Application of DNA methylation in detection of endometrial carcinoma in women with abnormal uterine bleeding at childbearing age / 中华检验医学杂志

Objective:This work aims to explore the application value of cervical exfoliated cell DNA (Cysteine dioxygenase type 1, CDO1 and CUGBP Elav-like family member 4, CELF4) methylation in the detection of endometrial cancer in women of childbearing age. Methods:From November 2021 to October 2022, a prospective study was conducted on a total number of 517 reproductive-age women with abnormal uterine bleeding who had surgical indications for hysteroscopy at the Xiangya Third Hospital of Central South University. The cervical exfoliated cells were collected for cytology, HPV (human papillomavirus) and gene methylation detection before operation. Clinical information of patients, level of tumor-related biomarkers, and endometrial thickness of transvaginal ultrasound (TVS) were also collected. Single factor regression method was used to analyze the high-risk factors of endometrial cancer. Receiver operating characteristic curve analysis was used to obtain the area under the curve(AUC), focusing on the screening efficacy of gene methylation test for endometrial cancer in women of childbearing age.Results:The age, body mass index (BMI)≥25 kg/m 2, endometrial thickness≥11 mm, CDO1 m ΔCt≤8.4, CELF4 m ΔCt≤8.8, and double gene methylation were associated with endometrial cancer in women of childbearing age, 1.16(1.08-1.25), 4.33(1.89-10.31), 9.49(3.88-26.69), 69.62(25.70-224.36), 23.64(9.66-63.99), 87.39(24.83-555.05), all P<0.05. The AUC was 0.90 (95% CI 0.83-0.97) of CDO1 m/ CELF4 m in diagnosing endometrial carcinoma was higher than others factors, with sensitivity and specificity of 91.7% (95% CI 80.6%-100%) and 88.8% (95% CI 86.0%-91.6%). TVS combined with DNA methylation detection further improved the sensitivity to 95.8% (95% CI 87.8%-100%), but could not improve the specificity 68.0% (95% CI 63.8%-72.1%). Conclusions:For women of childbearing age with abnormal uterine bleeding or abnormal vaginal discharge, the accuracy of cervical cytology DNA methyl detection of endometrial cancer is better than other non-invasive clinical programs. DNA methylation combined with TVS can improve the sensitivity of detection.

Health safety of the offspring after orthotopic fetal ovarian allotransplantation in rats / 中南大学学报(医学版)

OBJECTIVE@#To investigate the health safety of the offspring delivered following natural pregnancy after orthotopic fetal ovarian allotransplantation in rats.@*METHODS@#Any symptoms of spontaneous abortion during pregnancy and of any possible still birth and death of infant rats within 3 days after the delivery were observed and compared in 19 pregnant rats (the study group) after the orthotopic fetal ovarian allotransplantation and in another 10 pregnant rats (the control group). Forty offspring rats from each group were selected randomly. The mean weight at day 35 after the birth of offsprings was measured and compared. By routine G-banding technique, the karyotype was analyzed and the chromosomal number and structure were observed.@*RESULTS@#There was no spontaneous abortion, still birth, or death in the infants within 3 days after the birth in both groups. The body weight of offsprings at 35 days in both groups was (93.80 ± 4.93) g and (94.13 ± 4.53) g, respectively. There was no significant difference between the 2 groups (P> 0.05). The karyotype analysis indicated that the karyotype from both offspring groups was 42, XX or 42, XY. No chromosome abnormality (abnormal chromosomal number or abnormal chromosomal structure) was observed in both groups.@*CONCLUSION@#The health status of the offsprings of rats after orthotopic fetal ovarian allotransplantation is safe.

Laparoscopy Combined With Hysteroscopy Applied to Diagnose and Treat the Ovulatory Infertile Female / 中国医师杂志

Objective To assess the value of laparoscopy combined with hysteroscopy in the diagnosis and treatment of the ovulatory infertile female.Methods From Jan 2000 to May 2001, 140 ovulatory infertile patients were investigated and treated by laparoscopy combined with hysteroscopy. Findings of both the laparoscopy and hysteroscopy were compared with those of hysteroscopy alone and laparoscopy alone. Operative time, postoperative complications and days of hospitalization were recorded. Postoperative pregnancy outcomes were followed-up.Results This study demonstrates that combination of laparoscopy and hysteroscopy was superior to hysteroscopy alone and laparoscopy alone in the diagnosis of the ovulatory infertile female. It is short-time consumed, less traumatic, rapidly recovered. The postoperative pregnancy outcomes were satisfactory.Conclusions Laparoscopy combined with hysteroscopy is recommended as the primary precedure to the diagnosis and treatment of the ovulatory infertile female currently. It can cure some patients and provide reliable answer to whether assisted reproductive technique is needed as well.