Analysis on application of urine protein electrophoresis in renal diseases / 国际检验医学杂志

Objective To explore the application value of non‐concentrated urine protein electrophoresis in renal diseases in order to guide to adjust the treatment scheme and judge the prognosis .Methods The clinical data in 152 cases of renal biopsy in the Xuzhou Municipal First People′s Hospital from December 2010 to January 2016 were retrospectively collected ,mainly including the pathological report and the results of urine protein electrophoresis .The classification was performed according to the WHO renal pathology classification standard(Ⅰ ,Ⅱ ,Ⅲ ,Ⅳ) .The relationship between the renal pathological classification with the classification of urine protein electrophoresis and the proportions of various compositions in electrophoresis spectrum was comparatively ana‐lyzed .Results The glomerular lesion rate was higher than the renal tubules‐interstitial lesion rate ,the majority of renal tubules‐in‐terstitial lesion had combining glomerular lesions .The glomerular urine protein level reflected by the urine protein electrophoresis was associated with glomerular damage degree ,with WHO pathological grade (Ⅰ - Ⅳ ) increase ,the proportion of haptoglobin (HAP)and IgG/A in urine protein electrophoresis showed an increasing trend ,the differences were statistically significant (F=2 .237 ,P<0 .05 ;F=7 .269 ,P<0 .05) .Conclusion Detecting the proportion of HAP and IgG/A in urine protein electrophoresis has an important clinical value in reflecting the glomerular damage severity .

Analysis of drug susceptibility test results of Pseudomonas aeruginosa in Xuzhou area / 国际检验医学杂志

Objective To understand the drug resistance characteristics of pseudomonas aeruginosa and to guide the selection of clinical antibacterial drugs .Methods The clinically isolated 335 strains of pseudomonas aeruginosa from the Xuzhou Municipal First People′s Hospital in 2013 were collected and performed the drug susceptibility test .The test results and data were analyzed by using the WHONET5 .6 software .Results Clinically isolated 335 strains of pseudomonas aeruginosa had different degrees of re‐sistance to 15 kinds of antibacterial drugs ,in which the sensitivities to piperacillin/tazobactam ,amikacin ,tobramycin ,ciprofloxacin , levofloxacin ,ceftazidine ,cefepime and gentamicin were higher ;the resistance rate to ampicillin/sulbactam ,ceftriaxone ,ampicillin and cefotaxime was up to above 97% .In the same patients with several times of this bacterial detection ,larger volatility existed in the susceptibility results of piperacillin/tazobactam ,ceftazidine ,cefepime ,imipenem and gentamicin .Meanwhile the drug resitance spectrum of Pseudomonas aeruginosa had a certain tendency .Conclusion Strengthening continuous etiology and drug resistance moni‐toring is in response to the occurrence of acute drug resistance and nosocomial infection spread ,meanwhile guides clinical treatment .

Transient Receptor Potential Vanilloid Type-1 Channel in Cardiometabolic Protection

Transient receptor potential vanilloid type-1 channel (TRPV1) is a non-selective cation channel with a preference for calcium ions that is able to sense a vast range of endogenous physical and chemical stimuli and plays an important role in transducing the sensations of noxious heat and pain signaling. Recent studies showed that TRPV1 is widely expressed in different tissues and organs beyond the sensory nerves and has multiple biological effects that are involved in functional regulation in the pancreas, blood vessel, adipose tissue and liver. To further understand the link between TRPV1 and cardiometabolic diseases, we reviewed the role of TRPV1 in hypertension, diabetes, obesity, and dyslipidemia. This review provides new insights into the involvement of TRPV1 channels in the pathogenesis of cardiometabolic disorders and implicates this channel as a potential therapeutic target for the management of cardiometabolic diseases.

Capsaicin Inhibits Angiotensin Ⅱ Induced Vasoconstriction in Mice / 中华高血压杂志

Objective To study the long-term effect of administration(6 months) with transient receptor potential vanilloid 1(TRPV1) agonist capsaicin on contractile reactivity of thoracic aorta in C57BL/6J mice.Methods Tow-month-old male C57BL/6J mice were received normal diet group(n=12) or capsaicin group(normal diet plus capsaicin,n=12).Tail-cuff systolic blood pressure(SBP) was examined at the baseline and at the end of the intervention.After 6-month treatment period,carotid artery blood pressure and heart rate were determined by catheterization,and the aortic contractile response was examined using isometric myograph(Danish Myotech Technology,Denmark).Plasma levels of renin,angiotensin Ⅱ(Ang Ⅱ) and aldosterone were determined.Vascular smooth muscle cells(VSMC) were obtained from thoracic aorta of mice and cultured.Angiotensin Ⅱ type 1 receptor(AT1R) protein expression was detected by western blot.Calcium imaging was detected in cultured VSMC using the fluorescent dye technique.Results Systolic blood pressure,invasive carotid artery blood pressure and heart rate have no difference between two groups.No differences was found in PE-induced contraction response in thoracic aorta;while Ang Ⅱ induced contractility of aortic ring was lower in mice with capsaicin than control group [capsaicin:(37.5?1.6)% vs(59.8?1.4)%,P

Cardiovascular and Renal Damage in Hypertensives Complicated with Metabolic Disorders / 中华高血压杂志

Objective To study the target organ damage in patients of hypertension with metabolic disorder. Methods 1033 patients were divided into five groups: hypertension without complications(102 cases), hypertension with dyslipidemia (117 cases), hypertension with abdominal obesity(119 cases), hypertension with type 2 diabetes mellitus(135 cases), hypertension with metabolic syndrome (560 cases). The structure and the function of heart and blood vessel were examined by color Doppler. Urinary microalbuminuria was determined. Results (1)Only 9.9% patients of hypertension were without metabolic disorder, 90.1% hypertensives complicated with the metabolic disorder; (2)Compared with hypertension with dyslipidemia, hypertension with type 2 diabetes mellitus (P90%) complicated with the metabolic disturbance. Hypertension complicated with abdominal obesity accentuate left ventricular hypertrophy. Hypertension with diabetes mellitus and metabolic syndrome accentuated the vascular and renal lesion.

Study on the relationship between insulin resistance and type 2 diabetes mellitus with postural hypotension / 第三军医大学学报

Objective　To investigate the occurrence of postural hypotension (PH) in patients suffering from type 2 diabetes mellitus with or without hypertension (DMH or DM), and the relationship of PH and diabetic neuropathy, hyperinsulinemia and insulin resistance. Methods　A total of 30 cases of type 2 DM and 30 cases of DMH were included in this study. The blood pressure of all subjects were measured in supine and standing body positions respectively and PH was defined as a decline from supine to standing was ≥20 mmHg in systolic blood pressures (SBP). The concentrations of blood glucose and plasma insulin were measured to calculate the insulin sensitive index (ISI). Autonomic and peripheral function was determined by measuring the postural heart rates and the conduction speeds of superficial peroneal and communicating branch of peroneal nerves etc respectively. Results　Significant difference (P<0.01) was found in the occurrence of PH in the patients with DM (40%) and those with DMH (67%). The changes of postural blood pressure were more obvious in those with DM+PH and DMH+PH than in those with simple DM (P<0.01). The conduction speeds of newes were significantly lower in those with DMH+PH than with simple DM (P<0.05), but the occurrence of autonomic neuropathy had no difference between the 2 groups. There was no difference in postural heart rate, body mass index and blood glucose levels in fasting and 2 h after meal among the DM, DM+PH and DMH+PH groups. The concentrations of plasma insulin of those with DMH+PH were significantly higher, but their ISI significantly lower than those of the patients with DM respectively (P<0.01). The decline of postural SBP in patients with DMH+PH had a significantly positive correlation with their plasma insulin levels in fasting condition (r=0.689, P<0.01). Conclusion　The patients with DMH are more prone to PH compared with those only with DM and PH damages their peripheral nerves. Most of diabetic patients with PH suffer from obvious IR and hyperinsulinemia, and if with hypertension, the above metabolic disturbances are more severe.