Rare chromosome structural aberration characterizing oncology malignancy

Ring chromosome aberration are rare abnormality potentially involving any chromosome in patients diagnosing in Oncology. The present review and case study has focused on the ring chromosome associated with oncology malignancies. An electronic peer review article search was performed systematically to obtain relevant literature with the CINAHL, Google scholar, and Pub Med databases. The keywords included marker, abnormalities, structural, Ring chromosome. The inclusion criteria for the review were that the documents were original quantitative research and published in English. This was also initiated using Medline, Mitelman database [http:/cgap.nci.nih.gov/Chromosomes/Mitelman], Danish cytogenetic register and other pertinent web references on ring chromosomes in Oncology malignancies. Articles that were not directly relevant to the present objective were excluded. Also the un-stimulated bone marrow specimen of present case manipulated with Methotrexate cells culture synchronization and finally was treated by GTG-banding technique. Ring chromosome was observed in 10% of the total cells. Cytogenetic analysis demonstrated apparently ring [15] 46, XY, r[15] karyotype. The clinical findings revealed history of nausea, loss of appetite, diarrhea, night sweats, and a weight loss, anemia and diagnosed as accelerated CML. Our finding adds to the spectrum of both morphology and genetic rearrangements in oncology malignancies. Additional future analyses in similar subject will be necessary to draw firm conclusions

Performance of a biotrickling filter employing Thiobacillus thioparus immobilized on polyurethane foam for hydrogen sulfide removal

The removal of hydrogen sulfide [H[2]S] from contaminated airstream was studied in a biotrickling filter [BTF] packed with open-pore polyurethane foam as a carrier of Thiobacillus thioparus [DSMZ5368] with counter current gas/liquid flows. The effect of operating parameters on BTF performance was studied. Experiments were performed at different Empty Bed Residence Times [EBRT] from 9 to 45 seconds, and different initial H[2]S concentration from 25 to 85 ppm. The results showed reasonable performance of the BTF, in H[2]S removal from the synthetic gas stream. However, the performance was somewhat lower than other studies in BTF in which either Thiobacillus thioparus with other packings or polyurethane foam with other microbial cultures were used. The effect of liquid recirculation rate [LRR] in the range of 175-525 ml/min [0.46-1.34 m/h] on BTF performance was also studied. Results showed that increasing LRR from 175 to 350 mL/min resulted in significant enhancement of H2S removal efficiency, but further increase in LRR up to 525 mL/min had an insignificant effect. H[2]S elimination at different heights of the bed was studied and it was found that decrease in EBRT results in more homogeneous removal of the pollutant in BTF. Determination of microbial species in the BTF after 100 days performance showed that during BTF operation the only H[2]S degrading specie was Thiobacillus thioparus

[Conjugation of anti-morphine antibody to gold nanoparticles synthesized with glutamic acid]

Gold nanoparticles are one of the most applicable metal nanoparticles which are widely being used in different fields such as nanomedicine and nanobiotechnology. The goal of this study is the production of gold nanoparticles with appropriate size by the use of Glutamic acid which is considered as a green method. For diagnostic application the synthesized gold nanoparticles would be than conjugated with anti-morphine antibody. In this experimental study, gold nanoparticles were synthesized and coated by chemical reduction method with the use of Glutamic acid. Formation of gold nanoparticles and their size were recognized and measured by UV-Vis spectroscopy, Dynamic light scattering and Transmission electron microscopy. Anti-morphine antibody was purified and prepared by subcutaneous injection of 400 micro g/ml dose of morphine vaccine to 9 rabbits. Study of immunity, immunogenicity and antibody production was performed by immuno-diffussion methods. After production and recognition of colloidal gold nanoparticles they were changed into suspension by the use of PBS buffer. They were then conjugated with anti-morphine antibody and incubated at 4 degree C for 115 hours. Conjugated nanoparticles were characterization by SDS-PAGE and UV-V is spectroscopy. The highest absorption of these particles was at 525 nm and their size was about 20 nm formed bands pertaining to conjugated nanoparticles were displaced in compare with standard samples. Glutamic acid is capable of synthesizing gold nanoparticles and since amino acids are intoxic, the nanoparticles which are synthesized by the use of amino acids, would be applicable in medicine and biotechnology. Nanoparticles conjugated with antibodies, could be also used as recognition tools.

Surveying Desferal production increment by inducing mutation in Streptomyces griseoflavus

Desferal or Desferrioxamine B mesylate is an iron chealator drug. This medicine decreases the iron overload in the thalassemia patients who have been blood transfused; the excess of iron is excreted through bile or urine. Novartis is the sole company which produces desferrioxamine B mesylate in the world and our country is importer of such drug. Thus we tried to increase Desferal production by inducing mutation in Streptomyces griseoflavus. This is an applied research carried out at pilot level. The organism is a Gram-positive bacterium that was supplied in lyophilized by Persian Type culture Collection, Iranian Research Organization for Science and Technology [IROST], Tehran, Iran bearing the code no. PTCC1130, which was cultured on Des4 medium. The organism was mutated by UV irradiation hence selective techniques and markers were employed to distinguish marked strains from parent S. griseoflavus. When the mutated organisms were selected according to their characteristics and used to fuse their protoplasts in order to obtain high yield desferrioxamine producing recombinant Streptomyces griseoflavus. The varied parameters were bacterial growth rate and desferal concentration in the culture broth. Our study showed that the rate of desferal production in mutant's strains called C7031 and S7011 and fusants srains called FP10 and FP9 was higher than wiled type Streptomyces griseoflavus. The increment in production of desferrioxamine was found to be 68% in FP9 and 81% in FP10 fusants. The mutation and protoplasts fusion of Streptomyces griseoflavus caused increment in production of desferrioxamine. The infrared spectrum, thin layer chromatogram of desferrioxamine extracted from culture broth was similar to that of standard desferrioxamine [Novartis] from the point of molecular identity

Safety and immunogenecity of human therapeutic morphine vaccine in morphine addicts

Morphine vaccine is a product of morphine-6- succinate synthesis and its conjugation with albumin serum. Each dose contains 0.5 mg Aluminum hydroxide, 8 mg sodium chloride, 1.12 mg di- sodium hydrogen phosphate, 1.1 mg sodium di- hydrogen phosphate and 50 mg morphine-6-succinate albumin serum. Humoral safety is achieved in morphine addicts following three successive doses within 0-30-60-day intervals. Its immunogenecity brings about withdrawal without the risk of abstinence syndrome. This study was conducted to examine the effect morphine vaccine on morphine addicts. Based on the Ethics protocol of Pasteur Institute of Iran, this clinical trial was conducted on 102 male volunteer addicts [mean age 25 years]. Variables included vaccine dosage and concentration on of antibodies. Volunteers were divided into groups of 30 [experimental] and 4 [placebo]. Adjuant was injected to placebo group addicts; the three experimental groups were given 5, 12, 100 and 600 microg within 0-30-60 days interval through injection to deltoid; all subjects referred for follow-up on the 90[th] day. Blood samples and antibody evaluation was obtained from all three groups in months 5, 7, 9, 11 and 12. A positive correlation was observed between antibody production and vaccine dosage as well as frequency of injection. Anti-morphine antibody was detected after the first injection of 100 microg/ml, 600 microg/ml and second injection of 12.5 microg/ml doses. Antibody levels reached the peak by three months and did not decline to the baseline after one year. The vaccine was well tolerated and lacked the reverse and unfavorable effects, characteristic of vaccines and drugs. On day 90, humoral immunity was created in all patients