Effects of Triptolide on Tc and Th Cell Excursion in Peripheral Blood of Nude Mice with Systemic Lupus Erythematosus BALB/c-un / 中国实验血液学杂志

OBJECTIVE@#To investigate the effect of triptolide on the excursion of Tc and Th cells in peripheral blood of systemic lupus erythematosus (SLE) BALB/c-un nude mice induced by pristane.@*METHODS@#Eighteen female BALB/c-un nude mice were randomly divided into blank, SLE and triptolide group, each with 6 mice by random table method. Group SLE and group triptolide were established by single intraperitoneal injection of pristane, and blank group was used as blank control group. SLE model was established by single intraperitoneal injection. Triptolide group was fed with triptolide at the dose of 5 mg/(kg·d), and the blank group and SLE group were fed normally. Blood samples were collected from the caudal vein before treatment and 1, 3 and 6 months after treatment respectively. Fluorescence labeled flow cytometry was used to delect Tc and Th lymphocyte subsets at different stages of treatment.@*RESULTS@#After treatment for 3 and 6 moths, the percentages of Tcl, Thl cells and CD8, Tcl/Tc2, Thl/Th2 and CD4/CD8 all decreased in the group of triptolide, and the percentage of CD4, Tc2 and Th2 cells increased (P＜0.05).@*CONCLUSION@#The mechanism of triptolide in the treatment of SLE may be related with the excursion of Tc and Th cells to Tcl and Tc2 to maintain the relative homeostasis of Tc and Th cells at different stage, thus affecting the immune response and the inflammatory reaction.

Effect of Quercetin on the Cell Cycle and Adhesion Molecules of NOD/SCID Mice with Acute B Lymphocytic Leukemia / 中国实验血液学杂志

OBJECTIVE@#To investigate the effect of Quercetin on cell cycle and adhesive molecules of NOD.SCID mice with acule B lymphocytic leuaemia(B-ALL).@*METHODS@#5×10 Nalm-6(B-ALL cell line) cells were injected into the tail vein of 48 NOD/SCID mice to establish the NOD/SCID mice with B-ALL. After 15 day, the NOD/SCID mice with B-ALL were randomly divided into 3 groups: salive group as control (injection with saline of 0.2 ml/mouse), cyclophos-phamid group (injection with cyclophosphamide of 100µg/kg) and quercetin group(injection with quercetin of 3 mg/kg). After treatment for 21 d, the perecntage of Nalm-6 cells in G1, G2, M and S phases was detected by flow cytonetry; the B lymphocytes Nalm-6 cells, neutrophils and WBC in while blood were counted before and after treatment; the expression of intercellalar. Adhesion molecole-1(FCAIU-1), vascular cell adhesion molecule-1(VCAM-1) and P-selectin was detected by double autibody soundwich method.@*RESULTS@#Compared with level before treatment, the expression of ICAM-1, VCAM-1 and P-selectin decreased after treatment with guercetin, The hemogram showed that the peripheral blood nentrophil level obviously increased, while the levels of B lymphocytes, Nalm-6 cells and WBC count decreased obviously after treatment with guercetin. The cell proliferatim rario in G0/G1 phase decreased, yet the cell proliferation ratio in S and G2/M phases increased after treatment with guercetin.@*CONCLUSION@#The guercetin can decrease the intercellular adhesion through inhibition of ICAM-1 expression, and arrests Nalm-6 cells in S and G2/M phases. The guercetin has obviously inhibitory effect on B-ALL cells.

Effects of Epihopin on Proliferation and Apoptosis of KG-1a cells and Its Related Mechanism / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To explore the possible mechanism underlaying interference of epihopin on the proliferation of AML KG-1a cells by inhibiting the Wnt/β-catenin signaling pathway, so as to prvide the experimental basis for development of drug to treat the AML.</p><p><b>METHODS</b>A total of 50 c57BL/6 mice were randomy divided into 5 group:blank control, model control, high, medium and low dose of epihopin. Except the blank control group, the KG-1a cells were injected in abdominal cavity of 4 groups for the establishment of model. The mice in high, middle and low dose groups were injected intramuscularly with 80, 40 and 20 mg/kg of epihopin respectively, while the mice in blank control and model control group were injected intramuscularly with saline. The Western blot was used to detect the expression of S phase kinase-related protein 2(SKP-2), β-catenin, E-cadherin and poly-(ADP ribose) polymerase (PARP); the spectrophotometry was used to detect the activity of caspase 3 and procaspase-3, the flow cytometry was used to detect the cell cycle distribution and the apoptotic rate of KG-1a cells treated with epihopin.</p><p><b>RESULTS</b>The epihopin could enhance the activity of caspase 3, decrease the level of procaspase 3; also could up-regulate the expression of E-acadherin and down-regulate the expression of SKP-2 and β-catenin; and could increase the expression of PARP in dose-dependent manner. After KG-1a cels were treated with epihopin, the apoptosis rate of cells significantly increased, the KG-1a cells were arrested in G/G phase, therefore the growth of KG-1a cells was significantly inhibited.</p><p><b>CONCLUSION</b>The epihopin can dose-dependently split PARP to induce the apoptosis of KG-1a cells, its mechanism may relate with inhibition of Wnt/β-catenin signaling pathway and its down-stream-related gene expression.</p>