatase activity, mineralization ability, and gene expression of osteogenic makers. The signaling pathway, which regulated the osteogenesis of SCAPs was evaluated by quantitative real time PCR, Western blot analysis, and immunofluorescence. In rats treated with BBR, more tissue was formed, with longer roots, thicker root walls, and smaller apex diameters. In addition, we found that BBR promoted SCAPs osteogenesis in a time-dependent and concentration-dependent manner. BBR induced the expression of β-catenin and enhanced β-catenin entering into the nucleus, to up-regulate more runt-related nuclear factor 2 downstream. BBR enhanced root repair in immature teeth with apical periodontitis by activating the canonical Wnt/β-catenin pathway in SCAPs.
Assuntos
Animais , Masculino , Ratos , Berberina , Farmacologia , Diferenciação Celular , Papila Dentária , Osteogênese , Periodontite Periapical , Terapêutica , Células-Tronco , Biologia Celular , Metabolismo , Via de Sinalização Wnt , Proteína Wnt3A , Genética , Metabolismo , Microtomografia por Raio-XRESUMO
Objective To analyze and identify the chemical constituents of essential oil from the fruits of Cinnamomum camphora chvar. Borneol. Methods The essential oil from the fruits was extracted by steam distillation and its chemical constituents were analyzed and identified by GC-MS. Results Fifty compounds were separated, and 42 kinds of which accounting for 99.536% were identified. D-borneol was the most abundant compound, of which the amount was 50.684%of the total constituents. Conciusion This present study demonstrated higher content of natural D-borneol, providing scientific basis for further exploration and utilization of the fruits of Cinnamomum camphora chvar. Borneol.