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2.
Indian J Exp Biol ; 1998 Jun; 36(6): 553-8
Artigo em Inglês | IMSEAR | ID: sea-62300

RESUMO

Enhancing factor (EF) protein was initially purified as a modulator of epidermal growth factor from small intestines of mouse. The cDNA sequence, obtained by RT-PCR, revealed that EF belonged to the non-pancreatic, phospholipase A2 (PLA2) family. This was the first report of the mouse PLA2. In the present paper we report the complete cDNA sequence of EF gene, in which the 5' sequence has been obtained by RAcE-PCR. The predicted amino acid sequence was computer analysed and the putative sites for enzyme action, calcium binding and heparin binding have been identified. The complete protein sequence of EF along with 16 aligned sequences were used to infer a phylogenetic tree. From this data the mouse EF was grouped with other membrane associated PLA2 with a bootstrap value of 98% indicating that it belonged to this class.


Assuntos
Sequência de Aminoácidos , Animais , Sequência de Bases , DNA Complementar , Fosfolipases A2 do Grupo II , Humanos , Camundongos , Dados de Sequência Molecular , Fosfolipases A/genética , Fosfolipases A2 , Filogenia
4.
J Postgrad Med ; 1991 Apr; 37(2): 93-6
Artigo em Inglês | IMSEAR | ID: sea-116706

RESUMO

A number of plasmids carrying the Lac+ character have been reported. Lac+ character of salmonella S:41:z10:- studied for transfer of Lac+ character to standard Escherichia coli K12 Lac-F- Nalr and Escherichia coli K12 F- Lac- Rifr, failed to transfer in in vitro experiments. Similarly, identification and characterisation of plasmid DNA by agarose gel electrophoresis technique did not show specific plasmid DNA as compared to standard molecular weight plasmids. Plasmid DNA appeared to have been embedded with chromosomal DNA molecule.


Assuntos
Animais , Anuros , Conjugação Genética , Escherichia coli/genética , Fatores de Lactose/genética , Plasmídeos/genética , Salmonella/genética
5.
Indian Pediatr ; 1988 Sep; 25(9): 893-7
Artigo em Inglês | IMSEAR | ID: sea-8873
11.
J Postgrad Med ; 1982 ; 28(4): 181-3
Artigo em Inglês | IMSEAR | ID: sea-115874
12.
J Postgrad Med ; 1978 Oct; 24(4): 226-30
Artigo em Inglês | IMSEAR | ID: sea-116542
14.
16.
J Postgrad Med ; 1971 Jan; 17(1): 31-4
Artigo em Inglês | IMSEAR | ID: sea-115198
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