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Objective:To investigate the effect of electroacupuncture on hemiplegia after stroke.Methods:A total of 102 patients with post-stroke hemiplegia who received treatment in Zhejiang Medical and Health Group from November 2018 to November 2019 were included in this study. They were randomly assigned to receive routine rehabilitation training (control group, n = 51) or routine rehabilitation training combined with electroacupuncture (study group, n = 51) for 2 weeks. Before and after 2 weeks of treatment, traditional Chinese medicine symptom scores (facial paralysis, sensory disturbance, limb numbness) and gait time-space parameters (10-m maximum walking speed, stride length, strides frequency), limb function (modified Barthel index, Fugl-Meyer Assessment score) and quality of life (Stroke Specific Quality of Life Scale) were compared between the control and study groups. Results:After treatment, facial paralysis score [(2.15 ± 0.63) points vs. (1.12 ± 0.71) points, (2.14 ± 0.60) points vs. (1.65 ± 0.74) points], sensory disturbance score [(2.45 ± 0.23) points vs. (1.32 ± 0.73) points, (2.46 ± 0.25) points vs. (1.88 ± 0.63) points] and limb numbness score [(1.89 ± 0.73) points vs. (1.04 ± 0.53) points, (1.88 ± 0.67) points vs. (1.42 ± 0.48) points] in the study and control groups were significantly decreased compared with before treatment. These scores after treatment in the study group were significantly lower than those in the control group ( t = 3.691, 4.147, 3.795, all P < 0.001). After treatment, 10-m maximum walking speed [(0.34 ± 0.15) m/s vs. (0.53 ± 0.21) m/s, (0.33 ± 0.15) m/s vs. (0.42 ± 0.16) m/s], stride length [(61.54 ± 16.04) cm vs. (78.93 ± 20.48) cm, (59.80 ± 17.35) cm vs. (69.68 ± 18.32) cm], stride frequency [(65.52 ± 10.98) step/min vs. (78.12 ± 12.04) step/min, (63.10 ± 10.89) step/min vs. (71.15 ± 9.28) step/min] in the study and control groups were significantly increased compared with before treatment. These outcomes after treatment in the study group were superior to those in the control group ( t = 2.976, 2.404, 3.274, all P = 0.001). After treatment, modified Barthel index [(44.94 ± 3.29) vs. (75.27 ± 3.56), (43.32 ± 4.51) vs. (64.51 ± 3.95)], Fugl-Meyer Assessment score [(52.36 ± 6.89) points vs. (73.20 ± 5.24) points, (52.35 ± 6.79) points vs. (61.97 ± 4.88) points] and Stroke Specific Quality of Life Scale score [(9.42 ± 1.95) points vs. (15.69 ± 1.78) points, (9.24 ± 2.32) points vs. (13.65 ± 1.96) points; (8.97 ± 2.58) points vs. (19.52 ± 1.35) points; (9.48 ± 1.77) points vs. (16.20 ± 1.72) points; (12.32 ± 2.56) points vs. (25.26 ± 2.45) points; (12.41 ± 2.50) points vs. (18.20 ± 2.44) points; (13.03 ± 3.16) points vs. (20.07 ± 2.67) points; (12.49 ± 2.77) points vs. (16.60 ± 2.78) points; (5.98 ± 1.23) points vs. (12.30 ± 1.36) points; (5.99 ± 1.21) points vs. (8.25 ± 1.37) points] in the study and control groups were significantly increased compared with before treatment. After treatment, these scores in the study group were significantly higher than those in the control group ( t = 14.451, 11.200, 5.502, 10.843, 14.581, 6.429, 14.983, all P < 0.001). Conclusion:Electroacupuncture can effectively improve the symptoms and limb function of patients with post-stroke hemiplegia, and improve their gait and quality of life.
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Objective@#To establish a method for determination of methyl ethyl ketone in urine by headspace gas chromatography.@*Methods@#In the urine sample(hereinafter referred to as urine sample), methyl ethyl ketone is pretreated by headspace technology, and a certain amount of head air is injected into the gas chromatograph, separated by capillary column, detected by hydrogen flame ionization detector, and the retention time is qualitative and the peak height is high. Peak area.@*Results@#Good linearity was in the range of 0.01 to 6.0 μg/ml with a regression equation of y=13.316x+0.8497 and γ=0.9997.The minimum detectable concentration of methyl ethyl ketone was 0.01 μg/ml. The range intra-day RSD and inter-day RSD were 2.2%-5.5% and 2.5%-6.1% respectively. Urine samples can be stored for 20 days in the refrigerator at 4 ℃.@*Conclusion@#The method has a high advantage of sensitivity and accuracy, and also easy to operate. Therefore, it is suitable for the determination of methyl ethyl ketone in urine.
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Curcumin is derived from traditional Chinese medicine turmeric and can be used for chemoprevention and treatment of various cancers. Recent studies have shown that curcumin can participate in the regulation of various molecular signal transduction pathways, regulate the levels of reactive oxygen species, and regulate the inflammatory microenvironment to inhibit the occurrence of tumors. In addition, curcumin can inhibite the proliferation of tumor cells and promote apoptosis of tumor cells. Here, the paper reviews the mechanism of action of curcumin in cancer prevention and treatment and the new progress in the clinical trials, aiming to provide a basis for research in related fields.
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Curcumin is a polyphenol pigment product from the rhizome of curcumin longa.It is associated with numerous therapeutic benefits,including antioxidant,anti-inflammatory and anticancer activities.Research shows that EF24 is a curcumin analog that can induce tumor cell apoptosis,arrest cell cycle,inhibit cell invasion and metastasis and has synergistic effect of combined antitumor agents.EF24 has improved anticancer activity and bioavailability against various cancer cell lines over curcumin without increased toxicity.EF24 would have great potential to be used as a useful therapeutic agent for the treatment of various types of cancer.
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<p><b>OBJECTIVE</b>To explore the effects of electroacupuncture (EA) on behavioral function and synaptic plasticity in hippocampal CA3 area in rats with chronic stress depression.</p><p><b>METHODS</b>According to the random number table method, 144 SD male rats were assigned into a blank group, a model group, an EA group and a fluoxetine group, then each group was divided into a 7 d subgroup, a 14 d subgroup and a 21 d subgroup, 12 rats in each subgroup. The chronic mild unpredictable stress stimulus combined with lonely breeding were applied to establish the depression model of rats, which was performed simultaneously with intervention treatment. The rats in the EA group were treated with EA (dilatational wave) at "Shenting" (GV 24) and "Baihui" (GV 20), while the rats in fluoxetine group were treated with intragastric administration of fluoxetine, once daily. With open-field test, sugar consumption experiment and transmission electron microscope, the changes of behavior and neuronal synapse inhippocampal CA3 area were observed.</p><p><b>RESULTS</b>On 7 d, 14 d and 21 d, compared with the blank group, the open-field test score, sugar consumption and body mass were significantly lower in the model group (all<0.01); compared with the model group, the open-field test score, sugar consumption and body mass were significantly higher in the EA group and the fluoxetine group on 14 d and 21 d (<0.01,<0.05). On 14 d and 21 d, compared with the blank group, the synapse in hippocampal CA3 area was significantly lower in the model group (both<0.01); compared with the model group, the synapse in hippocampal CA3 area was significantly higher in the EA group and the fluoxetine group (<0.01,<0.05). The neurons cells in hippocampal CA3 area in the model group showed pyknosis and deformation from 7 d with fusion structure and unclear boundary of synapse, which were significantly improved on 21 d; the neurons cells in hippocampal CA3 area in the EA group and the fluoxetine group were significantly improved from 14 d and restored to normal level on 21 d, in addition, the structure of synapse restored to normal level.</p><p><b>CONCLUSIONS</b>EA is involved in the regulation of synaptic plasticity in hippocampal CA3 area, and promotes the recovery of depression symptoms.</p>
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To explore the best measurement of waist circumference related with intra-abdominal fat area evaluated by magnetic resonance imaging ( MRI). Totally 207 participants aged 20-60 years were enrolled. Waist circumference were measured at the levels of navel ( WC1) and the midpoint between costal brim and iliac cest (WC2). Intra-abdominal fat area was evaluated by MRI scan. Intra-abdominal fat area was significantly higher in men than in women [(132. 17 ± 59. 49 vs 70. 56 ± 35. 33)cm2 , P<0. 01]. Pearson correlation analysis showed that WC1 and WC2 were positively correlated with intra-abdominal fat area (r = 0. 779, r = 0. 809, both P<0. 01). Multiple linear stepwise regression analysis demonstrated that WC1 and WC2 were independently associated with intra-abdominal fat area(β=0. 553, R2 =0. 714, P<0. 01; β = 0. 603, R2 = 0. 735, P<0. 01). All of the two different measurements of waist circumference parameters may reflect intra-abdominal fat area, while WC1 seems to be the simpler one.
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Objective To observe the changes of serum and fecal taurine-conjugated bile acid levels and its association with glucose metabolism during the spontaneous development of type 2 diabetes in OLETO rats.Methods Twenty male OLETF rats(4 weeks old)were included and 10 male LETO rats of the same age were used as the normal control group.OLETF rats were fed with high fat diet whereas LETO rats were fed with normal diet.Serum and fecal taurine-conjugated bile acid levels of OLETF rats were tested at different stage of diabetes including baseline, normal glucose tolerance, impaired glucose tolerance and diabetes periods, and the association of taurine-conjugated bile acid level with body weight, blood glucose, and glucose-regulating hormones were also investigated.Results Compared with LETO rats, the baseline serum levels of taurine-conjugated bile acid in OLETF rats did not change, but the levels of fecal taurine-conjugated bile acid including taurine-conjugated chenodeoxycholic acid(TCDCA), taurocholic acid(TCA)and taurine-conjugated deoxycholic acid(TDCA)were significantly decreased [(14.25±7.18 vs 0.90±0.31)mg/kg,(7.12±4.14 vs 1.30±0.35)mg/kg,(4.30±1.78 vs 1.02±0.14)mg/kg, all P<0.01].During the development of diabetes, the fecal levels of TCDCA, TCA and TDCA were still lower than those in the control rats.TDCA was negatively associated with the level of fasting blood glucose(r=-0.470, P=0.032),but positively associated with the serum level of glucagon-like peptide(GLP)-l(r=0.406, P=0.044).Conclusion The decrease of intestinal taurine-conjugated bile acid level is involved in the development of diabetes in OLETF rats.Intestinal TDCA may regulate the secretion of GLP-1 by paracrine pathway.
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To explore the best measurement of waist circumference related with intra-abdominal fat area evaluated by magnetic resonance imaging ( MRI). Totally 207 participants aged 20-60 years were enrolled. Waist circumference were measured at the levels of navel ( WC1) and the midpoint between costal brim and iliac cest (WC2). Intra-abdominal fat area was evaluated by MRI scan. Intra-abdominal fat area was significantly higher in men than in women [(132. 17 ± 59. 49 vs 70. 56 ± 35. 33)cm2 , P<0. 01]. Pearson correlation analysis showed that WC1 and WC2 were positively correlated with intra-abdominal fat area (r = 0. 779, r = 0. 809, both P<0. 01). Multiple linear stepwise regression analysis demonstrated that WC1 and WC2 were independently associated with intra-abdominal fat area(β=0. 553, R2 =0. 714, P<0. 01; β = 0. 603, R2 = 0. 735, P<0. 01). All of the two different measurements of waist circumference parameters may reflect intra-abdominal fat area, while WC1 seems to be the simpler one.
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<p><b>OBJECTIVE</b>To investigate the effect of occupational exposure to toluene diisocyanate (TDI) on the workers' health.</p><p><b>METHODS</b>A total of 76 workers exposed to TDI (exposure group) and 64 management staff members (control group) were selected from a factory as the study subjects. Area sampling was performed for the place with exposure to TDI according to the method in GBZ 159-2004 Specifications of air sampling for hazardous substances monitoring in the workplace, and gas chromatography was applied to measure the concentration of TDI in workplace air. The workers' personal information was collected with questionnaire, pulmonary ventilation function was determined with a portable spirometer, hematological parameters were analyzed by automatic blood analyzer and blood chemistry analyzer, and the indicators of oxidative damage and energy metabolism were measured by the reagent kit provided by Nanjing Jiancheng Bioengineering Institute. SPSS 17 software was applied for statistical analysis.</p><p><b>RESULTS</b>The exposure group had significantly lower forced vital capacity (FVC), forced expiratory volume in 1 second(FEV1.0), and FEV1.0/FVC ratio than the control group (P <0.05). Compared with the control group, the exposure group had significantly higher red blood cell count, platelet distribution width, mean platelet volume, lymphocyte count, and neutrophil count(P<0.01), and significantly lower activities of lactate dehydrogenase(LDH), superoxide dismutase, and succinodehydrogenase (SDH)(P <0.01). In the exposure group, the length of exposure was negatively correlated with the activities of SDH and LDH in the serum (r=-0.319, P <0.05; r=-0.239, P <0.05), and the length of exposure was not found to be correlated with the activity of SOD and pulmonary function indices.</p><p><b>CONCLUSION</b>TDI can induce inflammatory response and lung ventilation function impairment in workers exposed to TDI, as well as oxidative stress and imbalance of energy metabolism. Therefore, it can cause damage to workers' health, and protective measures should be enhanced.</p>
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Humanos , Estudos de Casos e Controles , Contagem de Eritrócitos , Volume Expiratório Forçado , Inflamação , L-Lactato Desidrogenase , Sangue , Metabolismo , Contagem de Leucócitos , Pulmão , Exposição Ocupacional , Ventilação Pulmonar , Succinato Desidrogenase , Sangue , Metabolismo , Superóxido Dismutase , Metabolismo , Tolueno 2,4-Di-Isocianato , Capacidade VitalRESUMO
Objective The aim of the study was to explore the relationship between hypertriglyceridemic-waist(HTWC) phenotype and clustering of cardiovascular risk factors in adolescents. Methods A cross-sectional study was conducted in 3 136 Han adolescents aged 13-17 years in Qinhuangdao. The HTWC phenotype was defined as serum triglyceride concentrations ≥1. 47 mmol/ L and waist circumference ≥90 th percentile for age and sex. Results The prevalence of HTWC phenotype was 3. 3% . The prevalence of clustering of cardiovascular risk factors was 0. 4%in adolescents with normal waist circumference and serum triglyceride and 14. 7% in adolescents with HTWC phenotype. After adjustment for sex, age, body mass index, and family history of diabetes and hypertension, the prevalence of clustering of cardiovascular risk factors in adolescents with HTWC phenotype was 9. 286 fold(95% CI 3. 161-27. 282, P<0. 01) of that in adolescents with normal waist circumference and triglyceride. Conclusion There was a significant correlation between HTWC phenotype and clustering of cardiovascular risk factors, suggesting that this phenotype may be applied as a simple marker for identifying adolescents with clustering of cardiovascular risk factors.
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A mixture of two new anomers: 3,4-di-O-[syringate]-alpha-D-glucopyranose (1) and 3,4-di-O-[syringate]-beta-D-glucopyranose (2), together with syringic acid (3), were isolated from the alcohol extract of Tagetes erecta. The structures of these compounds were elucidated by various spectroscopic methods, including intensive 1D, 2D NMR and HR-ESI-MS studies. The structural assignment was further supported by HPLC-ESI-MS with compound 1 at t(R) = 3.22 min (m/z 538.9 [M-H](-)) and compound 2 at 3.25 min (m/z 538.9 [M-H](-)) in the mixture.
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Flores , Química , Ressonância Magnética Nuclear Biomolecular , Extratos Vegetais , Química , Tagetes , Química , Taninos , QuímicaRESUMO
An unusual natural product named 2,4,6-trihydroxacetophenone-3, 5-di-C-beta-D-glucoside (18) were isolated from aerial parts of Rhododendron lepidotum grows in Tibet, along with other 17 known compounds: hopenol-B (1), lupeol (2), ursolic acid (3), avicularin (4), quercetin (5), myricetin (6), hyperoside (7), myricetin-3'-O-beta-D-xyloside (8), (+)-taxifolin-3-O-alpha-L-arabinopyranoside (9), (+)-taxifolin-3-O-beta-D-glucopyranoside (10), lyoniside (11), confluentin (12), 2-(4-hydroxyphenyl)-ethyltriacontanoate (13), 2, 6-dimethoxy-4-hydroxyphenyl-1-O-beta-D-glucopyranoside (14), (-)-2-hydroxy-5-(2-hydroxyethyl) phenyl-O-beta-D-glucopyranoside (15), (-)-isola-riciresinol (16) and isofraxoside (17). All the compounds were firstly isolated from this plant as well as compounds 1, 13-18 were reported occurrence in R. spp. for the first time. The structures were identified based on analyses of spectroscopic data and physico-chemical evidences.
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Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas , Química , Compostos Orgânicos , Rhododendron , QuímicaRESUMO
ObjectiveTo study the constituents in the twigs and leaves of Rhododendron primulaeflorum.MethodsThe constituents were separated and purified with chromatographic methods.Their structures were elucidated by spectroscopic methods (1D,2D NMR,IR,and HR-ESI-MS) and chemical analyses.Results One new phenylpropanol glycoside,4-hydroxyl-5-methoxyl-phenylpropanol-3-O-β-D-glucopyranoside (1),and its aglycone (2) were successfully isolated from the twigs and leaves of R.primulaeflorum.ConclusionCompound 1 is a new phenylpropanol glycoside.Compounds 1 and 2 are isolated from this plant for the first time.