Progress on molecular biology of Isaria farinosa, pathogen of host of Ophiocordyceps sinensis during the artificial culture / 中国中药杂志

Isaria farinosa is the pathogen of the host of Ophiocordyceps sinensis. The present research has analyzed the progress on the molecular biology according to the bibliometrics, the sequences (including the gene sequences) of I. farinosa in the NCBI. The results indicated that different country had published different number of the papers, and had landed different kinds and different number of the sequences (including the gene sequences). China had published the most number of the papers, and had landed the most number of the sequences (including the gene sequences). America had landed the most numbers of the function genes. The main content about the pathogen study was focus on the biological controlling. The main content about the molecular study concentrated on the phylogenies classification. In recent years some protease genes and chitinase genes had been researched. With the increase of the effect on the healthy of O. sinensis, and the whole sequence and more and more pharmacological activities of I. farinosa being made known to the public, the study on the molecular biology of the I. farinosa would be deeper and wider.

Effect of wound to growth of larva of host to Ophiocordyceps sinensis during artificial breeding / 中国中药杂志

To clear the effect of the wound to the growth of the larva of the host to the Ophiocordyceps sinensis, the wounds of same severity at the same position were made artificially to the larva and which were artificial fed at the same environment and condition. The results indicated that, over the winter, the survival rate of the wounded of the infection larva was lower than that of the healthy larva, but the weight had no significant difference between the wounded and the healthy larva. The survival rate of the wounded of the no infection larva was lower than that of the healthy larva, but except with black skin, the wounded larva with offwhite and dusty red had no influence on the variety of the weight. In summery, wound had no advantage to the survival rate, but had no influence to the weight. The result had provided theoretical basis to the reforming of the system of the artificial culture O. sinensis.

Effect of N, P, and K fertilizers on sclerotial formation and germination of southern blight of Coptis chinensis / 中草药

Objective: To study the effect of nitrogen (N), phosphorus (P), and potassium (K) fertilizers on the sclerotial formation and germination of southern blight of Coptis chinensis so as to provide scientific basis for its integrated pest management (IPM). Methods: Preparation of different concentrations of N, P and K was added into soil and the mycelium and sclerotia were put on the soil, and then the effect of the fertilizers on the sclerotial formation and germination was observed. Results: The treatment of CO(NH 2)2 and NH4HCO3 had significant inhibition on sclerotial formation and germination. The treatment of NaH 2PO4, KCl, and KNO3 showed the facilitation in various degrees to promote the sclerotial formation and germination. The treatment of KH2PO4 had no significant effect on sclerotial germination, but it had significant inhibitory activity to sclerotial formation. Conclusion: In the production, the partial N, while less K and P fertilizers should be put to control the sclerotial formation and germination of southern blight of C. chinensis.

Effects of slow release-fertilizers on yield, nutrient contents and quality of Coptis chinensis / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effects of four kind of slow-release fertilizers on yield and quality of Coptis chinensis.</p><p><b>METHOD</b>One to three years C. chinensis was fertilized with slow-release fertilizers twice in April and in September. The yield and nutrient content along with quality of C. chinensis were measured after two years growth.</p><p><b>RESULT AND CONCLUSION</b>All of the slow-release fertilizers increased the yield obviously, and the effect of SRF1 and SRF4 is the most significant. Comparing with control group, the N content in aerial part of 1-2 year-old C. chinensis treated with SRF1 and SRF4 was lower and P and K were higher than that of control group, and the N content in aerial part of 3 year-old C. chinensis treated with SRF1 and SRF4 was higher and P and K were higher than that of control group; The N content in the root of land 3 year-old C. chinensis treated with SRF1 and SRF4 showed no significant difference comparing with control group, and P and K were lower than that of control group, the N and P content in root of 2 year-old C. chinensis treated with SRF1 and SRF4 was higher and K were lower than that of control group. After two years growth berberine content of C. chinensis treated with SRF1, SRF2 and SRF3 were significantly increased, and total alkaloid content of C. chinensis treated with SRF1, SRF3 and SRF4 were significantly increased. We recommend that SRF4 is used as the special fertilizer for 1-year-old C. chinensis, and the SRF1 and SRF4 for 2-year-old C. chinensis, and the SRF1 for 3-year-old C. chinensis.</p>

Effects of integrated pest control techniques to growth of host larvae Cordyceps sinensis / 中国中药杂志

<p><b>UNLABELLED</b>To study the effects of the integrated pest control techniques on growth of host larvae of Cordyceps sinensis.</p><p><b>METHOD</b>The integrated pest control techniques were compared with conventional techniques to evaluate the effects on growth of host larvae.</p><p><b>RESULT AND CONCLUSION</b>The results showed that the techniques had broken the balance of the microbial living in the material, produced effective inhibition on the pests, raised the survival rate and promoted the growth of the host larvae at the same time.</p>

Clinical significance of adenosine 99mTc-MIBI myocardial perfusion single photon emission computed tomography for patients in percutaneous coronary intervention / 中华心血管病杂志

<p><b>OBJECTIVE</b>To analyze the clinical significance of adenosine (99m)Tc-MIBI myocardial perfusion single photon emission computed tomography (SPECT) in patients with coronary artery disease (CAD) for percutaneous coronary intervention (PCI).</p><p><b>METHODS</b>Coronary angiography and adenosine (99m)Tc-MIBI myocardial perfusion SPECT were performed for all patients. Adenosine myocardial perfusion was performed after PCI. Adenosine was infused intravenously at a rate of 140 microg.kg(-1).min(-1) for 6 minutes, and 925MBq (99m)Tc-MIBI was injected intravenously at 3 minutes after adenosine infusion. SPECT myocardial imaging acquisition was obtained in 1.5 hours after adenosine infusion. If the result was abnormal, rest (99m)Tc-MIBI myocardial perfusion SPECT would be performed next day. There were 17 segments of left ventricle, and four degrees of myocardial perfusion.</p><p><b>RESULTS</b>There were 63 cases (63 +/- 10 years old) with CAD, in which 40 patients got PCI. Twenty eight cases after PCI.</p><p><b>CONCLUSION</b>Adenosine myocardial perfusion imaging will be useful in detecting regional myocardial perfusion abnormalities for patients with PCI.</p>