RESUMO
<p><b>OBJECTIVE</b>To investigate the effect of Xianzhen tablet (XZT, a Chinese patent compound recipe) on advanced glycosylation end products (AGEs) and mRNA expression of AGE-specific cellular receptor (RAGE) in renal cortex of diabetic rats in order to explore the mechanism of XZT in protecting kidney.</p><p><b>METHODS</b>The diabetic rat model with persistent hyperglycemic renal damage was reproduced by streptozotocin. Fluorescent assay and RT-PCR were used to determine the content of AGEs and expression of RAGE mRNA in renal cortex in model rats, which were treated with XZT and controlled by aminoguanidine (AG) administration.</p><p><b>RESULTS</b>The relative content of AGEs and RAGE mRNA expression in renal cortex of model rats 12 weeks after modeling were significantly higher than those in the normal group (P < 0.05), while those in model rats treated by XZT or AG were markedly lower than those in non-treated model rats (P < 0.05), the effect of the both groups showed insignificant difference (P > 0.05).</p><p><b>CONCLUSION</b>XZT could reduce the accumulation of AGEs in renal cortex of diabetic rats, down-regulate the over-expression of RAGE mRNA, with the effects similar to that of AG, the inhibition of XZT on protein non-enzymatic glycosylation might be one of the mechanisms of its effect in protecting kidney.</p>
Assuntos
Animais , Masculino , Ratos , Diabetes Mellitus Experimental , Genética , Metabolismo , Patologia , Nefropatias Diabéticas , Genética , Metabolismo , Patologia , Regulação para Baixo , Medicamentos de Ervas Chinesas , Farmacologia , Produtos Finais de Glicação Avançada , Genética , Córtex Renal , Metabolismo , RNA Mensageiro , Genética , Ratos Wistar , Receptor para Produtos Finais de Glicação Avançada , Receptores Imunológicos , Genética , ComprimidosRESUMO
<p><b>OBJECTIVE</b>To investigate the effect of Wenjingtong Composita (WJTC) on blood glucose and advanced glycosylation end products (AGEs) in sciatic nerve of streptozotocin (STZ)-induced diabetic rats.</p><p><b>METHODS</b>STZ-induced diabetic rats were randomized to WJTC prevention group and WJTC treatment group. The levels of blood glucose and AGEs in sciatic nerve of the animals were checked after 12 weeks treatment and compared with that of aminoguanidine (AG) treatment group.</p><p><b>RESULTS</b>Blood glucose level in the WJTC prevention and treatment group, and AGEs in sciatic nerve of the WJTC prevention group and the AG group were lower than those of the non-treated group (P < 0.01). Blood glucose level in the AG group was higher than that in the WJTC prevention group (P < 0.05), but was not significantly different from that in the non-treated group (P > 0.05).</p><p><b>CONCLUSION</b>WJTC might prevent diabetic peripheral neuropathy by decreasing blood glucose and inhibiting AGEs formation in sciatic nerve in STZ-induced diabetic rats.</p>