RESUMO
Object To determine the amount of acacetin-7-O-β-D-glucoside and apigenin-7-O-β-D-glucoside in Chrysanthemum morifolium Ramat. from different localities. Methods By the use of HPLC. Results The contents of acacetin-7-O-β-D-glucoside in Boju1, Chuju2, Gongju3 and Hangju4 were 0.082 6%～0.124 9%, 0.015 7% and 0.033 5% respectively; the contents of apigenin-7-O-β-D-glucoside in Chuju, Boju, Gongju and Hangju were 0.012 6%, 0.027 4%, 0.117 7% and 0.587 7% respectively. Conclusion The contents of the two flavone glycoside in C. morifolium from different localities were markedly different.
RESUMO
Objective: To determine luteolin 7 O ? D glucoside in Chrysanthemum morirolium Ramat. Methods: The content of luteolin 7 O ? D glucoside was determined by HPLC on Symmetry Shield TM RP S column with MeoH H 2O(49∶51) as a mobile phase and detection wavelength at 348nm. Results: The liuear relationship of this method was well and the average recovery of the added sample is 99.74%. Conclusion: The analytical time is short, separating degree is good and results are accurate when luteolin 7 O ? D glucoside is determined by this method.
RESUMO
In order to pursue enkephalin studies by means of radioimmunoassay and immunohistochemical methode, We prepared anti-leu-enkephalin serum (ALS) and anti-met-enkephalin (AMS).The procedure was as follows: Rabbits were immunized with respective enkephalin immunogen of home and foreign make. The specific antiserum was thus obtained. The antiserum possessed satisfactory sensitivity, specificity and repla-ceability for RIA. In the competitive inhibition curves the minimal detactable limit of ALS was 25 pg and that of AMS 60 pg.In the cross test reactions with seven kinds of peptide hormones and morphine, no significant interference was found. The cross reaction rate of LEK vs AMD was 0.44%, and that of AMS vs LEK 2.8%. According to Cerceo's method of power-point system, both ALS and AMS were reliable antiserums in our experiments.