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1.
Chinese Journal of Contemporary Pediatrics ; (12): 263-267, 2014.
Artigo em Chinês | WPRIM | ID: wpr-269494

RESUMO

<p><b>OBJECTIVE</b>To investigate the effects of different tilt angles of head-up tilt test (HUTT) and different responses to HUTT on the psychological fear in children undergoing the test.</p><p><b>METHODS</b>HUTT was performed on children with unexplained syncope or pre-syncope (107 cases: 52 males and 55 females), aged 5.5-17.8 years (mean 12.0±2.8 years). All subjects were randomly assigned to undergo HUTT at an angle of 60°, 70° or 80°; the negative cases underwent sublingual nitroglycerin-provocation HUTT at the same tilt angle. The Wong-Baker Faces Pain Rating Scale was used for self-assessment of psychological fear in subjects during HUTT at the end point of the test.</p><p><b>RESULTS</b>The positive rate, hemodynamic changes and distribution of response types showed no significant differences between children at tilt angles of 60°, 70° and 80° (P>0.05). The greater the tilt angle, the higher the degree of psychological fear in children undergoing the test, but there were no significant differences between them (P>0.05). The degree of psychological fear in children who showed a positive response to HUTT (n=76) was significantly higher than that in children who showed a negative response (n=31) (P<0.01).</p><p><b>CONCLUSIONS</b>HUTT can cause psychological fear in children undergoing the test, and the degree of psychological fear increases in children tested at tilt angles from 60° to 80°, but the differences have no statistical significance. A positive response to HUTT can significantly increase the psychological fear in children.</p>


Assuntos
Adolescente , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Medo , Teste da Mesa Inclinada , Psicologia
2.
China Journal of Chinese Materia Medica ; (24): 27-30, 2008.
Artigo em Chinês | WPRIM | ID: wpr-324306

RESUMO

<p><b>OBJECTIVE</b>To study the formulation and preparation of ampelopsin liposomes and evaluate their quality.</p><p><b>METHOD</b>The liposomes were prepared by a film-ultrasonic dispersion technique. Served as quota with the entrapment ratio and appearance and diameter of the liposomes, the optimal formulation and preparation were selected by means of an uniform design test. The appearance of liposomes was observed by micrography. The diameter and electric charge of surface were determined by granularity mensuration instrument. The entrapment ratio and the leakage rate of ampelopsin liposome were determined by means of dialyze. The content of ampelopsin was determined by UV.</p><p><b>RESULT</b>The result of electron micrography and the size distribution showed that the liposomes were similar to spherical small unilamellar vesicles. The mean diameter was (258.2 +/- 51.2) nm and the electric charge of surface is 19.0 mV. The entrapment ratio of ampelopsin liposomes was 62. 3% and the lecithoid oxidative rate was 0.83% (n = 3).</p><p><b>CONCLUSION</b>The selected formulation and preparation of ampelopsin liposomes is efficient and practicable.</p>


Assuntos
Flavonoides , Química , Lipossomos , Química , Microscopia Eletrônica
3.
National Journal of Andrology ; (12): 883-887, 2006.
Artigo em Chinês | WPRIM | ID: wpr-289118

RESUMO

<p><b>OBJECTIVE</b>To find the expression of specific genes related to the meiosis of germ cells during spermatogenesis in the rat testis.</p><p><b>METHODS</b>Segments of seminiferous tubules were obtained from the adult male SD rats, at stages XIII - I of meiosis, and the interstitial cells of the same testis were isolated under the stereomicroscope. The total RNAs of stages XIII - I segments and the testicular interstitial cells were extracted respectively, and mRNA differential display RT-PCR (DDRT-PCR) was conducted. The obtained cDNA fractions were purified and recovered, the reverse dot blot hybridization, sub-clones and screens of blue/white dots performed, and the results of sub-clones were identified by restriction endonuclease EcoR I digestion.</p><p><b>RESULTS</b>Sixteen differential cDNA fractions were obtained through primary DDRT-PCR, 7 from stages XIII - I segments and 9 from the testicular interstitial cells. Another 11 were selected for further screening by reverse dot blot hybridization, their size ranging from 200 to 500 bp, of which 6 were from the stages XIII - I segments of seminiferous tubules and the other 5 from the rat testicular interstitial cells. All of the 11 cDNA fractions were sub-cloned and screened by blue/white dots.</p><p><b>CONCLUSION</b>Specifically expressed differential cDNA fractions can be obtained and primarily identified from testicular interstitial cells and the seminiferous tubules, which, as the sequence tags of the testicular meiotic expression, deserve further investigation.</p>


Assuntos
Animais , Masculino , Ratos , Etiquetas de Sequências Expressas , Regulação da Expressão Gênica no Desenvolvimento , Células Intersticiais do Testículo , Biologia Celular , Meiose , Genética , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Túbulos Seminíferos , Biologia Celular , Espermatogênese , Genética
4.
Biomedical and Environmental Sciences ; (12): 153-164, 2004.
Artigo em Inglês | WPRIM | ID: wpr-329646

RESUMO

<p><b>OBJECTIVE</b>To investigate the anti-HIV effects of ampelopsin and its interaction with HIV-1 coreceptor CXCR4.</p><p><b>METHODS</b>Through anti-virus experiments in vitro, the inhibitory effect of ampelopsin on HIV-1 infection was verified. Chemotaxis assay was performed to show the ability to induce PBMCs migration by ampelopsin, RANTES and SDF-1alpha. Fluorescence labelling monoclonal antibody was utilized to observe the interaction of ampelopsin and CXCR4. Mice immunosuppressant model was also established to detail the role ampelopsin played in regulating cellular immunological functions.</p><p><b>RESULTS</b>Ampelopsin could protect sensitive cells against HIV-1 infection and dramatically reduce HIV-1 antigen P24 expression. HIV-1SF33 attaching to MT-4 cells was interfered by ampelopsin, and the EC50 was 0.175 mg/mL for cellular protection and 0.024 mg/mL for P24 inhibition. At co-cultivating phase, EC50 was 0.229 mg/mL and 0.197 mg/mL respectively. Furthermore, the EC50 was 0.179 mg/mL and 0.348 mg/mL in acute infection. Human PBMCs migration was induced after being challenged with ampelopsin or chemokines, and synergistic action was observed during co-treatment. Ampelopsin alone resulted in maximal chemotaxis at 1 mg/mL. HIV-1 co-receptor CXCR4 on the surface of PBMCs was decreased by internalization, which indicated the effect of ampelopsin on CXCR4. About 70% CXCR4 was reduced by ampelopsin at 1 mg/mL. Ampelopsin also augmented cellular immunological functions in immunosuppressive mice.</p><p><b>CONCLUSION</b>Ampelopsin displays a strong inhibitive role during HIV-1 absorption, incubation and acute infection. These results are coincident with its immune enhancement.</p>


Assuntos
Animais , Humanos , Camundongos , Ampelopsis , Química , Fármacos Anti-HIV , Farmacologia , Linhagem Celular , Quimiocina CCL5 , Farmacologia , Quimiocina CXCL12 , Quimiocinas CXC , Farmacologia , Quimiotaxia de Leucócito , Regulação para Baixo , Medicamentos de Ervas Chinesas , Flavonoides , Economia , Farmacologia , Infecções por HIV , Virologia , HIV-1 , Metabolismo , Virulência , Interleucina-2 , Leucócitos Mononucleares , Camundongos Endogâmicos BALB C , Modelos Animais , Raízes de Plantas , Química , Receptores CXCR4 , Baço , Alergia e Imunologia , Linfócitos T , Alergia e Imunologia
5.
China Journal of Chinese Materia Medica ; (24): 957-961, 2003.
Artigo em Chinês | WPRIM | ID: wpr-293679

RESUMO

<p><b>OBJECTIVE</b>To investigate the effects of ampelopsin on B16 melanoma's invasion and metastasis in vivo and in vitro.</p><p><b>METHOD</b>B16 mouse melanoma cells were injected into C57BL/6 mouse via tail lateral vein, which subsequently colonized into the animal lungs to form an experimental pulmonary metastasis of tumor cell. The ampelopsin was administered at 3 dosages by intraperitoneal injection daily for 18 days from the day before the cells injection. The B16 mouse melanoma cells were exposed to ampelopsin for 3 days. The effects of ampelopsin on invasion, migration and adhesion of B16 melanoma cells were evaluated with Transwell chambers or attachment with polycarbonate filters and reconstituted basement membrane (Matrigel).</p><p><b>RESULT</b>The number of metastases in the animals that were given ampelopsin 150, 200, and 250 mg x kg(-1) x d(-1) was significantly reduced as compared to the vehicle control (P<0.05), and the inhibition rates were 30.97%, 40.58%, and 61.16%, respectively. The ability of the ampelopsin treated B16 cells to invade the reconstituted basement membrane was decreased significantly (P<0.01), and the inhibition rates were 36.06%, 59.58%, and 79.09% at 20 micromol x L(-1), 40 micromol x L(-1) and 80 micromol x L(-1) concentration, respectively. Ampelopsin could also inhibit B16 cells migration through PVPF in the Transwell chambers, and the inhibition rates were 51.59%, 56.51%, and 66.75% at 20 micromol x L(-1), 40 micromol x L(-1) and 80 micromol x L(-1), respectively (P<0.01). The ability of adhesion of the B16 cells by ampelopsin treated cells on fibronectin, laminin, or Matrigel was decreased significantly.</p><p><b>CONCLUSION</b>Ampelopsin has anti-invasive and anti-metastatic effects on B16 melanoma.</p>


Assuntos
Animais , Masculino , Camundongos , Antineoplásicos Fitogênicos , Farmacologia , Adesão Celular , Linhagem Celular Tumoral , Movimento Celular , Flavonoides , Farmacologia , Lauraceae , Química , Neoplasias Pulmonares , Melanoma Experimental , Patologia , Camundongos Endogâmicos C57BL , Invasividade Neoplásica , Plantas Medicinais , Química , Ensaios Antitumorais Modelo de Xenoenxerto
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