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BACKGROUND: The prescriptions of multi-component have been the subject of chemical study fora long time. Therefore, when compounding the preparations of multi-component in traditionalmedicine, their taste is cautiously relied on, as the power of the medicine should not be subduedwith the power of another. Our research group has been carrying out tests on the raw materials,which are contained in multi-component prescriptions. However, research on multiple prescriptionsis relatively less being carried out.The traditional medicine naro-3 is used in traditionalmedical practicefor the treatment of inflammationand as a pain relieving remedy. Naro-3 is composed of 3 medicinal herbs including AconitumKuznezoffii Reichb, Terminalia chebula Retz, Piper longum L.GOAL: The aim of the study is to investigate some phytochemical compounds of traditional prescriptionNaro-3.OBJECTIVES:1. To reveal biological active substances of naro-3;2. The sum of the quantitative chemical study by spectrophotometry;3. To establish main criteria of standardMATERIALS AND METHODS: Traditional medicine narî-3 was produced from the Traditional Medicinefactoryof TMSTPC. In the phytochemical research, biological active substances were determinedby thin layer chromatography (TLC), on silica gel plats. The total contents of alkaloid and tannincompounds were determined by titrimetric method. TheMongolian pharmacopoeia was used fordetermination of quality parameters of traditional medicine Naro-3.RESULTS: TLC measurements of biological active substances of naro-3 showed that contains gallicacid and alkaloids respectively. The result of our research it was determined that the total tannin10.4 percent, total alkaloid 2.47 percent and organic acid 2.67 percent in drug Naro-3.CONCLUSION: The results of the study indicate that naro-3 contains a large amount of biologicalactive substances such as tannins, alkaloids and organic acids, which are connected with its painrelieving and anti-inflammatory effects.
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IntroductionNowadays the risk factors for thrombosis include blood stasis, vessel wall injury, and hypercoagulability, as proposed by Virchow over 150 years ago. We chose to study affect of Zomoshin 6 tan to the model of thrombosis formed in experimental animal. It was written in books and sudar that it has action of treating some type’s disease such as blood diluting, meeting frozen blood and treating some gynecological disease.PurposeTo study affect of Zomoshin-6 tan deep vein thrombosis model formed in experimental animal.Material and Methods30 male rats of wistar bread with 180-220 gram of weight for control group, experimental group or Zomoshin-6 tan and comparative group Warfarin. Thirty rats were equally divided into 3 groups: Group 1 received saline alone, Group 2 received Zomoshin-6 (200 mg/kg), and Group 3 received Warfarin as a positive control (0.25 mg/kg), seven days prior to the assessment of thrombus formation. Thrombus formation was also assessed histopathologically. Thrombi were detected in all rats after experimentallyinduced thrombosis.ResultsHistological analysis demonstrated the presence of thrombosis in the interior vena cava (IVC) of the control group, which contained fibrin, erythrocytes, and leucocytes and obstructed the lumen. Only a small amount of fibrin clot, containing a few leucocytes and large numbers of erythrocytes, were observed in the Zomoshin-6-treated group. The thrombus formed in the IVC of Warfarin-treated animals consisted of fibrin clot, which was mostly attached to the wall, with few leucocytes but abundant erythrocytes. These findings suggest that Zomoshin-6 is an effective antithrombotic agent.Conclusion:Zomoshin-6 tan has an action of inhibing thrombosis forming of vein in experimental animal.
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Introduction: The present work was focused on the investigation of resistance of biological membranes of various herbal formulations “Adapton 6” containing of rosa cinnamomea (плоды шиповника), Crataegus sanguine Pall (боярышника), Hippophae rhamnoides L (облепихи), radix Astragalus dasyanthus Pall (корни Астрагала) (левзеи) and Rheum palmatum L (ревеня).Purpose: study of evaluation of the membranostabilizing effect of “Adapton 6”Materials and Methods: in the experiment was made from 1% suspension of cleaned erythrocytes. The hemolisis of erythrocytes was induced by Phentona (peroxide hemolise) and added distilled water (osmotic hemolisis).Result: The membranostabilzing effect defined in vitro, according to the degree of bracing of peroxide and osmotic hemolisis of erythrocytes of donor’s blood 4. Suspension erythrocytes were taken with equal volume of distilled water to get osmotic hemolisis. The studied range of concentration of membranostabilizing effect of “Adapton 6” was 0.1 mkg/ml and reducing hemolisis in 64%, comparison with control group. In the studied drug were 1.0, 0.1and 0.01 mg/ml when degree of hemolisis reduced on 42. 52 and 60%, accordingly. The peroxide hemolise established that IC50 is 0.492 mg/ml (492 mkg/ml). Also in the studied drug were 1.0, 0.1 and 0.01 percent mg/ml when degree hemolisis reduced on 32, 49 and 51 respectively. The osmotic hemolise established that IC 50 is 0.6 mg/ml (60 mkg/ml). Conclusion: On conclusion, the prepared multicomponent herbal drug “Adapton 6” has membranostabilzing effect due to reduce of peroxide and osmotic hemolisis on the erythrocytes and degranulation adipocyates.
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Background: Wound healing mechanisms still unclear. Wounds are physical injuries that result in an opening or breaking of the skin. Proper healing of wounds is essential for the restoration of disrupted anatomical continuity and disturbed functional status of the skin. This is a product of the integrated response of several cell types to injury. Wound healing is a complex process that results in the contraction and closure of the wound and restoration of a functional barrier.Goal: The purpose of this study was to determine burn wound healing activity of Calvacin. Materials and Methods:We used in study as colored reaction thin layer chromatography (TLC) and UV-spectrophotometer methods to determine active compounds in the three component drug "Calvacin".In research were inducted 20 healthy white mice and 40 rats. Investigation was based and implemented at scientific research laboratory of Traditional Medical Science Technology and Production Corporation and Institute of Veterinary medicine of pathological laboratory. The study protocol was approved by the Ethics Committee of the Ministry of Health.Results:Results of the phytochemical investigations showed that contained in the new drug "Calvacin" determined the presence of bioactive substances such as flavonoids, saponin, tannin, curcuminoids, organic acids and minerals. Substance was absorbed 25.7% in 40 grade ethanol, 27.1% in 70 grade, 22.2% in 96 grade and 19.6% in sterile water. We was established quality quantities of Calvacin in the drug amount summary flavonoids 1% and determined by UV spectrophotometer method. Proceeding from the absorption maximum of the substances analyzed the wave length of detection was determined as 370nm. In the result of mineral study of the "Calvacin", Ca
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To explore the characteristics of this Lonicera Altaica, the author and her colleagues have been involved in the isolation and structure elucidation on this plant. During the course of this work, we were studied the plant was using by X-ray fl uorescence method at the institute of Physics and Technology of the Mongolian Academy of Sciences (MAS). Based on the results of multiemelent analyses, it was evident that potassium (23227 ppm) was found mainly in the plant and Se (0.34 ppm) preferably in the straw. In this study, the fatty acid composition plant was investigated. The fatty acid profi le analysis of the oil was also carried out by Gas Liquid Chromatography. The oil has three main fatty acids: palmitic, oleic and linoleic acids, linoleic acid being the most abundant. The plant content of the oil showed that 97.75% is unsaturated. It`s high content of linoleic acid is of particular interest especially in the fi ght against antioxidant and atherosclerosis. From the result of the study it was determined the preparation has no toxicity. This preparation has an anti infl ammatory and diuretic activity comparing with the control group. In this study, the protective effect preparation from Lonicera Altaica on liver infl ammation and fi brosis induced by carbon tetrachloride (CCl4) has been investigated in rats. Preparation from Lonicera Altaica signifi cantly suppressed CCI4-induced hepatic necrosis and infl ammation, as determined by the serum enzymatic activities of alanine and aspartate aminotransferase and serum alkaline phosphatase levels. In the histopathological study we carried out liver tissues from each rat were dissected; fi xed in 10 % neutralbuffered formalin. Microscopic examination was conducted on four m thick H. & E. stained sections from paraffi nembedded specimens. Treatment with preparation from Lonicera Altaica attenuated the necro-infl ammation and fi brogenesis in the CCI4-induced hepatic fi brosis, and thus it might be effective as a therapeutic anti-fi brotic agent.