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Chinese Journal of Pathology ; (12): 232-236, 2006.
Artigo em Chinês | WPRIM | ID: wpr-277436

RESUMO

<p><b>OBJECTIVE</b>To study further the most important and frequent genetic alterations of p53 and epidermal growth factor receptor (EGFR) in astrocytic gliomas.</p><p><b>METHODS</b>(1) EGFR expression was examined in samples collected from 37 astrocytic gliomas and 6 normal brain tissue using reverse transcriptase polymerase chain reaction and immunohistochemical staining. (2) p53 gene mutation and accumulation were detected simultaneously in the same specimens using PCR-SSCP, DNA sequencing and immunohistochemical staining.</p><p><b>RESULTS</b>The frequency of p53 mutation in diffuse astrocytomas, anaplastic astrocytomas, primary glioblastomas and secondary glioblastomas was 1/10, 4/19 (21.1%), 4/6 and 2/2, respectively and the frequency of EGFR overexpression was 5/10, 10/19 (52.6%), 5/6 and 2/2, respectively. Both p53 accumulation and EGFR overexpression increased accompanied by a successive increase of degree of the glioma malignancy.</p><p><b>CONCLUSIONS</b>EGFR overexpression is not infrequently seen, however, p53 mutation is rarely seen in the low grade gliomas. Both p53 gene mutation and EGFR overexpression are often associated with primary and secondary glioblastoma. Consequently, EGFR overexpression and p53 gene mutation are not mutually exclusive in astrocytic gliomagenesis but synergistically to promote the glioma progression.</p>


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Astrocitoma , Genética , Metabolismo , Patologia , Sequência de Bases , Neoplasias Encefálicas , Genética , Metabolismo , Patologia , Análise Mutacional de DNA , Regulação Neoplásica da Expressão Gênica , Glioblastoma , Genética , Metabolismo , Patologia , Imuno-Histoquímica , Dados de Sequência Molecular , Mutação , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita Simples , RNA Mensageiro , Genética , Receptores ErbB , Genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Proteína Supressora de Tumor p53 , Genética
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