A preliminary approach of covering corrugator with rectangle periosteous flap in rhytidectomy / 中华整形外科杂志

<p><b>OBJECTIVE</b>To evaluate the aesthetic outcome after application of rectangle periosteous flap to cover corrugator.</p><p><b>METHODS</b>On the basis of regular rhytidectomy, a inter-eyebrow periosteous flap is applied to cover the fascia flap of corrugator by means of turnover. This manipulation helps to separate the skin from the muscle and prevent the re-adherence between the skin and muscle.</p><p><b>RESULTS</b>The approach was applied on 15 cases. The follow-up ranged from 6 months to 1 year. The results were satisfactory.</p><p><b>CONCLUSIONS</b>The method is effective in elimination of inter-eyebrow crease.</p>

Cloning and expression of canine interferon-gamma gene in mouse SP2/0 cell line / 生物工程学报

Canine Interferon-gamma (CaIFN-gamma) cDNA was cloned from spleen T cells of dog by reverse transcription-polymerase chain reaction (RT-PCR). CaIFN-gamma cDNA were digested with Hind III and Not I, and inserted into pRc/CMV2 expression vector. The pRc/CMV2/CaIFN-gamma vector was sequenced, and predicted to produce a signal peptide of 23 amino acids and a mature protein of 143 amino acids with a molecular weight of 19 kD. Two potential N-glycosylation sites are located at positions 16 and 83 of the mature protein. Comparison of the CaIFN-gamma protein sequence with that of CaIFN-gamma reported from DDBJ/GenBank revealed a homology of 99%. To establish a long time expression system, pRc/CMV2/CaIFN-gamma vector was transfected into mouse SP2/0 cell line. The SP2/0 cells culture supernatants was harvested and the antiviral activity was measured following cytopathic-effect inhibition assay using Madin-Darby Canine Kidney (MDCK)-vesicular stomatitis virus(VSV) system. Initial transformants with G418 phenotype produced recombinant CaIFN-gamma titers ranging from 2,500 to 5,000 u/mL of culture medium.