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Objective To investigate the hypoglycemic effects of Laminaria japonica (L. japonica) on diabetic model induced by alloxan in rats. Methods Sixty healthy female rats were used to establish diabetic models by injecting alloxan peritoneally, and L.japonica was applied as raw materials for potential marine drugs.The levels of fasting blood glucose (FBG) were detected by automatic blood glucose device. Enzyme linkedimmunoabsorbant assay was applied to determine the insulin level in serum. The shape and structure of isletcells were observed with histopathological staining, and the expression of superoxide dismutase (SOD) and inducible nitric oxide synthase (iNOS) in islet cells were detected by immunohistochemical technique. Results After the treatment, the levels of FBG of L.japonica treated group B [(9.37±1.70) mmol/LandC (9.18±1.65 ) mmol/L, F= 32.81, q=6.35～11.72, P<0.05 ] reduced, while the serum levels of insulin in treated group A, Band C (0.0378±0.0026, 0.0378±0.0027, 0.0367±0.0035) increased(F= 11.40, q=4.28～8.47, P<0.05) significantly than those of diabetic model group (0.0456 ±0.0057) . The shape and structure of islet cells improved with the up-expressing SOD(t=4.73～4.76, P<0.05)and down-expressing iNOS (t=4.81～5.30, P<0.05) in L.japonica treated group B and C than those in diabetic model group. Conclusion L.japonica might decrease the serum level of FBG through promoting the islet cell recovery by an anti-oxide effect.
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Objective To study the best protecting dosage of phycocyanin on PC12 cells after anoxia/re-oxygen in vitro.Methods The anoxia/re-oxygen cell models were established with cultured PC12 cells and treated by phycocyanin.The absorbency of PC12 cells was determined by MTT method to observe the activity and number of PC12 cells after anoxia/re-oxygen.The fluorescent intensity in PC12 cells labeled by Rhodamine123 was measured with laser scanning confocal microscope to observe the mitochondrial membrane potential after anoxia/re-oxygen.Results After anoxia/re-oxygen,the absorbency and fluorescent intensity in PC12 cells were significantly lower than those in control group.After treatment with phycocyanin,the absorbency of PC12 cell in test groups was higher than that in model group,especially in 5?g?mL-1 subgroup,P
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Objective To investigate the protective effect of phycocyanin on focal cerebral ischemia reperfusion in rats.Methods The model of the middle cerebral artery occlusion and reperfusion(MCAO/R) was established using the intraluminal filament occlusion with 60 healthy adult male Wistar rats,and treated by phycocyanin.The expressions of NF-?B,IL-6 and neuronal apoptosis were detected to evaluate the effects of phycocyanin on above indexes.Results The expression level of NF-?B increased successfully from reperfusion 6h,peaked at reperfusion 1d,and then decreased in cortex and striatum in ischemic group.The expression site and change principle of IL-6 were similar to those of NF-?B except of expression peak at reperfusion 2d.The neuronal apoptosis increased successfully from reperfusion 6h,reached peak at reperfusion 1d and 2d in cortex and striatum respectively,and the changed character was coincident with NF-?B and IL-6 expressions.The expressions of NF-?B and IL-6 reduced and the number of neuronal apoptosis decreased significantly after applying phycocyanin compared to the control group in the same time.Conclusion Phycocyanin could inhibit neuronal apoptosis and down-regulate the expressions of NF-?B and IL-6 after cerebral ischemia-reperfusion.