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1.
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-954723

RESUMO

Background Despite the high importance of Helicobacter pylori, the origin and transmission of this bacterium has not been clearly determined. According to controversial theories and results of previous studies, animal source foods - especially milk - play an important role in the transmission of H. pylori to humans. The aim of the present study was to determine the distribution of vacA, cagA,iceA and oipA virulence factors inH. pylori strains isolated from milk and dairy products and study their antimicrobial resistance properties.Methods A total of 520 raw milk and 400 traditional dairy product samples were cultured and tested. Those that were H. pylori-positive were analyzed for the presence of vacA,cagA, iceA and oipAvirulence factors. Antimicrobial susceptibility testing was performed by the disk diffusion method.Results One hundred and three out of 520 milk samples (19.8%) and 77 out of 400 dairy products samples (19.2%) were contaminated with H. pylori. The most frequently contaminated samples were ovine milk (35%) and traditional cheese (30%). Total prevalence ofvacA, cagA, iceA andoipA factors were 75%, 76.6%, 41.6% and 25%, respectively. H. pylori strains of milk and dairy products harbored high levels of resistance to ampicillin (84.4%), tetracycline (76.6%), erythromycin (70.5%) and metronidazole (70%).Conclusions High presence of antibiotic-resistant strains of H. pylorisuggest that milk and dairy samples may be the sources of bacteria that can cause severe infection. Our findings should raise awareness about antibiotic resistance in H. pylori strains in Iran.(AU)


Assuntos
Animais , Resistência Microbiana a Medicamentos , Helicobacter pylori , Fatores de Virulência , Leite , Antibacterianos
2.
J. venom. anim. toxins incl. trop. dis ; 20: 1-7, 04/02/2014. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1484598

RESUMO

Background Despite the high importance of Helicobacter pylori, the origin and transmission of this bacterium has not been clearly determined. According to controversial theories and results of previous studies, animal source foods especially milk play an important role in the transmission of H. pylori to humans. The aim of the present study was to determine the distribution of vacA, cagA,iceA and oipA virulence factors inH. pylori strains isolated from milk and dairy products and study their antimicrobial resistance properties. Methods A total of 520 raw milk and 400 traditional dairy product samples were cultured and tested. Those that were H. pylori-positive were analyzed for the presence of vacA,cagA, iceA and oip Avirulence factors. Antimicrobial susceptibility testing was performed by the disk diffusion method.Results One hundred and three out of 520 milk samples (19.8%) and 77 out of 400 dairy products samples (19.2%) were contaminated with H. pylori. The most frequently contaminated samples were ovine milk (35%) and traditional cheese (30%). Total prevalence ofvacA, cagA, iceA andoipA factors were 75%, 76.6%, 41.6% and 25%, respectively. H. pylori strains of milk and dairy products harbored high levels of resistance to ampicillin (84.4%), tetracycline (76.6%), erythromycin (70.5%) and metronidazole (70%). Conclusions High presence of antibiotic-resistant strains of H. pylorisuggest that milk and dairy samples may be the sources of bacteria that can cause severe infection. Our findings should raise awareness about antibiotic resistance in H. pylori strains in Iran.


Assuntos
Animais , Anti-Infecciosos/análise , Farmacorresistência Bacteriana , Fatores de Virulência , Helicobacter pylori , Laticínios/efeitos adversos , Leite/efeitos adversos , Irã (Geográfico)
3.
Biol. Res ; 47: 1-13, 2014. tab
Artigo em Inglês | LILACS | ID: biblio-950724

RESUMO

BACKGROUND: Calf diarrhea is a major economic concern in bovine industry all around the world. This study was carried out in order to investigate distribution of virulence genes, pathotypes, serogroups and antibiotic resistance properties of Escherichia coli isolated from diarrheic calves. RESULTS: Totally, 76.45% of 824 diarrheic fecal samples collected from Isfahan, Chaharmahal, Fars and Khuzestan provinces, Iran were positive for E. coli and all of them were also positive for cnf2, hlyA, cdtIII, f17c, lt, st, stx1, eae, ehly, stx2 and cnf1 virulence genes. Chaharmahal had the highest prevalence of STEC (84.61%), while Isfahan had the lowest (71.95%). E. coli serogroups had the highest frequency in 1-7 days old calves and winter season. Distribution of ETEC, EHEC, AEEC and NTEC pathotypes among E. coli isolates were 28.41%, 5.07%, 29.52% and 3.49%, respectively. Statistical analyses were significant for presence of bacteria between various seasons and ages. All isolates had the high resistance to penicillin (100%), streptomycin (98.25%) and tetracycline (98.09%) antibiotics. The most commonly detected resistance genes were aadA1, sul1, aac[3]-IV, CITM, and dfrA1. The most prevalent serogroup among STEC was O26. CONCLUSIONS: Our findings should raise awareness about antibiotic resistance in diarrheic calves in Iran. Clinicians should exercise caution when prescribing antibiotics.


Assuntos
Animais , Bovinos , Doenças dos Bovinos/microbiologia , Proteínas de Escherichia coli/genética , Farmacorresistência Bacteriana/genética , Diarreia/veterinária , Escherichia coli/patogenicidade , Estações do Ano , Virulência/genética , Resistência ao Cloranfenicol/genética , Sorotipagem , Reação em Cadeia da Polimerase , Prevalência , Fatores Etários , Primers do DNA , Diarreia/microbiologia , Diarreia/epidemiologia , Escherichia coli/efeitos dos fármacos , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Sorogrupo , Irã (Geográfico)/epidemiologia
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