HPLC-ELSD in determination of AR-I content in total furostanol saponins of Asparagus cochinchinensis / 第二军医大学学报

Objective: To determine AR-I content in total furostanol saponins of Asparagus cochinchinensis. Methods: High performance liquid chromatogra phy (HPLC) was used and the condition was: Diamonsil C18 column (5 mm×250 mm, 5 μm); mobile phase: CH3CN-H2O (36 : 64); flow rate: 0.8 ml/min; injector volume:20 μl;colum temperature: room temperature. Evaporative light scattering detector(ELSD) condition was: drift tube temperature: 40°C; and nebulizer gas pressure (N2): 2.0×105 Pa. Results: The linearity was good when AR-I was within the range of 0.094-0.940 0 g · L-1 (r=0.999 2). The test had higher sensitivity and good stability. The average recovery was 101.3% (n=6)and RSD was 2.36%. Conclusion: HPLC-ELSD is practical and reliable for the determination of the furostanol saponins of Asparagus cochinchinensis.

Studies on chemical constituents of Ervatamia hainanensis / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Ervatamia hainanensis.</p><p><b>METHOD</b>The compounds were separated and purified by column chromatography with silica gel, and identified by IR, MS, NMR and 2D-NMR.</p><p><b>RESULT</b>Five compounds were identified as I (isolariciresinol 9-O-beta-D-glucopyranoside), II (cycloartenol), III (beta-amyrin acetate), IV (beta-sitosterol), V (daucosterol), respectively.</p><p><b>CONCLUSION</b>All the compounds were isolated from this plant for the first time.</p>

Influence of total saponins from Asparagus cochinchinensis on cerebral blood flow and vascular resistance in anesthetized dogs / 第二军医大学学报

Objective:To study the effect of total saponins on cerebral blood flow and vascular resistance in anesthetized dogs.Methods:Thirty hybrid dogs in either sex,with a body weight of(11?1.5)kg,were evenly randomized into 5 groups: negative control group(saline 5 ml/kg,ig),positive control(nimodipine 300?g/kg,iv),and 3 groups treated with total saponins (low-dose group[10 mg/kg,ig],middle-dose group[30 mg/kg,ig],and high-dose group[60 mg/kg,ig]).The dogs were anes- thetized with intravenous pentobarbital sodium(30 mg/kg).The right common carotid artery was exposed to measure the cere- bral blood flow,cerebral vascular resistance,blood pressure and heart rate using the MFV-3200 electromagnetic flow meter and MPA-3000 bioelectricity signal-amplifier.Results:Compared with negative control,cerebral blood flow was significantly in- creased in animals treated with asparagus root saponins(30 and 60 mg/kg,ig)during 5 and 120 min after drug administration (P