RESUMO
A method for qualitative analysis of constituents in Panax notoginseng by UPLC-LTQ-Orbitrap mass spectrometry was established. Based on the high-resolution mass information, MS/MS fragmentation behaviors and chemical components from literatures, 43 compounds were identified or tentatively characterized. New type saponin aglycone, combined with malonyl-substituted and acetyl-substituted saponins were discovered and plausibly identified in this study. This work could be helpful for the quality control and further phytochemical studies of Panax notoginseng, and provided a good example for the analysis of chemical constituents in traditional Chinese medicine.
Assuntos
Cromatografia Líquida de Alta Pressão , Análise de Fourier , Ginsenosídeos , Panax notoginseng , Química , Raízes de Plantas , Química , Plantas Medicinais , Química , Controle de Qualidade , Saponinas , Classificação , Espectrometria de Massas em TandemRESUMO
Five compounds, huyouyisu (1), vomifoliol (2), isoferulic acid (3), 1,2,3-trihydroxyphenol (4) and p-hydroxy-phenol (5), were isolated from the peels of Citrus changshan-huyou Y. B. Chang for the first time by utilizing repeated column chromatography on silica gel. Among them, huyouyisu (1) is a new compound. The structure was elucidated by using 1D and 2D spectra.
Assuntos
Butanóis , Química , Cinamatos , Química , Citrus , Química , Cicloexanonas , Química , Estrutura Molecular , Fenóis , Química , Plantas Medicinais , QuímicaRESUMO
<p><b>OBJECTIVE</b>To study the effects of soil microbes of habitats, endophytic fungi and root surface fungi of Huperzia serrata on rooting rate of the cuttings and on several physiological and biochemical indexes.</p><p><b>METHOD</b>The cuttings of H. serrata were planted on soil I of primary habitat, the soil II had been inoculated endophytic fungi and root surface fungi, the soil III had been auto-claved, rooting rate was examined, and soluble protein and soluble sugar contents, activities of PPO and POD, flavonoids and huperzine A contents were determined.</p><p><b>RESULT</b>Compared with the H. serrata that were planted on soil III, soil I and soil II were found to increase the rooting rate by 10% and 16%, soil II increased the soluble protein contents of stem of H. serrata (P < 0.05), Soil I increased the soluble sugar contents of leaves and stem of H. serrata (P < 0.05), soil I and Soil II increased the flavonoids contents of H. serrata (P < 0.05).</p><p><b>CONCLUSION</b>Soil microbes from primary habitat, endophytic fungi and root surface fungi promote rooting, they also increase plant metabolism level of H. serrata.</p>
Assuntos
Metabolismo dos Carboidratos , Flavonoides , Metabolismo , Huperzia , Metabolismo , Microbiologia , Proteínas de Plantas , Química , Metabolismo , Raízes de Plantas , Metabolismo , Microbiologia , Microbiologia do Solo , SolubilidadeRESUMO
<p><b>OBJECTIVE</b>To study the chemical constituents of Eriophyton wallichii.</p><p><b>METHOD</b>Compounds were separated and purified by column chromatographic methods, and their structures were elucidated by spectroscopic methods.</p><p><b>RESULT</b>Eight phenylpropanoids were isolated and identified as martynoside (1), leucosceptoside A (2), citrusin B (3), (+)-dehydrodiconiferyl alcohol-4, 9-beta-D-glucopyranoside (4), liriodendrin (5), velutinoside 11[ (6), jionoside B, (7), stachysoside D (8), respectively.</p><p><b>CONCLUSION</b>The eight compounds were firstly isolated from E. wallichii.</p>
Assuntos
Arecaceae , Química , Medicamentos de Ervas Chinesas , Química , Furanos , Química , Glucosídeos , Química , Glicosídeos , Química , Fenilpropionatos , QuímicaRESUMO
A new compound and twelve known compounds were isolated from the ethyl acetate extract of the roots of Homonoia riparia Lour, which are used in folk medicine for treatment of hepatitis, bellyache and scald, by the method of silica gel column chromatography repeatedly with a gradient of PE-EtOAc, PE-Me2CO, CHCl3-Me2CO, CHCl3-MeOH. Their structures were identified as a new compound 1-oxo-aleuritolic acid (1), and twelve known compounds aleuritolic acid (2), 3-acetoxy-aleuritolic acid (3), taraxerone (4), taraxerol (5), methyl 3-acetoxy-12-oleanen-28-oate (6), 3-acetoxy-12-oleanen-28-ol (7), ursolic acid (8), lupenol (9), 3beta-acetoxy-lupenol (10), cleomiscosin A (11), chrysophanol (12), and gallic acid (13), which were obtained from this plant for the first time, by the spectroscopic techniques of NMR, HMBC, IR and MS, separately. Among the cytotoxicities evaluation of compounds 1 -3 towards AGZY 83-a (human lung cancer cells) and SMMC-7721 (human liver cancer cells) tumor cells was assayed by MTT methods with cis-dichlorodiamminoplatinum (DDP) used as positive control. Compound 2 exerted weak activity against AGZY 83-a with the IC50 value of 33.055 microg x mL(-1), while 1 and 3 showed no activity to these two cell lines.
Assuntos
Humanos , Antineoplásicos Fitogênicos , Química , Farmacologia , Linhagem Celular Tumoral , Sobrevivência Celular , Cumarínicos , Dioxanos , Química , Farmacologia , Euphorbiaceae , Química , Concentração Inibidora 50 , Estrutura Molecular , Ácido Oleanólico , Química , Farmacologia , Ácidos Palmíticos , Química , Farmacologia , Raízes de Plantas , Química , Plantas Medicinais , Química , Triterpenos , Química , FarmacologiaRESUMO
<p><b>OBJECTIVE</b>To study the effects of soil microbes of habitats on the cuttings rooting of Huperzia serrata and its mechanism.</p><p><b>METHOD</b>The cuttings of H. serrata were planted on the primary habitat soil and on the soil autoclaved, rooting rates were examined respectively. Changes of contents of phenolic compounds (catechin, quercetin, chlorogenic acid, ferulic acid) and indoleacetic acid (IAA) , and changes of activities of polyphenoloxidase (PPO) and peroxydase (POD) during the cuttings rooting were determined.</p><p><b>RESULT</b>Soil microbes of primary habitat could increase the rooting rate and the number of roots, could increase contents of catechin, chlorogenic acid, ferulic acid and IAA during rooting, and could affect activities of PPO and POD.</p><p><b>CONCLUSION</b>During the rooting higher contents of catechin, chlorogenic, ferulic acid and IAA caused by soil microbes of primary habitat are important factor for rooting rate.</p>
Assuntos
Catecol Oxidase , Metabolismo , Flavonoides , Metabolismo , Huperzia , Metabolismo , Microbiologia , Ácidos Indolacéticos , Metabolismo , Peroxidase , Metabolismo , Raízes de Plantas , Metabolismo , Microbiologia , Plantas Medicinais , Metabolismo , Microbiologia , Microbiologia do SoloRESUMO
<p><b>OBJECTIVE</b>To study the chemical constituents of the root of Paeonia sinjiangensis.</p><p><b>METHOD</b>The constituents were isolated by silica column chromatography, and their structures were identified on the basis of spectral analysis and their physical-chemical constants.</p><p><b>RESULT</b>Five compounds, paeoniflorin( I ), albiflorin (II), lactiflorin(III), daucosterol(IV), sucrose (V), were obtained.</p><p><b>CONCLUSION</b>All of the compounds were obtained from this plant for the first time.</p>
Assuntos
Benzoatos , Química , Hidrocarbonetos Aromáticos com Pontes , Química , Glucosídeos , Química , Glicosídeos , Química , Conformação Molecular , Estrutura Molecular , Monoterpenos , Química , Paeonia , Química , Raízes de Plantas , Química , Plantas Medicinais , QuímicaRESUMO
<p><b>OBJECTIVE</b>To reveal the pharmacological activities of the components for their further utilization and development by studying the chemical constituents of Citrus changshan-huyou.</p><p><b>METHOD</b>The structures were determined by repeated silica gel chromatographic separation and spectral analysis.</p><p><b>RESULT</b>Five compounds were obtained, and identified as 3-oxo friedelin (I), limonin (II), beta-sitosterol (III), 8-(2',3'-dihydroxy-4'-methylbutane)-7-methoxycoumarin (IV), sucrose (V).</p><p><b>CONCLUSION</b>The five compounds were obtained from this plant for the first time.</p>