1.
J Biosci
; 2001 Dec; 26(5): 635-40
Artigo
em Inglês
| IMSEAR
| ID: sea-111330
RESUMO
The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.
Assuntos
Western Blotting , Clonagem Molecular , Concanavalina A/química , Fabaceae/química , Lectinas de Plantas , Precursores de Proteínas/química , Proteínas Recombinantes/química , Solubilidade
2.
Artigo
em Inglês
| IMSEAR
| ID: sea-111176