Secretion of laccase and manganese peroxidase by Pleurotus strains cultivate in solid-state using Pinus spp. sawdust

Pleurotus species secrete phenol oxidase enzymes: laccase (Lcc) and manganese peroxidase (MnP). New genotypes of these species show potential to be used in processes aiming at the degradation of phenolic compounds, polycyclic aromatic hydrocarbons and dyes. Hence, a screening of some strains of Pleurotus towards Lcc and MnP production was performed in this work. Ten strains were grown through solid-state fermentation on a medium based on Pinus spp. sawdust, wheat bran and calcium carbonate. High Lcc and MnP activities were found with these strains. Highest Lcc activity, 741 ± 245 U gdm-1 of solid state-cultivation medium, was detected on strain IB11 after 32 days, while the highest MnP activity occurred with strains IB05, IB09, and IB11 (5,333 ± 357; 4,701 ± 652; 5,999 ± 1,078 U gdm-1, respectively). The results obtained here highlight the importance of further experiments with lignocellulolytic enzymes present in different strains of Pleurotus species. Such results also indicate the possibility of selecting more valuable strains for future biotechnological applications, in soil bioremediation and biological biomass pre-treatment in biofuels production, for instance, as well as obtaining value-added products from mushrooms, like phenol oxidase enzymes.

Isolation of cellulase-producing mutants from a Penicillium sp strain denominated 3MUV24

Três mutantes celuloliticos foram obtidos de uma linhagem de Penicillium sp denominada 3MUV24, um espontaneo e dois após mutagênese com luz ultravioleta (254 nm). A seleçäo dos mutantes foi desenvolvida em placas contendo meio sólido com celulose. Os mutantes foram detecytados como aqueles que produziram áreas maiores de clareamento nas placas de celulose. A produçäo de FPA (atividade de hidrolise em papel de filtro) e beta-glicosidase por uma das linhagens mutantes, desenvolvida a 28 grade C em frascos agitados de 500 ml com 100 ml de meio, foi aproximadamente 2 vezes e 6 vezes mais alta respectivamente, do q ue a linhagem parenteral