Estudo da técnica de denervação da cápsula articular coxofemoral no tratamento da dor em cães com displasia coxofemoral: resultados preliminares

O artigo não apresenta resumo.

Ca2+ dependence of gluconeogenesis stimulation by glucagon at different cytosolic NAD+-NADH redox potentials

The influence of Ca2+ on hepatic gluconeogenesis was measured in the isolated perfused rat liver at different cytosolic NAD+-NADH potentials. Lactate and pyruvate were the gluconeogenic substrates and the cytosolic NAD+-NADH potentials were changed by varying the lactate to pyruvate rations from 0.01 to 100. The following results were obtained: a) gluconeogenesis from lactate plus pyruvate was not affected by Ca2+-free perfusion (no Ca2+ in the perfusion fluid combined with previous depletion of the intracellular pools); gluconeogenesis was also poorly dependent on the lactate to pyruvate rations in the range of 0.1 to 100; only for a ratio equal to 0.01 was a significantly smaller gluconeogenic activity observed in comparison to the other rations. b) In the presence of Ca2+, the increase in oxygen uptake caused by the infusion of lactate plus pyruvate at a ratio equal to 10 was the most pronounced one; in Ca2+-free perfusion the increase in oxygen uptake caused by lactate plus pyruvate infusion tended to be higher for all lactate to pyruvate ratios; the most pronounced difference was observed for a lactate/pyruvate ratio equal to 1.c) In the presence of Ca2+ the effects of glucagon on gluconeogenesis showed a positive correlation with the lactate to pyruvate rations; for a ratio equal to 0.01 no stimulation ocurred, but in the 0.1 to 100 range stimulation increased progressively, producing a clear parabolic dependence between the effects of glucagon and the lactate to pyruvate ratio. d) In the absence of Ca2+ the relationship between the changes caused by glucagon in gluconeogenesis and the lactate to pyruvate ratio was substantially changed; the dependence curve was no longer parabolic but sigmoidal in shape with a plateau beginning at a lactate/pyruvate ratio equal to 1; there was inhibition at the lactate to pyruvate ratios of 0.01 and 0.1 and a constant stimulation starting with a ratio equal to 1; for the lactate to pyruvate ratios of 10 and 100, stimulation caused by glucagon was much smaller than that found when Ca2+ was present. e) The effects of glucagon on oxygen uptake in the presence of Ca2+ showed a parabolic relationship with the lactate to pyruvate ratios which was closely similar to that found in the case of gluconeogenesis.

Alteracoes circulatorias na microcirurgia da laringe. / Circulatory changes in the microsurgery of the larynx

Foi realizado um estudo em 20 pacientes adultos, classe I e II ASA, submetidos a microcirurgia da laringe, visando avaliar as alteracoes circulatorias nesse tipo de procedimento cirurgico que determina manuseio prolongado de estrutura altamente reflexogena. Observou-se elevacao da pressao arterial (PA) e da frequencia cardiaca (FC) durante as manobras de intubacao traqueal realizadas pelo anestesista, que diminuiram gradualmente durante a microcirurgia, com a ressalva de que a FC nao decresceu uniformemente. Alteracoes do ECG foram registradas em 75% dos pacientes e apareceram com maior frequencia durante a microcirurgia. Algumas disritmias pela sua gravidade poderiam comprometer a vida do paciente e, para minimizar este problema, e recomendado que se utilize para este tipo de procedimento cirurgico anestesia topica da laringe ou plano profundo de anestesia