In Vitro Study of Adipose-Derived Mesenchymal Stem Cells Transduced with Lentiviral Vector Carrying the Brain-Derived Neurotrophic Factor Gene

Brain-derived neurotrophic factor (BDNF) exerts its survival-promoting effects on photoreceptors and retinal ganglion cells, however, delivery systems with little-to-no side effect are needed to sustain its controlled release and long-term efficacy. Our previous studies demonstrated that adipose-derived stem cells (ADSCs) are ideal delivery systems for gene therapy; moreover, ADSCs present unique properties like migration to damaged tissue sites, immunomodulation and anti-inflammation. Herein, we propose to employ ADSCs as the BDNF gene delivery vehicle. Different Analyses like flow cytometry, differentiation and cell proliferation assays etc demonstrated that BDNF were successfully transduced into ADSCs and the stemness of ADSCs was maintained even with the transduction. Real Time PCR and Western blot were used to measure mRNA and protein expressions of the BDNF-transduced ADSCs. The results demonstrated that the BDNF expression level of the lentiviral-BDNF transduced ADSCs is significantly increased and, moreover, enhanced the expression of other neurotrophic and downstream signaling factors. The data suggest that ADSCs are a reliable delivery vehicle for BDNF and could be used for the treatment of various diseases.

Epidemiological analysis of primary liver cancer in the early 21st century in Guangxi province of China / 癌症

<p><b>BACKGROUND AND OBJECTIVE</b>In Guangxi province, from 1970s to 1990s, the mortality of primary liver cancer (PLC) ranked the first among a variety of malignant tumors. Investigating the epidemiological characteristics of PLC is very important for developing reasonable and effective treatment strategy, allocating health resources rationally, and evaluating the quality of PLC prevention and control. This study was to analyze the mortality and epidemiological characteristics of PLC in Guangxi province between 2004 and 2005.</p><p><b>METHODS</b>Multi stage stratified cluster random sampling method was used to select 9 counties (cities or urban areas) as sample points. The residents' death causes between 2004 and 2005 were analyzed, and the epidemiological characteristics of PLC were investigated.</p><p><b>RESULTS</b>In the period of 2004-2005, the crude mortality of PLC was 34.39/100,000 in Guangxi province population (55.30/100,000 in men and 13.21/100,000 in women). The national population standardized mortality in 1964 was 22.17/100,000. The man to woman ratio of mortality was 4.19:1. PLC ranked as the first death cause among a variety of malignant tumors, and PLC related death accounted for 30.70% of all tumor related death cases. Age specific mortality of PLC was increased with age, rising significantly from 30 year old (from 25 year old in men and from 40 year old in women), and reached a peak at 75 year old.</p><p><b>CONCLUSIONS</b>The mortality of PLC shows a decreasing trend in Guangxi province in the early 21st century, and the starting age of PLC death peak postpones about 10 years than that in 1990s. It shows that the comprehensive prevention and control measures of PLC implemented in Guangxi province are fruitful. However, the PLC mortality in Guangxi province is still significantly higher than the national average level, and it still ranks as the first death cause in a variety of malignant tumors in Guangxi province. PLC mainly occurs in middle aged and elderly people. The prevention and treatment research of PLC still has a long way to go.</p>

Study on the relationship between familial clustering of hepatocellular carcinoma and polymorphism of cytochrome P450 2E1 gene in Zhuang populationl Guangxi / 中华流行病学杂志

Objective To study the relationship between familial clustering of hepatocellular carcinoma(HCC)and the polymorphism of cytochrome P450 2E1 gene(CYP2E1)as well as of other relevant risk factors to the cancer.Methods Peripheral blood samples were collected from 91 members of 10 HCC clustering families and 102 of 10 control families,among Zhuang population,in Guangxi.The area had been with high incidence rate of HCC.Genotypes and allele frequencies of CYP2E1 Rsa I site were determined by polymerase chain reaction.combined with restriction fragment length polymorphism method (PCR-RFLP).Serum HBsAg was tested by means of ELISA.Data on relevant risk factors of the cancer were collected as well.through a unique questionnaire.Results Frequencies of c1/c1 and c1/c2 genetypes of CYP2E1 Rsa I site were 63.7%and 36.3%.respectively,in the members of families with cancer clustering phenomena.In the members of the control families.these two rates were 48.0%and 52.0%,respectively (OR=1.901,95%CI:1.067-3.387).Difference of genotypes frequencies of CYP2E1 Rsa I site between the members in these two groups was statistically significant(X2=4.797,P=0.029).According to the results from non-condition logistic regression analysis.the major risk factors on familial clustering of HCC could be listed as:intake of corns.HBsAg carrying status and CYP2E1 c1/c1 genotype.Conclusion The relationship seemed to exist between familial clustering of HCC and the frequencies of polymorphism of cytochrome P450 2E1 gene(CYP2E1).The frequencies of CYP2E1 Rsa I site were neither the only nor the major factor,causing the familial clustering phenomenon of cancel More possible,it Was the affect of syntheses with the involvement of multiple factors.