Primary abdominal cocoon syndrome, situs inversus totalis and bilateral cryptorchidism: a case report / 中华泌尿外科杂志

This paper reviewed the clinical data of a patient with primary abdominal cocoon syndrome, situs inversus totalis and bilateral cryptorchidism admitted to our hospital in March 2021, and discussed the clinical characteristics of the disease based on the literature. This case is relatively rare, and all three diseases involve congenital abnormalities that may lead to developmental disorders in the embryo. The clinical manifestations of abdominal cocoon syndrome lack of specificity, preoperative diagnosis is difficult, often accompanied by partial dysplasia, so it is necessary to improve the awareness of preoperative differential diagnosis.

Characterization of inulosucrase and the enzymatic synthesis of inulin / 生物工程学报

As a type of prebiotics and dietary fiber, inulin performs plenty of significant physiological functions and is applied in food and pharmaceutical fields. Inulosucrase from microorganisms can use sucrose as the substrate to synthesize inulin possessing higher molecular weight than that from plants. In this work, a hypothetical gene coding inulosucrase was selected from the GenBank database. The catalytic domain was remained by N- and C- truncation strategies, constructing the recombinant plasmid. The recombinant plasmid was expressed in E. coli expression system, and after purifying the crude enzyme by Ni²⁺ affinity chromatography, a recombinant enzyme with a molecular weight of approximately 65 kDa was obtained. The optimal pH and temperature of the recombinant enzyme were 5.5 and 45 °C, respectively, when sucrose was used as the sole substrate. The activity of this enzyme was inhibited by various metal ions at different degrees. After purifying the produced polysaccharide, nuclear magnetic resonance analysis was used to determine that the polysaccharide was inulin connected by β-(2,1) linkages. Finally, the conditions for the production of inulin were optimized. The results showed that the inulin production reached the maximum, approximately 287 g/L after 7 h, when sucrose concentration and enzyme dosage were 700 g/L and 4 U/mL, respectively. The conversion rate from sucrose to inulin was approximately 41%.

IDENTIFICATION OF PLANT PATHOGENIC BACTERIUM FROM CROWNGALL OF JAPANESE CHERRY (PRUNUS XYROLOEUSIS) ANDCONTROL TECHNOLOGY / 微生物学通报

11 plant pathogenic strains were isolated from the crown gall of Japanese cherry (Prunus xyroloensis)in Japanese cherry growing areas, such as Chixing. fenhua and Shenzhou counties of Zhejiang province. Ten ofthem were identified as Agrobacterium tumefaciens biotype 1 based on their morphological. physiological andbiochemical characterizations and bacterial protein SDS-PAGE test. The plate antagonistic assay and the potbiocontrol test indicated that the isolated strains of A tumefaciens biotype 1 were sensitive to the well-knownantagonistic bacterium , K84 strain. The K84 strain could inhabit the tumor production by the pathogenic satins.