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Objective This study was to evaluate the safety of 640 corneal donors by analysing the serological testing results.Methods We retrospectively analyzed the serological testing results from Changsha Aier Eye Bank and Chengdu Kangqiao Aier Eye Bank from January 2011 to December 2015,hepatitis B virus surface antigen (HBsAg),hepatitis C virus (HCV),treponema pallidum (TP) and human immunodeficiency virus (HIV) were detected by colloidal gold or enzyme-linked immunosorbent assay (ELISA).Results There were 83 out of 640 serum samples showed positive immuno-reaction assayed markers,the positive rate was 12.97％,including HBsAg(n=60,9.38％),HCV(n=3,0.47％),TP(n=11,1.72％) and HIV(n=2,0.31％).Moreover,3 corneal donors were both positive against HBsAg and HCV,2 donors positive against HCV and TP,1 donor positive against HBsAg and HIV,1 donor positive against HBsAg and TP.Conclusions There is a high proportion of positive results of blood-borne diseases in cornea donors,which is a potential threat to corneal receptors and eye bank workers.Therefore,it is very important to detect serological test strictly for corneal donors.
RESUMO
Objective To compare the precision and correlation of test parameters betwee the two types of automatic blood ana-lyzer,Sysmex XT-2000i and Mindray BC-5800.Methods We selected 60 cases of EDTA-K2 anticoagulant whole blood samples, correlation analysis was carried out on the two instrument parameters,and high,medium and low value samples were chosen for 10 times′precision analysis in the two instrument s respectively.Results The variation coefficient(CV)values of blood count parame-ters of Sysmex XT-2000i were 0.02%-5.70%,while the CV values of white blood cell classification parameters were 0.06%-1. 66%.The CV values of blood count parameters of Mindray BC-5800 were 0.02%-5.3%,white the CV values of white blood cell classification parameters were 0.09%-1.17%.The determination coefficient(r2 )of each parameter of the two instruments were 0. 185-0.995.Conclusion the correlation between the two instruments is good,and the two instruments′precisions were consistent with the design range.