Identification of constituents in vitro and blood-absorbed ingredients of protective effect on acute liver injury from Yin Chen Hao decoction based on UPLC-QTOF/MS / 药学学报

To identify the active constituents in vitro and blood-absorbed ingredients in vivo from Yin Chen Hao decoction provides scientific evidence for probing its prevention and treatment mechanism on acute liver injury. An ultrahigh performance liquid chromatography quadrupole-time of flight-mass spectrometry (UPLC-QTOF/MS) method was applied for analysis of Yin Chen Hao decoction and the serum samples of mice with con-A induced acute liver injury after preventive oral administration for 14 days (the use of all laboratory animals in this study was approved by the Ethics Committee of the Naval Medical University, 19YF1459400). A total of 90 chemical constituents were identified from Yin Chen Hao decoction, mainly were flavonoids, terpenoids, tannins, quinones. 5 prototype compounds were identified in the serum, including chrysophanol, deoxyrhapontin-8-O-gallate, mussaenosidic acid, herniarin, emodin. The established UPLC-QTOF/MS method could efficiently and sensitively identify the constituents in vitro and blood-absorbed ingredients of Yin Chen Hao decoction, primarily clarify the material basis of its hepatoprotective effect, and provided a scientific basis for the quality marker selection and the pharmacodynamic material basis research on the decoction.

Headspace GC-MS for fast identification of Rhizoma curcuma of different origins / 第二军医大学学报

Objective: To distinguish Rhizoma curcuma of different origins using headspace-gas chromatography/mass spectrometry (HS-GC/MS) combined with principal components analysis (PCA) and hierarchical cluster analysis (HCA), so as to help the quality control of Rhzzoma curcuma. Methods: HP-5 capillary column (30 m×0. 32 mm, 0. 25 μm) was used under the following condition; inlet temperature; 250°C, initial column temperature; 50°C maintained for 3 min, then increasing to 150°C at 20°C/min and to 200°C at 2°C/min, maintained for 10 min, with the split ratio being 10 : 1. The carrier gas was helium, with flow rate being 1. 0 mL/min, had space vials regional temperature being 90°C, vials heating equilibration time being 30 min, and injection volume being 1. 5 mL. The effect of extract separation conditions, temperature of the vial and equilibrium time on the extraction volatile components of RMzoma curcuma were observed. Results: PCA could distinguish 18 common peaks of 15 batches of Rhizoma curcuma from Sichuan, Guangxi and Yunnan, and it was confirmed that (3-elemene, camphene, 13-pinene, p-menth-l-en-8-ol, eucalyptol, and cycloisolongifolene, 8, 9-dehydro-9-formyl were the main components to cause differences in RMzoma curcuma of different origins. Conclusion: We have established a method combining HS-GC/MS with PCA and HCA to distinguish RMzoma curcuma of different origins, and we have also identified the major characteristic components of Rhizoma curcuma of different origins.