RESUMO
Human cord blood-derived stem cells are a rich source of stem cells as well as precursors. With regard to the researchers have focused on the therapeutic potential of stem cell in the neurological disease such as stroke, the aim of this study was the investigation of the therapeutic effects of human cord blood-derived stem cells in cerebral ischemia on rat. This study was carried out on young rats. Firstly, to create a laboratory model of ischemic stroke, carotid artery of animals was occluded for 30 minutes. Then, umbilical cord blood cells were isolated and labeled using bromodeoxyuridine and 210[5] cells were injected into the experimental group via the tail vein. Rats with hypoxic conditions were used as a sham group. A group of animals did not receive any injection or surgeries were used as a control. Obtained results were evaluated based on behavioral responses and immunohistochemistry, with emphasis on areas of putamen and caudate nucleus in the control, sham and experimental groups. Our results indicated that behavioral recovery was observed in the experimental group compared to the either the sham or the control group. However, histological studies demonstrated a low percent of tissue injury in the experimental group in comparison with the sham group. Stem cell transplantation is beneficial for the brain tissue reparation after hypoxic ischemic cell
RESUMO
This study aimed to compare urinary Tamm-Horsfall protein [THP], citrate, and other inhibitors and promoters of stone formation in calcium stone formers with those in healthy individuals. From January 2002 to June 2004, 100 calcium stone formers [mean age, 38.6 +/- 10.3 years] who had at least 2 episodes of calcium stone formation were compared with 100 healthy individuals [mean age, 33.8 +/- 9.7 years]. Their 24-hour urine THP [using the sodium dodecyl sulfate polyacrylamide gel electrophoresis method], citrate, calcium, uric acid, oxalate, and magnesium values were measured and compared. The mean 24-hour urine THP was 3.3 +/- 8.1 mg in patients in the study group and 4.6 +/- 19.2 mg in controls [P=0.5]. However, THP in individuals with and without bacteriuria was significantly different [15.8 +/- 33.6 versus 2.6 +/- 10.2, P<0.001]. Mean 24-hour urinary calcium, citrate, and oxalate values were 232.6 +/- 95.3 mg and 177.8 +/- 82.7 mg [P<0.001], 132 +/- 103.2 mg and 395 +/- 258.5 mg [P<0.001], and 18.9 +/- 22.5 mg and 10.4 +/- 8.5 mg [P<0.001] in patients in the study and control groups, respectively. There was a significant positive correlation between urinary citrate and promoters of stone formation, including urinary calcium, oxalate, and uric acid, in patients in the control group, but not in patients in the study group. THP in the urine of stone formers is not quantitatively different from that of healthy individuals, but it is different in patients with bacteriuria. Increased urinary excretion of calcium, oxalate, and uric acid in stone formers with no increase in urine citrate may play a role in the pathogenesis of recurrent stone formation