Research advances in active components of traditional Chinese medicine in prevention and treatment of nonalcoholic fatty liver disease based on oxidative stress / 临床肝胆病杂志

Nonalcoholic fatty liver disease (NAFLD) is a multifactorial pathological disease. Although the molecular mechanism of the onset of NAFLD has not been fully elucidated, oxidative stress is believed to play a key role in this process and can affect a variety of physiological functions. In recent years, the use of active components of traditional Chinese medicine in the prevention and treatment of NAFLD has become a research hotspot. This article summarizes the role of active components of traditional Chinese medicine in the treatment of NAFLD from the perspective of anti-oxidative stress effect, so as to provide a scientific basis for the prevention and treatment of NAFLD.

Efficacy of flupentixol and melitracen in optimizing conventional treatment for postherpetic neural-gia / 中华麻醉学杂志

Objective To evaluate the efficacy of flupentixol and melitracen in optimizing conven-tional treatment for postherpetic neuralgia. Methods Seventy patients of both sexes with thoracolumbar postherpetic neuralgia, were divided into 2 groups ( n＝35 each) according to the registration order: pa-tients with odd number were included in control group ( group C) and patients with even number were in-cluded in flupentixol-melitracen group (group D). Patients in group C received conventional treatment: an-ti-epileptic drugs, analgesia with opioids, neurotrophy, paravertebral nerve block and physical therapy. Flupentixol-melitracen 10. 5 mg was taken orally based on the conventional treatment in group D. The time for treatment was recorded. The severity of pain was assessed by using the numeric rating scale, and anxiety and depression were evaluated using the Hospital Anxiety and Depression Scale before treatment and on 3rd and 7th days after treatment. The development of flupenthixol and melitracen-related adverse reactions was recorded during treatment in group D. Results Compared with group C, the numeric rating scale and Hos-pital Anxiety and Depression Scale scores were significantly decreased on 3rd and 7th days after treatment, and the time for treatment was shortened in group D (P＜0. 05). No flupenthixol-and melitracen-related ad-verse reactions were found in group D. Conclusion Flupentixol-melitracen can optimize the conventional therapeutic effect for postherpetic neuralgia.

Effect of sodium butyrate combined with TRAIL on biological behaviors of lung cancer stem cells / 中国组织工程研究

BACKGROUND:Sodium butyrate, a histone deacetylase inhibitor, can inhibit cell proliferation, and induce apoptosis and differentiation of various cancer cells. However, the role of sodium butyrate combined with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on lung cancer stem cells remains unclear. OBJECTIVE:To explore the effect of sodium butyrate combined with TRAIL on biological behaviors of lung cancer stem cells. METHODS:Magnetic bead separation was used to separate lung cancer stem cells (CD133+) from human lung adenocarcinoma A549 cells. After the lung cancer stem cells were treated with simple DMEM/F12, DMEM/F12 containing sodium butyrate (5 mmol/L), TRAIL (50 μg/L) or sodium butyrate combined with TRAIL, the cell proliferation within 96 hours of culture was determined by MTT assay; the apoptosis within 24 hours of culture was measured by flow cytometry; the cell migration within 48 hours of culture was detected by cell scratch test; the expression levels of pluripotent transcription factors (Oct4, Sox2 and Nanog) within 48 hours of culture were detected using western blot analysis. RESULTS AND CONCLUSION:The CD133+ lung cancer stem cells were successfully enriched from human lung adenocarcinoma A549 cells. MTT assay showed that sodium butyrate and TRAIL significantly inhibited the proliferation of lung cancer stem cells (P< 0.05), and the combination effect was even stronger (P < 0.05). Results from flow cytometry analysis and scratch test showed that sodium butyrate or TRAIL induced apoptosis and inhibited cell migration of lung cancer stem cells (P < 0.05), and the combination of sodium butyrate and TRAIL showed a stronger effect (P < 0.05). In addition, the expression levels of Oct4, Sox2 and Nanog were significantly down-regulated by sodium butyrate (P < 0.05), TRAIL or sodium butyrate combined with TRAIL, and the combination effect was stronger (P < 0.05). In conclusion, sodium butyrate and TRAIL have synergistic effects on lung cancer stem cells, indicating a new way for treatment of lung cancer.

Progress in Research on Bowel Preparation for Colon Capsule Endoscopy / 胃肠病学

Colon capsule endoscopy( CCE)is a new technique for the diagnosis of colonic lesions,it has many advantages such as high safety,good tolerance,etc. . Bowel preparation is an important procedure in CCE. The quality of bowel preparation is closely related with the accuracy of CCE. This article reviewed the progress in research on bowel preparation for CCE.

Protective Effect of Momordica Saponins Compounds on the Kidney of Type 2 Diabetic Mice / 医药导报

Objective To study the protective effects of momordica saponins compounds on the kidney of type 2 diabetic mice. Methods The mouse model of type 2 diabetes was induced by feeding high￣fat diet for a week,followed by intraperitoneal injection of streptozotocin(STZ)at 150 mg.kg-1 .The mice were randomly divided into groups of normal control group(A), model control group ( B), rosiglitazone group ( C, 0. 4 mg . kg-1 ), the compounds at low￣dosage group ( D, 350 mg.kg-1 ) and high￣dosage group(E,700 mg.kg-1 ).The levels of fasting blood glucose,UCr,SCr,TGF￣β1 ,mALB,and HbAlc were determined respectively after being treated for 12 weeks. Results Compared with normal control group,the level of fasting blood glucose,SCr,TGF￣β1 ,mALB,and HbAlc in all other groups increased significantly (P<0.01);Compared with model control group,the level of fasting blood glucose,SCr,TGF￣β1 ,mALB and HbAlc in all treatment groups decreased significantly (P<0.01) ,the level of UCr increased markedly (P<0.01). Conclusion The Momordica saponins compounds can inhibit renal injury and exert kidney protective effect on type 2 diabetic mice.

Inhibitory effect of mouse angiostatin cDNA gene transduction on human liver cancer cell / 中华消化杂志

Objective To study the effect of the mouse angiostatin cDNA gene transfecting into human liver cancer cell line HCC7721 on cell growth, cell cycle phase distribution, cell morphology in vitro and tumorigenesis in vivo , and the mechanisms. Methods The gene fragment of mouse angiostatin cDNA was directly cloned into an eukaryotic expression plasmid pcDNA3.1(+) of the promoter CMV between the multicloning sites HindⅢ and XbaⅠ and confirmed the correct recombinant plasmid pcDNA3.1(+) angio through enzymatic digestion and gene sequencing. Then it was transfected into human liver cancer cell line HCC7721 with liposome, pcDNA3.1(+) as vecter control and liposome as mock control. After 30 day selection by neomycin G418, angiostatin expression at the levels of mRNA and protein in vitro were tested by RNA dot blot and FACS respectively. Cell morphology under was observed light microscope, made growth curves were made, and cell cycle distribution checked in FACS. Animal model was set up to study tumorigenesis in vivo of those cells transplanted subcutaneously in the right hind legs of nude mice BALBc. Microvessel density (MVD) was analyzed and angiostatin expression in the tumor tissues by in situ immunohistochemistry. Results The recombinant eukaryotic expression plasmid pcDNA3.1(+) angio was constructed Auccessfully. After selection of G418 for about 4 weeks there were macroscopic cell clones in the experimental group and vector control group, but no survival cells in the mock control. In vitro angiostatin were expressed in the experimental group, but not in the vector control. There weren't significant changes in cell morphology, cell growth curves and cell cycle distribution between the experimental and the control group. However, the nude mice experiment showed that tumorigenic capability of the experimental cells had been reduced greatly. Immunochemistry study showed that there were much less microvessels and conversely stronger angiostatin positive staining in the tumor tissues than in the control group. Conclusions Mouse angiostatin has no direct inhibition on human liver cancer cell line HCC7721 in vitro , but it inhibits effectively the tumorigenesis of HCC7721 in vivo , which is probably due to its inhibition on tumor angiogenesis in a paracrine path way.

Smoking Behavior and Self-Esteem of Students of Private Schools / 中国心理卫生杂志

Objective:To study the relationship between smoking behavior and self-esteem of adolescents.Methods:1307 students of the seventh and eighth grades of 8 private schools completed questionnaires about smoking behavior and self-esteem. Results: More cigarettes smoking, less score of self-esteem. Those had quit smoking had higher scores in self-esteem than those can not quit. Students who chose“will smoke in the future”had lower self-esteem than the students chose“won't smoke in the future”.Conclusion:Smoking behavior is a marker of low self-esteem.

Protective effect of large doses of vitamin C on acute lung injury / 西安交通大学学报(医学版)

Objective To investigate the protective and therapeutic effect of large doses of vitamin C on the acute lung injury in the model of rabbits induced by oleic acid. Methods A total of 18 rabbits were divided into four group randomly: control group(CG); oleic acid group(OG); preventive group(PG) and treatment group(TG). Oxygen pressure in arterial blood (PaO_(2)), superoxide dismutase (SOD) and malondialdehyde(MDA) in blood were measured in 0, 30, 90 and 150min, respectively. All the animals were killed in 150min later. Total protein in broncho-alveolar lavage fluid (BALF) and wet dry weight ratio of the right lung were measured, and the left lung sample was treated by formaldehyde for pathological test. Results PaO_(2), SOD in PG group and TG group were significantly higher than that in OG group(P