Differentiation of human amniotic mesenchymal stem cells into insulin-secreting cells induced by regenerating pancreatic extract / 生物工程学报

In this study, the natural biological inducer, rat regenerating pancreatic extract (RPE), was used to induce human amniotic mesenchymal stem cells (hAMSCs) into insulin-secreting cells. We excised 60% of rat pancreas in order to stimulate pancreatic regeneration. RPE was extracted and used to induce hAMSCs at a final concentration of 20 microg/mL. The experiment methods used were as follows: morphological-identification, dithizone staining, immumofluorescence analysis, reverse transcription-PCR (RT-PCR) and insulin secretion stimulated by high glucose. The results show that the cell morphology of passge3 hAMSCs changed significantly after the induction of RPE, resulting in cluster shape after induction for 15 days. Dithizone staining showed that there were scarlet cell masses in RPE-treated culture. Immumofluorescence analysis indicated that induced cells were insulin-positive expression. RT-PCR showed the positive expression of human islet-related genes Pdx1 and insulin in the induced cells. The result of insulin secretion stimulated by high glucose indicated that insulin increasingly secreted and then kept stable with prolongation of high glucose stimulation. In conclusion, hAMSCs had the potential to differentiate into insulin-secreting cells induced by RPE in vitro.

Study on anticoagulant and thrombolytic effects of a novel marine fibrinolytic enzyme in vitro / 中国海洋药物

Objective To estimate the anticoagulant and thrombolytic effects of a novel fibrinolytic enzyme with a low molecular weight for further carrying on animal test and clinic research.Methods Using pH7.4,0.9 % NaCl as negative control and lumbrukinase as positive control,the anticoagulant and thrombolytic effects of the novel marine fibrinolytic enzyme were investigated in vitro with fresh blood from wistar rat and rabbit,respectively.Results The results showed that there were apparent anticoagulant effect as well as thrombolytic effects in the self-made marine fibrinolytic enzyme,and the status of the released blood cells from thrombus using this enzyme was also better than that of using lumbrukinase.Conclusion The novel self-made fibrinolytic enzyme is a potent thrombolytic agent.