HPLC-MS3 analysis of chemical constituents in Epimedium brevicornum / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents of effective part of Epimedium brevicornum.</p><p><b>METHOD</b>The sample was extracted with ethanol and purified by macroporous resin. The structures were identified by HPLC-MS3 experiments.</p><p><b>RESULT</b>Nine compounds were identified from the effective part of E. brevicornum.</p><p><b>CONCLUSION</b>The method is simple and rapid for the identification of the flavonoids from E. brevicornum.</p>

Determination of linderane in root tuber of Lindera aggregata by HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To provide scientific basis for quality control of Lindera aggregata.</p><p><b>METHOD</b>HPLC analytical method was established using a Lichrospher C18 column and acetonitrile-water (56:44) as the mobile phase, detected at 235 nm.</p><p><b>RESULT</b>The linear range of linderane is between 0.0642 - 0.5774 microg, the average recovery was 98.4%, RSD1.7% (n = 9).</p><p><b>CONCLUSION</b>Contents of linderane in commercially available and collected samples were from 0.028% to 0.123% and from 0.056% to 0.222% respectively.</p>

Detection of hepatotoxic pyrrolizidine alkaloids in Ligularia Cass. with LC/MSn / 药学学报

<p><b>AIM</b>To detect the hepatotoxic pyrrolizidine alkaloids (HPA) in the genus Ligularia Cass..</p><p><b>METHODS</b>The alkaloid extracts of Ligularia plant materials were detected and analyzed by the method of combination of TLC, and LC/MSn.</p><p><b>RESULTS</b>Among 22 species of Ligularia Cass., HPA were detected in 18 species with LC/MSn, and no HPA was detected in the remaining 4 species.</p><p><b>CONCLUSION</b>HPA was first detected with LC/MSn in L. tongelensis and other 15 species of Ligularia Cass.; HPA from these plants should be isolated, separated and identified and it is necessary to study the activities and toxicities of the HPA. The types and kinds of HPA from different species and sources are different, they should be detected separately.</p>