Determination of fraxinellone in root bark of Dictamnus dasycarpus by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To develop a RP-HPLC method for determination of fraxinellone in Dictamnus dasycarpus.</p><p><b>METHOD</b>RP-HPLC conditions were as follows: AT-LICHROM C18(4.6 mm x 250 mm) column, MeOH-H2O (70:30) as a mobile phase, detection wavelength 240 nm, column temperature 25 degrees C, Flow velocity 0.5 mL x min(-1).</p><p><b>RESULT</b>Linear relationship is very good (r = 0.9999) in 0.018-0.18 mg x mL(-1). The average recovery is 98.9%, RSD is 1.2% (n = 4).</p><p><b>CONCLUSION</b>The method is simple and repeatable and can be used for quantitative analysis of fraxinellone in root bark of D. dasycarpus.</p>